Beta-trace protein as sensitive marker for CSF rhinorhea and CSF otorhea

Reiber, Hansotto; Walther, K.; Althaus, Harald

doi:10.1034/j.1600-0404.2003.00173.x

Cited by 54 publications

(43 citation statements)

References 12 publications

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“…b-trace protein (prostaglandin D synthase) is an abundant brain-specific protein, comprising about 3% of the total CSF protein [5]. It is synthesised by both the choroid plexus and the leptomeninges [4], and is normally found in CSF in concentrations 35 times higher than in serum. This ratio is the highest of all the CSF-specific proteins, making b-trace protein an ideal marker for the detection of CSF [6,7].…”

Section: Discussionmentioning

confidence: 99%

“…For determination of b-trace protein concentration, the samples were batched, stored at )20°C and batch assayed using the nephelometric assay (Dade Behring, Liederbach, Germany). This assay has been described in detail elsewhere [4,9,10]. In brief, it is an immunonephelometric assay based on latex particle enhancement using rabbit polyclonal antibodies against b-trace protein.…”

Section: Methodsmentioning

confidence: 99%

“…A sample size of at least 200 ll is preferred, although samples as small as 20 ll may be adequate with careful manual predilution. The assay takes about 20 min [4]. Means, standard deviations and confidence intervals (CI) for the mean were calculated for normally distributed values; otherwise, median, lower and upper quartiles (Q1 and Q3) and range were calculated.…”

Section: Methodsmentioning

confidence: 99%

“…A fast and accurate test to detect CSF in such fluid would be valuable, given concern that meningitis might complicate the clinical picture [3]. The nephelometric assay of b-trace protein is considered the best method of detecting CSF rhinorrhoea and otorrhoea [4], and concentrations of serum and CSF b-trace protein have been determined for healthy male and female patients. However, the CSF concentration of b-trace protein has not been studied in the pregnant population.…”

mentioning

confidence: 99%

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Cerebrospinal fluid and serum concentrations of beta‐trace protein during pregnancy

McArthur

Hill²,

Paech

et al. 2005

Anaesthesia

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SummaryWe conducted a prospective observational study among a cohort of 40 term parturients undergoing spinal anaesthesia for elective Caesarean section, to determine the concentration of b-trace protein in cerebrospinal fluid (CSF) and serum. Serum and CSF samples, taken at the time of dural puncture, were assayed by nephelometry. The mean serum b-trace protein concentration was 0.39 mg.l )1 and the mean CSF concentration was 27.9 mg.l )1 , giving a mean ratio of CSF to serum concentration of 76. This ratio is higher than that published for non-pregnant females and for males because of both a higher mean CSF and a lower mean serum b-trace protein concentration. The concentration correlated positively with both serum creatinine and gestational age. If these concentrations are used to estimate the normal range, we propose that the nephelometric measurement of b-trace protein might prove a useful diagnostic test for cerebrospinal fluidcutaneous fistula in parturients. Reports of cerebrospinal fluid-cutaneous fistulae (CSFCF) complicating epidural anaesthesia are uncommon [1]. However, in our experience, fluid leakage at the site of epidural needle insertion for combined spinal-epidural anaesthesia is not uncommon [2]. A fast and accurate test to detect CSF in such fluid would be valuable, given concern that meningitis might complicate the clinical picture [3]. The nephelometric assay of b-trace protein is considered the best method of detecting CSF rhinorrhoea and otorrhoea [4], and concentrations of serum and CSF b-trace protein have been determined for healthy male and female patients. However, the CSF concentration of b-trace protein has not been studied in the pregnant population. b-trace protein (prostaglandin D synthase) is an abundant brain-specific protein, comprising about 3% of the total CSF protein [5]. It is synthesised by both the choroid plexus and the leptomeninges [4], and is normally found in CSF in concentrations 35 times higher than in serum. This ratio is the highest of all the CSF-specific proteins, making b-trace protein an ideal marker for the detection of CSF [6,7].

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Cerebrospinal fluid and serum concentrations of beta‐trace protein during pregnancy

McArthur

Hill²,

Paech

et al. 2005

Anaesthesia

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“…1998; Reiber et al. 2003; Mase et al. 2003), as found in four of the five samples from the patients evaluated here, support this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Levels of beta‐trace protein in optic disc pit with macular detachment

Makdoumi

Nilsson

Crafoord

2017

Acta Ophthalmologica

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BackgroundTo report beta‐trace protein (β TP) levels in the subretinal fluid (SRF) of four patients with a macular detachment associated with optic disc pit (ODP).MethodsFour patients with a serous retinal detachment involving the macula was operated by pars plana vitrectomy (PPV) with C2F6 gas tamponade and peeling of internal limiting membrane (ILM). Patients with a follow‐up period exceeding one year postoperatively were included in the study. The SRF was drained using a fine cannula without laser photocoagulation, and the samples were analysed using particle‐enhancing nephelometry. The levels of β TP were compared to 20 routine cerebrospinal fluid (CSF) samples.ResultsIn four of the five samples from SRF had relatively low β TP levels, with a mean concentration of 6.6 mg/l (range 2.0 to 23.1 mg/l) compared to 16.0 mg/l (range 6.3‐26.8 mg/l) in CSF. The only SRF sample within the range corresponding to normal CSF was the first sample from patient 4, and the analysis of the renewed aspirate during the second operation was 2.8 mg/l. Postoperatively, the regression of SRF was slow, but regression of SRF in the foveal region took place in all cases; however, visual acuity (VA) was improved in only half of the patients.ConclusionThe results from the analysed SRF regarding β TP concentration in these patients indicate that the SRF in ODP is not identical to CSF, as the concentrations of β TP differ.

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