Bifidobacterium BLa80 mitigates colitis by altering gut microbiota and alleviating inflammation

Dong, Yao; Liao, Wenyan; Tang, Jing; Teng, Fei; Gai, Zhonghui; Han, Mei

doi:10.1186/s13568-022-01411-z

Cited by 34 publications

(19 citation statements)

References 47 publications

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“…DNA was isolated from the samples for testing using the QIAamp DNA kit according to the manufacturer’s instructions. Polymerase chain reaction was used to amplify the V3–V4 variable region of the prokaryotic 16S rRNA gene as previously described ( 20 ) using the primers F1 and R2 (5′- CCTACGGGNGGCWGCAG-3′ and 5′-GACTACHVGGGTATCTAATCC-3′). Sequencing was performed on the Miseq platform (Illumina, San Diego, CA, USA) using a 2 × 300 bp paired-end protocol.…”

Section: Methodsmentioning

confidence: 99%

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Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study

Gai¹,

Dong²,

Xu³

et al. 2023

Front. Nutr.

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The gut microbiota promotes gastrointestinal health in humans; however, the effect of probiotics on the gut microbiota of healthy adults has not been documented clearly. This placebo-controlled study was conducted to assess the effect of Lacticaseibacillus rhamnosus LRa05 supplementation on the gut microbiota of healthy adults. The subjects (N = 100) were randomized 1:1 to receive (1) maltodextrin (placebo, CTL group) and (2) maltodextrin + strain LRa05 (1 × 1010 colony-forming units/day, LRa05 group). The duration of the intervention was 4 weeks, and changes in the gut microbiota from before to after the intervention were investigated using 16S rRNA high-throughput sequencing. In terms of alpha diversity, no significant difference in the composition of the gut microbiota was found between the LRa05 and CTL groups. 16S rRNA sequencing analysis showed that the relative abundance of Lacticaseibacillus significantly increased after supplementation with LRa05. Furthermore, a decreasing trend in the abundance of Sellimonas and a significant decrease in the salmonella infection pathway were observed in the LRa05 group compared with the CTL group. These findings indicate the potential of LRa05 to colonize the human gut and reduce the abundance of harmful bacteria in the microbiota.

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Section: Methodsmentioning

confidence: 99%

“…Methods for bioinformatics analysis of amplicons were adopted from our previous publications ( 20 , 21 ). Trimmomatic was used to filter low quality sequences ( 22 ).…”

Section: Methodsmentioning

confidence: 99%

Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study

Gai¹,

Dong²,

Xu³

et al. 2023

Front. Nutr.

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“…Extraction of fecal DNA and amplification of the V3–V4 region of 16S rRNA was performed as previously reported ( 15 , 18 ). Purified PCR products were sequenced using the Illumina MiSeq PE300 platform (Illumina, USA).…”

Section: Methodsmentioning

confidence: 99%

Lacticaseibacillus rhamnosus Hao9 exerts antidiabetic effects by regulating gut microbiome, glucagon metabolism, and insulin levels in type 2 diabetic mice

Han

Liao

Dong³

et al. 2023

Front. Nutr.

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IntroductionType 2 diabetes mellitus (T2DM) is a metabolic disease that has led to a significant global public health burden.MethodsIn this work, we investigated the effects of Lacticaseibacillus rhamnosus Hao9 on T2DM in mice with high-fat diet- and streptozotocin (STZ)-induced diabetes (diabetic mice) and explored the underlying mechanisms.ResultsWe found that 109 colony forming units (CFUs) of Hao9 per day significantly reduced fasting blood glucose and insulin levels (p < 0.001) in diabetic mice. Moreover, Hao9 enhanced liver antioxidant capacity and significantly decreased glucose-6-phosphatase and phosphoenolpyruvate carboxykinase expression in the livers of diabetic mice (p < 0.001). Hao9 also reduced the serum concentrations of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNFα), interleukin-1β (IL1β), and IL6 (p < 0.05) and improved intestinal barrier function in diabetic mice. The composition of the gut microbiome was modulated by Hao9, with an increased abundance of Roseburia, Eubacterium, and Lacticaseibacillus, and decreased abundance of Escherichia/Shigella. Notably, Lacticaseibacillus was detected at both weeks 5 and 12 post-treatment, suggesting sustained colonization of the gut by Hao9.DiscussionThe supplementation of Hao9 improved gut microbiota, glucose metabolism, and insulin levels significantly in T2DM mice. That means Hao9 contributes to improving T2DM symptoms with its potential beneficial effects. Therefore, Hao9 is a promising dietary supplement for the treatment of T2DM.

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“…Bifidobacterium lactis’ role is important in ameliorating the pro-inflammatory processes of the intestine, mainly contributing to the reduction in factors such as IFN-γ, IL-1β, IL-6, IL-8, IL-17 and TNF-α [ 26 , 27 , 28 , 29 , 30 , 31 ]. Huang et al and Turner et al showed that pretreatment with this strain can also benefit the innate immunity responses towards pathogen infections [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

The Probiotic Strains Bifidοbacterium lactis, Lactobacillus acidophilus, Lactiplantibacillus plantarum and Saccharomyces boulardii Regulate Wound Healing and Chemokine Responses in Human Intestinal Subepithelial Myofibroblasts

et al. 2022

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Bifidobacterium lactis, Lactobacillus acidophilus, Lactiplantibacillus plantarum and Saccharomyces boulardii are common probiotic supplements. Colonic subepithelial myofibroblasts (cSEMFs) are actively involved in mucosal wound healing and inflammation. cSEMFs, isolated from healthy individuals, were stimulated with 102 or 104 cfu/mL of these probiotic strains alone and in combination, and their effect on chemokine and wound healing factor expression was assessed by qRT-PCR, ELISA and Sircol Assay, and on cSEMFs migration, by Wound Healing Assay. These strains remained viable and altered cSEMFs’ inflammatory and wound healing behavior, depending on the strain and concentration. cSEMFs treated with a combination of the four probiotics had a moderate, but statistically significant, increase in the mRNA and/or protein expression of chemokines CXCL1, CXCL2, CXCL4, CXCL8, CXCL10, CCL2 and CCL5, and healing factors, collagen type I and III, fibronectin and tissue factor. In contrast, when each strain was administered alone, different effects were observed, with greater increase or decrease in chemokine and healing factor expression, which was balanced by the mixture. Overall, this study highlights that the use of multiple probiotic strains can potentially alert the gut mucosal immune system and promote wound healing, having a better effect on mucosal immunity than the use of single probiotics.

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Bifidobacterium BLa80 mitigates colitis by altering gut microbiota and alleviating inflammation

Cited by 34 publications

References 47 publications

Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study

Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study

Lacticaseibacillus rhamnosus Hao9 exerts antidiabetic effects by regulating gut microbiome, glucagon metabolism, and insulin levels in type 2 diabetic mice

The Probiotic Strains Bifidοbacterium lactis, Lactobacillus acidophilus, Lactiplantibacillus plantarum and Saccharomyces boulardii Regulate Wound Healing and Chemokine Responses in Human Intestinal Subepithelial Myofibroblasts

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