Bidentate acylthiourea ligand anchored Pd-PPh3 complexes with biomolecular binding, cytotoxic, antioxidant and antihemolytic properties

Dorairaj, Dorothy Priyanka; Haribabu, Jebiti; Shashankh, Penki V.S.; Chang, Yu‐Lun; Echeverría, César; Hsu, Sodio C. N.; Karvembu, Ramasamy

doi:10.1016/j.jinorgbio.2022.111843

Cited by 15 publications

(14 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Control cells generally emit a green fluorescence while a change from green to yellow indicates early apoptosis. Late apoptosis is characterized by the transformation from yellow to orange and lastly, a red fluorescence indicates necrosis 48 . From Figure 8a, we could infer that upon the dose‐dependent concentration of Cu‐3 at 25, 50, and 75 μM, visible change in the fluorescence color from green to yellow and finally orange was seen.…”

Section: Resultsmentioning

confidence: 94%

“…Late apoptosis is characterized by the transformation from yellow to orange and lastly, a red fluorescence indicates necrosis. 48 From Figure 8a, we could infer that upon the dose-dependent concentration of Cu-3 at 25, 50, and 75 μM, visible change in the fluorescence color from green to yellow and finally orange was seen. Hence, it was understood that Cu-3 induced late apoptosis in HeLa cells.…”

Section: Apoptotic Mode Of Cell Death In Hela Cells By Cu-3mentioning

confidence: 91%

See 1 more Smart Citation

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Narwane

Dorairaj

Roymahapatra

et al. 2023

Applied Organom Chemis

Self Cite

View full text Add to dashboard Cite

Four copper (II) complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate (Tppy) ligand with corresponding chloride (Cu‐1), aqua (Cu‐2), azide (Cu‐3), and thiocyanide (Cu‐4) substitutions were synthesized and characterized by spectroscopic and analytical methods. Spectroscopic and molecular docking studies were employed to investigate the interactions of these complexes with calf thymus (CT) DNA and bovine serum albumin (BSA). The results inferred intercalation binding mode of the complexes with DNA. All the complexes exhibited good binding with BSA as well. In addition, the binding efficacy of the Cu (II) complexes with SARS‐Cov‐2 was tested in silico. Further, in vitro anticancer activity of the complexes was investigated against the HeLa‐cervical, HepG2‐liver and A549‐lung cancer, and one normal (L929‐fibroblast) cell line. IC50 values unveiled that the complexes were more active than cisplatin against all three cancer cells. It was understood that complex Cu‐3 containing azide substitution displayed the highest activity on the HeLa cell line (IC50 = 6.3 μM). More importantly, TppyCu (II) complexes were not active against the normal cell line. Lastly, the acridine orange/ethidium bromide (AO/EB) and 4′,6‐diamidino‐2‐phenylindole staining assays indicated that Cu‐3 induced cell death in HeLa cells at the late apoptotic stage. This complex also efficiently generated ROS in HeLa cells promoting apoptosis as understood from the DCFH‐DA assay.

show abstract

Section: Resultsmentioning

confidence: 94%

Section: Apoptotic Mode Of Cell Death In Hela Cells By Cu-3mentioning

confidence: 91%

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Narwane

Dorairaj

Roymahapatra

et al. 2023

Applied Organom Chemis

Self Cite

View full text Add to dashboard Cite

show abstract

“…BSA has two active sites, namely, site‐I and site‐II, and two tryptophan residues (Trp‐134 and Trp‐212) 28 . The fluorescence spectra were recorded in the range of 290–500 nm upon an excitation at 280 nm.…”

Section: Resultsmentioning

confidence: 99%

“…BSA has two active sites, namely, site-I and site-II, and two tryptophan residues (Trp-134 and Trp-212). 28 The fluorescence spectra were recorded in the range of 290-500 nm upon an excitation at 280 nm. When an increasing amount of complex (0-14 μM) was added to a fixed quantity of BSA (1 μM), a quenching pattern was observed at 342 nm accompanied by a minor hypsochromic shift (73.7 [C1], 74.9 [C2], 76.3 [C3], 80.2 [C4], and 78.2% [C5]) as seen in Figures 5b and S30.…”

