Glass/SU-8 microchip for electrokinetic applicationsIn this communication, we describe the fabrication and electric characterization of a hybrid glass/SU-8 microchannels for high-performance electrokinetic applications. The bonding process employed SU-8 film as intermediate layer with reduced baking times; all the procedure took less than 50 min (only about 10 min disregarding the cleaning and dehydration steps). Additionally, further steps to improve the adhesion of the substrate to the SU-8 were not needed. The developed configuration aggregates the advantages of both substrates, including (i) simple fabrication techniques; (ii) high compatibility for integration of microelectromechanical, optical, and electrochemical components (SU-8); (iii) high and stable electroosmotic mobility ( EO ); and (iv) satisfactory heat dissipation capacity (glass). Electroosmotic mobilities were measured as a function of the pH using the current monitoring method, whereas the heat dissipation capacity was investigated through Ohm's law plots for both glass and glass/SU-8 microchips. The measured EO values were similar for both microdevices, with mobilities of the order of 4.0-4.5 × 10 −4 cm 2 V −1 cm −1 at 4-12 pH range using phosphate buffer (10 and 20 mmol/L). The heat dissipation assays were carried out in microchannels filled with 20 mmol/L phosphate buffer. A considerable Joule heating was observed only at electric field strengths greater than 580 V cm −1 in hybrid glass/SU-8 microdevices, representing a substantial increase of 48% when compared to all SU-8 microdevices.
Keywords:Bonding / Microfabrication / Photoresist DOI 10.1002/elps.201300167Additional supporting information may be found in the online version of this article at the publisher's web-siteMicrodevices integrating CE are a potential platform for microfluidics applications, especially for biomolecular assays in proteomics and metabolomics research. This platform provides simple, effective, small-in-volume, and high-resolution separations. Glass is the most used material in the fabrication of CE chips [1]. Vitreous substrates carry important advantages, including (i) optical transparency, (ii) chemical inertia, (iii) high and stable electroosmotic mobility ( EO ), and (iv) good thermal conductivity ( = 1.5 W mK −1 ) [2]. The latter allows the application of high electric field strengths (>600 V cm −1 ) without formation of temperature gradienta negligible Joule heating, contributing for fast separations.