Bilan d’une azoospermie et évaluation histologique de la spermatogenèse

Robin, Geoffroy; Boitrelle, Florence; Leroy, Xavier; Peers, Marie-Claire; Marcelli, F.; Rigot, Jean-Marc; Mitchell, Valérie

doi:10.1016/j.annpat.2010.03.015

Cited by 27 publications

(13 citation statements)

References 70 publications

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“…Hypospermatogenesis is prevalent in azoospermic patients and is associated with male infertility. It is characterized by a lower production of spermatozoids in seminiferous tubules (18,19). It is well known that abnormal apoptosis of spermatogenic cells is implicated in hypospermatogenesis (20–23).…”

Section: Discussionmentioning

confidence: 99%

PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis

Zhao

Zhang

2019

Mol Med Report

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Phosphoglycerate mutase 1 (PGAM1) is reported to be involved in spermatogenic dysfunction. However, the association between PGAM1 and busulfan-induced hypospermatogenesis and spermatogenic cell apoptosis remains unclear. The aim of the current study was to investigate the association between PGAM1 expression and busulfan-induced hypospermatogenesis, and the effect of PGAM1 expression on spermatogenic cell apoptosis. PGAM1 expression was detected in mouse models of busulfan-induced hypospermatogenesis by western blotting, reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. Then, spermatogenic cell apoptosis in mouse models of busulfan-induced hypospermatogenesis was assessed by TUNEL assay. The effect and potential mechanism of PGAM1 downregulation on spermatogenic cells were further investigated. The results indicated that PGAM1 expression was significantly downregulated in the mouse models of busulfan-induced hypospermatogenesis, compared with those with normal spermatogenesis (P<0.05). Furthermore, the TUNEL assay revealed that the apoptosis of spermatogenic cells was accelerated in the mouse model of busulfan-induced hypospermatogenesis. In addition, PGAM1 knockdown promoted the apoptosis of spermatogenic cells in vitro, which was associated with the P53/Caspase 3/Caspase 6/Caspase 9 signaling pathway. In conclusion, these data indicate that PGAM1 knockdown is associated with busulfan-induced hypospermatogenesis and contributes to spermatogenic cell apoptosis by regulating the P53/Caspase 3/Caspase 6/Caspase 9 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis

Zhao

Zhang

2019

Mol Med Report

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“…These data are similar to those reported in the existing literature (45%–63%; 2–5, 11–13). In general, when cTESE is used, the success rates of sperm retrieval in NOA patients are reported to be33.7%–50.9% [ 11 , 12 , 13 , 14 , 15 , 16 ], however, lower rates are reported to be 16%–33% [ 17 ]. SRR from cTESE is lower than that for micro-TESE, which commonly shows rates of50.8%–56.0% [ 11 , 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…In a review of the literature, Esteves et al revealed that TESA retrieval rates from NOA patients ranged from 10%–30%, except in cases of previously successful TESA or testicular histopathology showing hypospermatogenesis. In such cases, TESA SRR ranges from 70%–100% [ 17 ]. Another systematic review showed that the mean SRR for TESE was 49.5%, while another paper reported that the SRR for TESA and TESE was 41.8% and 50.9%, respectively ( p = 0.04) [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

A clinical trial on the consistency of bilateral testicular tissue histopathology and Johnsen score: single side or bilateral side biopsy?

et al. 2018

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To evaluate and compare left and right testicular tissue histopathology and Johnsen score, and to investigate the necessity for bilateral testicular biopsy. We recruited180 patients with non-obstructiveazoospermia (NOA) on testicular biopsy who had undergonetesticular sperm aspiration (TESA). Pathological sections of testicular tissue were diagnosed by specially-assigned doctors, who evaluated pathological findings, determined the Johnsen score and confirmed for the presence or absence of sperm. Sperm positive rates for left and right testicular histopathology were 55.0% and 51.7% respectively, and the proportion of Johnsen scores≥8 for left and right testes were 53.3% and 50.0%, respectively. Cohen kappa values revealed that the identification of sperm in bilateral testicular samples was not consistent and was related to random effects; Optimized cut-off value for bilateral testicular volume was 11ml (Johnsen score ≥8), and optimized cut-off values of E2 on left and right testes were 144.5pmol/L and 133.5 pmol/L (Johnsen score≤7). However, age, serum prolactin (PRL), follicle stimulating hormone (FSH), luteinizing hormone (LH) and total testosterone (TT) levels were not accurate predictors for the existence of testicular sperm. There was nostatistical significance between left and right testicular histopathology in terms of sperm positive rates or Johnsen score; the Johnsen score were caused entirely by random effects and a score from one side could not represent the other side. Therefore, we recommend that both testes need to undergo surgery when NOA patients undergo testicular biopsy or sperm retrieval.

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“…Such patients are about 25 to 50% of total andrological cases [ 80 , 81 ]. This type of aspermatogenic patient can be divided in three groups based on histopathologic findings in biopsies, including a) complete absence of germ cell [ 134 ] b) mitotic arrest during spermatogenesis [ 135 ] and c) pre-meiotic arrest [ 136 ]. Spermatogenic arrest is one of the major causes of male factor infertility.…”

Section: Infertility Treatmentmentioning

confidence: 99%

Progress and future prospect of in vitro spermatogenesis

Ibtisham

Xiao

et al. 2017

Oncotarget

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Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40–50% is due to “male factor” infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment. Developments in reproductive biotechnology have made it possible to rescue fertility and uphold biological fatherhood. In vitro production of haploid male germ cell is a powerful tool, not only for the treatment of infertility including oligozoospermic or azoospermic patient, but also for the fertility preservation in pre-pubertal boys whose gonadal function is threatened by gonadotoxic therapies. Genomic editing of in-vitro cultured germ cells could also potentially cure flaws in spermatogenesis due to genomic mutation. Furthermore, this ex-vivo maturation technique with genomic editing may be used to prevent paternal transmission of genomic diseases. Here, we summarize the historical progress of in vitro spermatogenesis research by using organ and cell culture techniques and the future clinical application of in vitro spermatogenesis.

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Bilan d’une azoospermie et évaluation histologique de la spermatogenèse

Cited by 27 publications

References 70 publications

PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis

PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis

A clinical trial on the consistency of bilateral testicular tissue histopathology and Johnsen score: single side or bilateral side biopsy?

Progress and future prospect of in vitro spermatogenesis

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