Bi-enzyme synergetic catalysis to in situ generate coreactant of peroxydisulfate solution for ultrasensitive electrochemiluminescence immunoassay

Recent Advances in Electrochemical Biosensors Based on Fullerene‐C60 Nano‐structured Platforms

“…A peroxydisulfate/oxygen (S2O8 2− /O2) system is widely used for amplification of ECL signals where the dissolved O2 can serve as a co-reactant [67]. The enzymatic reaction can catalyze in situ generation of the dissolved O2 [63,65].…”

Self-enhanced electrochemiluminescence immunosensor based on nanowires obtained by a green approach

“…Prior to modification, the glassy carbon electrode (GCE) (Ф = 4 mm) was polished with 0.3, 0.05 μm alumina slurry respectively, followed by rinsing thoroughly with bi-distilled water and sonicateing in ethanol, bi-distilled water separately. The cleaned GCE was firstly immersed into HAuCl 4 (1%) and electrodeposited with constant potential -0.2 V for 30 s to obtain AuNPs layer(Wang et al, 2012;Wang et al, 2013). Then, 18 μL capture antibody (Ab 1 ) was dropped onto the electrode followed by incubating at 4 • C for 12 h. Then, 16 μL of BSA (1%) solution was placed onto the electrode for 40 min at room temperature to block the remaining active sites.…”

Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

“…However, CS has as main disadvantage to act as an insulator, which hinders the charge-transfer process. In order to improve the current signal, the enrichment of CS matrix with metallic nanoparticles has shown interesting results [21,24,29,33]. Graphene shows great promise for the development of electrochemical biosensors due to its excellent mechanical flexibility, fast electron transfer, and good biocompatibility [13,[34][35][36].…”

“…Enzyme selection and their sources have a major influence on the biosensor sensitivity [19,22]. The few studies dedicated to bi-enzymatic biosensors [17][18][19][20][21][23][24][25] reported in the last ten years are summarized in Table 1S (Supplementary material). As far as the authors know, there is no publication related to the application of bi-enzymatic biosensors for the quantification of pesticides in food commodities or in other real samples.…”

“…Due to the presence of amino and hydroxyl groups, CS film exhibits multiple functionalities. CS can be cross-linked with nanomaterials [9,20,24,29], inorganic complexes [30], and biological elements [20,31], and used as support for blends with other polymers [32]. CS has excellent membrane-forming ability, high permeability towards water, good adhesion and biocompatibility providing a suitable microenvironment for electroimmobilization of biomolecules on different working surfaces [14,29,33].…”