Beyond Genome Editing: CRISPR Approaches

Sultan, Qaisar; Ashraf, Sidra; Munir, Ahmad; Khan, Sultan Habibullah; Munawar, Nayla; Abd‐Elsalam, Kamel A.; Ahmad, Aftab

doi:10.1007/978-981-16-6305-5_6

Cited by 2 publications

(1 citation statement)

References 144 publications

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“…By combining it with transcriptional activators, specific genes can be induced, resulting in their activation. This technique holds great potential in facilitating the activation of tumor suppressor genes ( Sultan et al, 2022 ).…”

Section: Crispr/cas12mentioning

confidence: 99%

CRISPR/Cas9 as a therapeutic tool for triple negative breast cancer: from bench to clinics

Tiwari

Dubey

et al. 2023

Front. Mol. Biosci.

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Clustered regularly interspaced short palindromic repeats (CRISPR) is a third-generation genome editing method that has revolutionized the world with its high throughput results. It has been used in the treatment of various biological diseases and infections. Various bacteria and other prokaryotes such as archaea also have CRISPR/Cas9 systems to guard themselves against bacteriophage. Reportedly, CRISPR/Cas9-based strategy may inhibit the growth and development of triple-negative breast cancer (TNBC) via targeting the potentially altered resistance genes, transcription, and epigenetic regulation. These therapeutic activities could help with the complex issues such as drug resistance which is observed even in TNBC. Currently, various methods have been utilized for the delivery of CRISPR/Cas9 into the targeted cell such as physical (microinjection, electroporation, and hydrodynamic mode), viral (adeno-associated virus and lentivirus), and non-viral (liposomes and lipid nano-particles). Although different models have been developed to investigate the molecular causes of TNBC, but the lack of sensitive and targeted delivery methods for in-vivo genome editing tools limits their clinical application. Therefore, based on the available evidences, this review comprehensively highlighted the advancement, challenges limitations, and prospects of CRISPR/Cas9 for the treatment of TNBC. We also underscored how integrating artificial intelligence and machine learning could improve CRISPR/Cas9 strategies in TNBC therapy.

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Section: Crispr/cas12mentioning

confidence: 99%

CRISPR/Cas9 as a therapeutic tool for triple negative breast cancer: from bench to clinics

Tiwari

Dubey

et al. 2023

Front. Mol. Biosci.

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Genome editing in cotton: challenges and opportunities

Khan

Ahmed

et al. 2023

J Cotton Res

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Cotton has enormous economic potential providing high-quality protein, oil, and fibre. A large increase in cotton output is necessary due to the world's changing climate and constantly expanding human population. In the past, conventional breeding techniques were used to introduce genes into superior cotton cultivars to increase production and to improve quality. The disadvantages of traditional breeding techniques are their time-consuming, reliance on genetic differences that are already present, and considerable backcrossing. To accomplish goals in a short amount of time, contemporary plant breeding techniques, in particular modern genome editing technologies (GETs), can be used. Numerous crop improvement initiatives have made use of GETs, such as zinc-finger nucleases, transcription-activator-like effector nucleases, clustered regularly interspaced palindromic repeats (CRISPR), and CRISPR-associated proteins systems (CRISPR/Cas)-based technologies. The CRISPR/Cas system has a lot of potential because it combines three qualities that other GETs lack: simplicity, competence, and adaptability. The CRISPR/Cas mechanism can be used to improve cotton tolerance to biotic and abiotic stresses, alter gene expression, and stack genes for critical features with little possibility of segregation. The transgene clean strategy improves CRISPR acceptability addressing regulatory issues associated with the genetically modified organisms (GMOs). The research opportunities for using the CRISPR/Cas system to address biotic and abiotic stresses, fibre quality, plant architecture and blooming, epigenetic changes, and gene stacking for commercially significant traits are highlighted in this article. Furthermore, challenges to use of CRISPR technology in cotton and its potential for the future are covered in detail.

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Beyond Genome Editing: CRISPR Approaches

Cited by 2 publications

References 144 publications

CRISPR/Cas9 as a therapeutic tool for triple negative breast cancer: from bench to clinics

CRISPR/Cas9 as a therapeutic tool for triple negative breast cancer: from bench to clinics

Genome editing in cotton: challenges and opportunities

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