Bifidobacterium longum IM55 and Lactobacillus plantarum IM76 alleviate allergic rhinitis in mice by restoring Th2/Treg imbalance and gut microbiota disturbance

Kim, W.-G.; Kang, Geum-Dan; Kim, H.I.; Han, Myung Joo; Kim, D.-H.

doi:10.3920/bm2017.0146

Cited by 59 publications

(47 citation statements)

References 56 publications

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“…studies reporting positive effects of administration with various probiotic Bifidobacterium spp. on the Treg/T effector cell balance in humans (Konieczna et al, 2012) and mice, which thereby ameliorate allergic and autoimmune diseases (Lyons et al, 2010;Zuo et al, 2014;Liu et al, 2018;Kim et al, 2019). Furthermore, a growing body of evidence suggests an important immunomodulatory role of hexa-acylated lipopolysaccharide (LPS), a major constituent of the outer membrane of Gammaproteobacteria, which exhibit superior Toll-like receptor 4 (TLR4) stimulatory capacity compared to the less acylated LPS present from other Gram-negative bacteria (Larsen et al, 2012(Larsen et al, , 2015Li et al, 2013;Brix et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Short-Term Amoxicillin-Induced Perturbation of the Gut Microbiota Promotes Acute Intestinal Immune Regulation in Brown Norway Rats

et al. 2020

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The intestinal gut microbiota is essential for maintaining host health. Concerns have been raised about the possible connection between antibiotic use, causing microbiota disturbances, and the increase in allergic and autoimmune diseases observed during the last decades. To elucidate the putative connection between antibiotic use and immune regulation, we have assessed the effects of the antibiotic amoxicillin on immune regulation, protein uptake, and bacterial community structure in a Brown Norway rat model. Daily intra-gastric administration of amoxicillin resulted in an immediate and dramatic shift in fecal microbiota, characterized by a reduction of within sample (α) diversity, reduced variation between animals (β diversity), increased relative abundance of Bacteroidetes and Gammaproteobacteria, with concurrent reduction of Firmicutes, compared to a water control group. In the small intestine, amoxicillin also affected microbiota composition significantly, but in a different way than observed in feces. The small intestine of control animals was vastly dominated by Lactobacillus, but this genus was much less abundant in the amoxicillin group. Instead, multiple different genera expanded after amoxicillin administration, with high variation between individual animals, thus the small intestinal α and β diversity were higher in the amoxicillin group compared to controls. After 1 week of daily amoxicillin administration, total fecal IgA level, relative abundance of small intestinal regulatory T cells and goblet cell numbers were higher in the amoxicillin group compared to controls. Several bacterial genera, including Escherichia/Shigella, Klebsiella (Gammaproteobacteria), and Bifidobacterium, for which the relative abundance was higher in the small intestine in the amoxicillin group than in controls, were positively correlated with the fraction of small intestinal regulatory T cells. Despite of epidemiologic studies showing an association between early life antibiotic consumption and later prevalence of inflammatory bowel diseases and food allergies, our findings surprisingly indicated that amoxicillin-induced perturbation of the gut microbiota promotes acute immune regulation. We speculate that the observed increase in relative abundance of small intestinal regulatory T cells is partly mediated by immunomodulatory lipopolysaccharides derived from outgrowth of Gammaproteobacteria.

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Section: Discussionmentioning

confidence: 99%

Short-Term Amoxicillin-Induced Perturbation of the Gut Microbiota Promotes Acute Intestinal Immune Regulation in Brown Norway Rats

et al. 2020

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“…NVP-1703 is a probiotic mixture of B. longum IM55 (from the human gut bacteria collection) as a T-cell regulator and L. plantarum IM76 (from the fermented food bacteria collection) as a macrophage NF-κB inhibitor. We previously reported that NPV-1703 regulates innate and adaptive immunity in animal and in vitro studies [ 15 , 16 ]. In the present study, NVP-1703 treatment significantly improved TNSS and RCAT scores by increasing levels of IL-10, which corresponds to the anti-allergic effect of B. longum IM55 and L. plantarum IM76.…”

