Biflavonoids from Daphne feddei and Their Inhibitory Activities against Nitric Oxide Production

Abstract

Bioassay-directed fractionation led to the isolation of two new biflavonoids and 13 known biflavonoids from a sample of the dried stem barks of Daphne feddei. The structures of compounds 1 and 2 were elucidated as 2؆-methoxy-daphnodorin C (1) and 2؆-methoxy-2-epi-daphnodorin C (2) on the basis of detailed spectroscopic analysis and X-ray crystallography. All 15 biflavonoids were tested for inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compounds 1, 2, 8, 10, 14 and 15 showed varying degrees of inhibitory activities against the production of NO in tested concentration of 25, 50, 75 and 100 m mg/ml.

Flavonoids from Daphne aurantiaca and Their Inhibitory Activities against Nitric Oxide Production

“…5 Twenty five isolates were tested for inhibitory activities against LPS-induced NO production in RAW 264.7 macrophages, expert for compounds 8-13 and 5, which had been reported in the our previous paper about Daphne feddei level. 5) Compounds 21 and 24 showed potent inhibitory activities against the production of NO with IC 50 values of 0.006 and 0.076 mM, respectively.…”

“…1) A literature search revealed no previous phytochemical studies on this plant. In the course of our study on the constituents of thymelaeaceous plants, [2][3][4][5] three new flavonoids, compounds 1-3, together with 29 known flavonoids, were isolated from the titled plant. This paper concerned with the structural elucidation of compounds 1-3 and inhibitory activities of 32 compounds against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophages.…”

Secolignans, Neolignans and Phenylpropanoids from Daphne feddei and Their Biological Activities

“…acetone extract prepared from the leaves and stems of D. feddei was partitioned between EtOAc and H 2 O. The EtOAc layer was subjected repeatedly to column chromatography on Si gel, Sephadex LH-20, RP-18, and preparative HPLC to afford compounds 1-11, including two new secolignans and one new neolignan, named feddeiphenols A-C (1-3), together with eight known compounds, kadangustin J (4), 5) surinamensin (5), 6) virolin (6), 6) rosmarinic acid (7), 7) clinopodic acid A (8), 8) vladinol F (9), 9) 9Ј-dehydroxy-vladinol F (10), 10) and isocubebin (11), 4) The structures of the compounds 1-11 were determined as shown in Fig. 1 ).…”

“…It has been used as a traditional Chinese medicine, named "Dian Rui Xiang" for the treatment of the injuries from falls and bruises. 1) The previous phytochemical studies on Daphne feddei showed that diterpenes, 2) phenylpropanoids, 3) as well as flavonoids 4) are the major components of this plant.…”

A New Tigliane-Type Diterpenoid from Daphne genkwa

“…[18][19][20][21] Taken together, our results indicate that the diterpenoid compounds from the flower buds of D. genkwa might be valuable candidates for the treatment of various inflammatory disease caused by excessive production of NO.…”