“Bicellar” Lipid Mixtures as used in Biochemical and Biophysical Studies

Katsaras, John; Harroun, Thad A.; Pencer, Jeremy; Nieh, Mu‐Ping

doi:10.1007/s00114-005-0641-1

Cited by 118 publications

(166 citation statements)

References 97 publications

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“…4a] and ULVs at 50 8C [ Fig. 4b], are consistent with previous reports [21][22][23]. Best fits of bicelle data (i.e., at 10 8C) resulted in radii of 173, 187, and 214 Å , for [Chol]/[DMPC] = 0, 10%, and 20%, respectively, and a bilayer thickness *44 Å .…”

Section: Membrane Rigiditysupporting

confidence: 90%

“…Previous SANS data obtained at low temperatures (<20 8C) have indicated that the lipid mixtures in question self-aggregate into bicelles, with the long-chain lipids (e.g., DMPC, DMPG) forming the extended bilayered plane, and most of the short-chain lipid (i.e., DHPC) sequestering into the bicelle's high-curvature rim [21][22][23], as shown in Fig. 2.…”

Section: Resultsmentioning

confidence: 83%

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Formation mechanism of self-assembled unilamellar vesiclesSpecial issue on Neutron Scattering in Canada

2010

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Uniform size self-assembled unilamellar vesicles (ULVs) can be produced from mixtures of weakly charged short-and long-chain phospholipids. These lipid mixtures self-assemble into bilayered micelles (so-called bicelles), and a bicelle to ULV transition has been previously reported. Here, we discuss the effect of various parameters (i.e., lipid concentration, charge density, membrane rigidity, lipid composition, and lipid hydrocarbon chain length) on ULV radius as determined by small angle neutron scattering (SANS). SANS data were best fit using a core-shell disk and a spherical-shell model to obtain the size of bicelles and ULVs, respectively. From the present experiments we conclude that a previously proposed mechanism of ULV formation, where bicelles coalesce into large precursor and self-fold into ULVs, is able to explain the present SANS data.PACS No: 87.14.Cc Résumé : Il est possible de produire des vésicules à une couche auto-assemblées et de grosseur uniforme (ULVs), à partir de mélanges de chaînes de phospholipides courtes et longues faiblement chargées. Ces mélanges de lipides s'auto-assemblent en micelles bicouches (parfois appelées bicelles) et une transition de bicelle à ULV a déjà été décrite. Nous discutons ici l'effet de différents paramètres (la concentration lipidique, la densité de charge, la rigidité de membrane, la composition lipidique et la longueur des chaînes lipidiques) sur le rayon des ULV qui est déterminé par diffusion de neutrons aux petits angles (SANS). Nous ajustons la courbe des données SANS à l'aide d'un modèle en couches sphériques pour obtenir la grosseur des bicelles et des ULV respectivement. À partir des présentes expériences, nous concluons qu'un mécanisme préalablement rapporté sur la formation des ULV, où les bicelles coalescent en un gros précurseur et s'auto-replient en ULV, est capable d'expliquer les présentes données SANS.[Traduit par la Rédaction]

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Section: Membrane Rigiditysupporting

confidence: 90%

Section: Resultsmentioning

confidence: 83%

Formation mechanism of self-assembled unilamellar vesiclesSpecial issue on Neutron Scattering in Canada

2010

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“…For temperatures above the gel-fluid transition, AmB slightly increased the bilayer fluidity, which was already fluid . The preferential binding of AmB to the highly disorganized edges of DODAB bicelles suggests that the same strategy could be employed for other formulations, such as mixtures of natural lipids known to form bicelles (Katsaras et al 2005).…”

Section: Detecting Dodab Bicelles By Esrmentioning

confidence: 99%

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

et al. 2017

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Cationic bilayers have been used as models to study membrane fusion, templates for polymerization and deposition of materials, carriers of nucleic acids and hydrophobic drugs, microbicidal agents and vaccine adjuvants. The versatility of these membranes depends on their structure. Electron spin resonance (ESR) spectroscopy is a powerful technique that employs hydrophobic spin labels to probe membrane structure and packing. The focus of this review is the extensive structural characterization of cationic membranes prepared with dioctadecyldimethylammonium bromide or diC14-amidine to illustrate how ESR spectroscopy can provide important structural information on bilayer thermotropic behavior, gel and fluid phases, phase coexistence, presence of bilayer interdigitation, membrane fusion and interactions with other biologically relevant molecules.

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“…DMPG has the same hydrocarbon chains as those of DMPC, and is known to stabilize bicelles through electrostatic repulsion, which inhibits bicelles from fusing, as well as increasing membrane rigidity. 20 A previous report showed a non DMPG doped system ([DMPG]/[DMPC] ) 0) formed MLVs due to insufficient Coulombic repulsion between bilayers, while a highly doped system ([DMPG]/ [DMPC] ) 6.7%) formed bicelles which were unable to fuse and form ULVs at T > T c . 21 The present study focuses on the kinetics of aggregate structures that evolve from the bicelle-to-ULV transition under different temperature protocols as determined by small angle neutron scattering (SANS).…”

Section: Introductionmentioning

confidence: 99%

Effects of Charge Density and Thermal History on the Morphologies of Spontaneously Formed Unilamellar Vesicles

et al. 2010

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“Bicellar” Lipid Mixtures as used in Biochemical and Biophysical Studies

Cited by 118 publications

References 97 publications

Formation mechanism of self-assembled unilamellar vesiclesSpecial issue on Neutron Scattering in Canada

Formation mechanism of self-assembled unilamellar vesiclesSpecial issue on Neutron Scattering in Canada

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

Effects of Charge Density and Thermal History on the Morphologies of Spontaneously Formed Unilamellar Vesicles

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