Section: Discussionmentioning

confidence: 99%

“…In a randomized, open-label crossover study of Lactobacillus johnsonii EM1 in 7–12-year-old children with perennial AR, treatment with EM1 and levocetirizine combined significantly increased serum TGF-β levels compared with levocetirizine alone at week 12 [ 14 ]. We previously reported that the noble probiotic NVP-1703, a mixture of Bifidobacterium longum IM55 and Lactobacillus plantarum IM76, mitigated ovalbumin (OVA)- and house dust mite allergen (HDMA)-induced AR in mice, reducing IL-4, IL-5, IL-13, and IgE levels in nasal mucosa, bronchial alveolar lavage fluid, and blood and increasing the level of IL-10 [ 15 , 16 ], suggesting that probiotics may suppress Th2 response by promoting Treg activity.…”

Section: Introductionmentioning

confidence: 99%

Probiotic NVP-1703 Alleviates Allergic Rhinitis by Inducing IL-10 Expression: A Four-week Clinical Trial

et al. 2020

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Although several recent studies reported that probiotics might be beneficial for allergic rhinitis (AR), the effect of probiotics on AR is not consistent and have not been reproduced between studies. We aimed to determine the efficacy and safety of probiotic NVP-1703, a mixture of Bifidobacterium longum and Lactobacillus plantarum, in subjects with perennial AR. Adult subjects with perennial AR received either NVP-1703 (n = 47) or placebo (n = 48) for four weeks. Total nasal symptom scores (TNSS), rhinitis control assessment test (RCAT), blood eosinophil count, allergen-specific IgE, and immunological parameters in serum and urine were compared at baseline and after four weeks. TNSS changes from baseline at weeks 1, 3, and 4 were significant between the NVP-1703 and placebo groups (p = 0.033, 0.031, and 0.029, respectively). RCAT score showed significant differences between the NVP-1703 and placebo groups (p = 0.049) at week 4. Dermatophagoides farinae-specific IgE levels and serum IL-10 levels were significantly different between the NVP-1703 and placebo groups (p = 0.033 and p = 0.047, respectively). IL-10/IL-4 and IL-10/IL-13 ratios were different between the NVP-1703 and placebo groups at week 4 (p = 0.046 and 0.018, respectively). NVP-1703 treatment reduced urinary prostaglandin F2α and leukotriene E4 levels (p > 0.05). Therefore, NVP-1703 can be treatment option for perennial AR.

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“…It has been reported that allergic symptoms can be alleviated by the intake of certain foods, components thereof, or probiotics such as tea (extracts of Camellia sinensis L.), catechin, lactic acid bacteria, and bifidobacteria (8,9). In particularly, tea catechins, a group of polyphenolic compounds like epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me) and epigallocatechin-3-O-(4-O-methyl) gallate (EGCG4"Me), exert strong anti-allergic effects (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Oral Administration of Flavonifractor plautii Strongly Suppresses Th2 Immune Responses in Mice

et al. 2020

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The bacterium Flavonifractor plautii (FP), which is found in human feces, has been reported to participate in catechin metabolism in the gut, but this bacterium's effects on immune function are unclear. We assessed the effect of oral administration of FP on the immune response in ovalbumin (OVA) -sensitized mice. We demonstrated that the FP treatment suppressed interleukin (IL)-4 in splenocytes and OVA-specific IgE production in serum from OVA-sensitized mice. Moreover, oral administration of FP augmented CD4 + CD25 + T cells and CD103 + CD11c + DCs. In animals of the FP group, the proportion of FP was increased in the mesenteric lymph nodes (MLNs), as was the proportion of Deferribacteres in the cecum. Oral administration of FP may inhibit the Th2 immune response by incorporation into the MLNs and/or by inducing changes in the gut microbiota. Thus, FP may be useful in alleviating antigen-induced Th2 immune responses.

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Bifidobacterium longum IM55 and Lactobacillus plantarum IM76 alleviate allergic rhinitis in mice by restoring Th2/Treg imbalance and gut microbiota disturbance

Cited by 59 publications

References 56 publications

Short-Term Amoxicillin-Induced Perturbation of the Gut Microbiota Promotes Acute Intestinal Immune Regulation in Brown Norway Rats

Short-Term Amoxicillin-Induced Perturbation of the Gut Microbiota Promotes Acute Intestinal Immune Regulation in Brown Norway Rats

Probiotic NVP-1703 Alleviates Allergic Rhinitis by Inducing IL-10 Expression: A Four-week Clinical Trial

Oral Administration of Flavonifractor plautii Strongly Suppresses Th2 Immune Responses in Mice

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