In today's turbulent times, businesses must cope with an increasing focus on digital technology, automation and robotics systems. It represents the fourth industrial revolution, called Industry 4.0. The goals of Industry 4.0 are achieving higher levels of operational efficiency and productivity, as well as a higher level of automation. Concerning Industry 4.0, therefore, not only the technological aspect but also the size of the organization, the scope of business by industry and other elements are considered and analyzed based on Mann-Whitney U test and Fisher test. According to statistical analysis, it was found that the size of the company affects its relationship to Industry 4.0, and we can say that the larger the business, the more it is affected by Industry 4.0. This influence was manifested primarily in companies operating in the field of commercial activities, or Agriculture and Construction. In terms of the relationship between the written strategy and Industry 4.0, it can be stated that the link between them has occurred in small and medium-sized enterprises operating in the areas of Professional and Administrative Activities, Manufacturing and Wholesale, Transport and Accommodation. In contrast, this effect has not been proven in micro-enterprises. Implications for a Central European audience: The paper primarily deals with the analysis of the relationship between enterprise size and Industry 4.0 and the study of the relationship between the division of enterprises by industry in relation to CZ-NACE. A significant difference was found between the enterprises affected and unaffected by Industry 4.0 when evaluating the size of the enterprise. Enterprises involved by Industry 4.0 employ more workers, which may be due to the introduction of automation and robotic automation, which means the initial phase. As the results further show, the written strategy is mostly the domain of medium-sized enterprises, with only 29 % of micro-enterprises and 41 % of small enterprises having it defined.