Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens

Neupane, Mary; Sudeep, K C; Thakur, Subash; Panta, Om Prakash; Joshi, Dev Raj; Khanal, Santosh

doi:10.3126/tujm.v6i0.26583

Cited by 3 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, all three types of -lactamases -AmpC, KPC, and ESBL -were produced by 3.8% of isolates. According to the results of other study, 1.78% of the isolates produced all three types of -lactamases (23).…”

Section: Discussionmentioning

confidence: 77%

Associations Between β-Lactamase Types of Acinetobacter Baumannii and Antimicrobial Resistance

Černiauskienė¹,

Dambrauskienė²,

Vitkauskienė³

2023

Preprint

View full text Add to dashboard Cite

Background and objective: Acinetobacter baumannii (A. baumannii) is an important nosocomial pathogen that possesses not only intrinsic resistance to many classes of antibiotics, but also is capable to rapidly develop antimicrobial resistance during treatment. The aim of our study was to determine the production of different types of β-lactamases (AmpC, ESBL, KPC) in A. baumannii strains, to evaluate its association with antimicrobial resistance, and to identify the changes in these characteristics after 5 years. Materials and methods: A total of 233 A. baumannii strains were isolated from different clinical specimens of patients treated at the Hospital of Lithuanian University of Health Sciences in 2016–2017 and 2021–2022. All clinical cultures positive for A. baumannii were analyzed. The type of β-lactamase was detected by phenotypic methods using ESBL plus AmpC screen disk tests and the combination meropenem disk test. Results: In 2016–2017 and 2021–2022, all A. baumannii strains were resistant to ciprofloxacin; more than 80% were resistant to carbapenems, piperacillin/tazobactam, gentamicin, and tobramycin. Comparison of two different period showed that the production of two different types of β-lactamases increased more than 5-fold (from 17.7% to 94.2%, p < 0.001), and the production of all three types of β-lactamases, more than 4-fold (from 1.3% to 5.8%, p < 0.001). A. baumannii strains producing two or three types of β-lactamases were more often resistant to tigecycline, tetracycline, and doxycycline than strains producing one type of β-lactamases (p < 0.001). Conclusions: The frequency of isolation of A. baumannii strains producing two or three β-lactamases types and the resistance rates to ampicillin/sulbactam, tigecycline, tetracycline, and doxycycline increased in 2020–2021 as compared with 2016–2017. The production of two or three types of β-lactamases by A. baumannii strains was associated with higher resistance rates to tetracyclines.

show abstract

Section: Discussionmentioning

confidence: 77%

Associations Between β-Lactamase Types of Acinetobacter Baumannii and Antimicrobial Resistance

Černiauskienė¹,

Dambrauskienė²,

Vitkauskienė³

2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In our study, all three types of β -lactamases—AmpC, KPC, and ESBL—were produced by 3.8% of isolates. According to the results of other research, carried out in in a tertiary care hospital of South India, 1.78% of the isolates produced all three types of β -lactamases [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Associations between β-Lactamase Types of Acinetobacter baumannii and Antimicrobial Resistance

Černiauskienė,

Dambrauskienė,

Vitkauskienė

2023

Medicina

View full text Add to dashboard Cite

Background and objective: Acinetobacter baumannii (A. baumannii) is an important nosocomial pathogen that not only possesses intrinsic resistance to many classes of antibiotics, but is also capable of rapidly developing antimicrobial resistance during treatment. The aim of this study was to determine the characteristics of resistance of A. baumannii strains to β-lactams and other tested antibiotics, to evaluate the associations between the phenotypes of resistance to β-lactams and other tested antibiotics, and to evaluate the changes in antibiotic resistance of A. baumannii strains over 5 years by comparing the periods of 2016–2017 and 2020–2021. Materials and methods: A total of 233 A. baumannii strains were isolated from different clinical specimens of patients treated at the Hospital of Lithuanian University of Health Sciences in 2016–2017 (n = 130) and 2021–2022 (n = 103). All clinical cultures positive for A. baumannii were analyzed. The type of β-lactamase was detected by phenotypic methods using ESBL plus AmpC screen disk tests and the combination meropenem disk test. Results: In both periods, all A. baumannii strains were resistant to ciprofloxacin; resistance to carbapenems, piperacillin/tazobactam, gentamicin, and tobramycin was noted in more than 80% of strains. A comparison of two periods showed that the percentages of A. baumannii strains producing two or three types of β-lactamases were significantly greater in 2021–2022 than in 2016–2017 (94.2% and 5.8% vs. 17.7% and 2.3%, respectively, p < 0.001). Isolates producing two or three types of β-lactamases were more often resistant to tigecycline, tetracycline, and doxycycline than strains producing one type of β-lactamase (p < 0.001). Conclusions: The frequency of isolation of A. baumannii strains producing two different types of β-lactamases (AmpC plus KPC, AmpC plus ESBL, or ESBL plus KPC) or three types of β-lactamases (AmpC, KPC, and ESBL) and the resistance rates to ampicillin/sulbactam, tigecycline, tetracycline, and doxycycline were significantly greater in 2020–2021 as compared with 2016–2017. The production of two or three types of β-lactamases by A. baumannii strains was associated with higher resistance rates to tetracyclines.