Section: Binding Of the Cu(i) Complexes With Bovine Serum Albumin (Bsa)mentioning

confidence: 99%

Anti‐proliferative potential of copper(I) acylthiourea complexes with triphenylphosphine against breast cancer cells

Dorairaj

Haribabu

Mahendiran

et al. 2023

Applied Organom Chemis

Self Cite

View full text Add to dashboard Cite

We report the synthesis of five new Cu(I) acylthiourea complexes (C1–C5) bearing the general formula [Cu(L‐R)Cl (PPh3)2] [L = monodentate acylthiourea ligand, R = C6H5 (L1), C6H4CH3(o) (L2), C6H4OCH2CH3(p) (L3), C10H7 (L4) or C6H4Cl(p) (L5)]. All the complexes were characterized by analytical and spectroscopic tools. The complexes (C1–C5) exhibited a distorted tetrahedral geometry as inferred from the single crystal X‐ray diffraction study. The complexes were subjected to interact with the biomolecules (calf thymus [CT] DNA/bovine serum albumin [BSA]); the one bearing naphthyl substituent (C4) exhibited the highest binding efficacy. Further, anticancer activity of the complexes was studied exclusively against breast cancer cell lines, namely, MCF7, T47D, and MDA MB 231. Complex C4 was found to be highly cytotoxic on the three cancer cell lines with the IC50 values of 0.75, 0.75, and 0.68 μM, respectively. Conveniently, the complexes displayed fourfold less toxicity against the normal MCF10a human breast cells. The ability of complex C4 to induce apoptosis was analyzed by acridine orange/ethidium bromide (AO/EB) and Hoechst 33258 staining assays. Furthermore, it was found that complex C4 induced apoptosis via reactive oxygen species (ROS)‐mediated mitochondrial signaling pathway. Confocal fluorescence images of the cells subjected to lyso and mitotracker staining assays revealed that complex C4 was primarily localized on the mitochondria, and finally, Western blot analysis also confirmed the apoptosis induced by complex C4 in the MDA MB 231 cancer cells.

show abstract

“…Noteworthily, new sharp bands corresponding to Pd-C band has developed around 505-508 cm −1 , and distinctive Pd-PPh 3 absorption signals has emerged around 1445-1451 cm −1 in the spectra of Pd1-Pd3. 43,44 FT-IR spectra demonstrated the tridentate binding fashion of thiourea to Pd via the aryl carbon-ion, hydrazinic nitrogen atom and thiocarbonyl sulfur atom.…”

Section: Resultsmentioning

confidence: 99%

Orthometallated Pd(ii) C^N^S pincer complex catalyzed sustainable synthesis of bis(indolyl)methanesviaacceptorless dehydrogenative coupling of alcohols

Selvan

Rengan

Malecki

2023

Catal. Sci. Technol.

View full text Add to dashboard Cite

show abstract

Bidentate acylthiourea ligand anchored Pd-PPh3 complexes with biomolecular binding, cytotoxic, antioxidant and antihemolytic properties

Cited by 15 publications

References 53 publications

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Synthesis, characterization, cytotoxicity evaluation, and molecular docking with DNA, bovine serum albumin, and SARS‐Cov‐2 of copper complexes bearing tris‐(2‐pyridyl)‐pyrazolyl borate ligand

Anti‐proliferative potential of copper(I) acylthiourea complexes with triphenylphosphine against breast cancer cells

Orthometallated Pd(ii) C^N^S pincer complex catalyzed sustainable synthesis of bis(indolyl)methanesviaacceptorless dehydrogenative coupling of alcohols

Contact Info

Product

Resources

About