show abstract

“…The prevalence of A. baumannii was 21.11% (19/90) in this study which is similar to some previous studies reported from Nepal [36,37] . As the pathogen can endure dry conditions for a long time, they persist in the hospital environment and are responsible for opportunistic infections [17] . Hence, the highest number of isolates were isolated from in-patients (84.2%) which is following other studies [38,49] .…”

Section: Discussionmentioning

confidence: 99%

“…The prevalence of carbapenem resistance in Acinetobacter in UK, USA, Singapore, Chile, Korea, Portugal, India, and Pakistan was 47-77%, 48%, 50%, 70%, 92%, 85%, 40% and 62-100%, respectively [41][42][43][44][45][46] . The development of resistance in A. baumannii may be due to the presence of wide array 𝛽 -lactamases that hydrolyze and confer resistance to penicillins, cephalosporins, and carbapenems [17] . Resistance to carbapenem is facilitated by resistance mechanisms including enzymatic inactivation, active efflux of drugs, and modification of target sites [47] .…”

Section: Discussionmentioning

confidence: 99%

“…This study showed that all the isolates contained blaOXA-23 whereas blaOXA-24 and blaOXA-58 were not detected in any of the analyzed isolates [13] . Besides, previous studies have reported varying prevalence of carbapenem resistance in bacteria in different settings, including 9.09% in Shahid Gangalal National Heart Centre, Kathmandu [14] , 25.7% in Nepal Medical College, Kathmandu [15] , 56% in Shree Birendra Hospital, Kathmandu [16] , and 28% in Paropakar Maternity and Women's Hospital, Kathmandu [17] . The prevalence of carbapenem-resistant strains varies in different hospitals, laboratories, and research centers among the samples used for the isolation of bacteria.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal

Ghimire

Kandel

Neupane³

et al. 2021

Prog Micobes Mol Biol

Self Cite

View full text Add to dashboard Cite

(1) Background: Acinetobacter baumannii has emerged as a leading cause of nosocomial infections as they are capable of evolving resistance to various classes of antibiotics. The ability of A. baumannii to form biofilm might also be associated with increased antibiotic resistance and hence treatment failure. This study was carried to associate the biofilm formation with the drug resistance pattern of A. baumannii and to detect blaOXA-23, blaOXA-24, and blaOXA-51 from carbapenem resistance isolates. (2) Methods: Among different clinical samples, a total of 19 Acinetobacter spp. were identified with conventional microbiological procedures. The biofilm production was determined by a quantitative adherence assay. The antimicrobial susceptibility test was carried out by the Kirby-Bauer disc diffusion method, carbapenemase production detection was confirmed by Modified Hodge Test. And target resistant genes were detected by polymerase chain reaction. (3) Results: Out of 90 clinical specimens, 64.44% (58/90) showed bacterial growth. Whereas, 32.75% (19/58) isolates were identified as A. baumannii. Among all A. baumannii isolates, 84.21% (16/19) were multidrug-resistance and 63.16% (12/19) carbapenem resistance phenotypically. blaOXA-51 was detected in all the isolates and blaOXA-23 was detected only in 63.16% (12/19) isolates. However, blaOXA-24 was not detected in any of the isolates. Among A. baumannii, 89.47% (17/19) isolates produced biofilm with 47.37% (9/19) strong biofilm producers. (4) Conclusions: In the majority of MDR A. baumannii, blaOXA-51 and blaOXA-23 were detected as the determinant factor for carbapenem resistance having a direct relation with biofilm formation. This study provided a valuable clue for the management of A. baumannii infections in clinical settings.

show abstract

Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens

Cited by 3 publications

References 13 publications

Associations Between β-Lactamase Types of Acinetobacter Baumannii and Antimicrobial Resistance

Associations Between β-Lactamase Types of Acinetobacter Baumannii and Antimicrobial Resistance

Associations between β-Lactamase Types of Acinetobacter baumannii and Antimicrobial Resistance

Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal

Contact Info

Product

Resources

About