Bilayer undulation dynamics in unilamellar phospholipid vesicles: Effect of temperature, cholesterol and trehalose

Brüning, Beate-Annette; Prévost, Sylvain; Stehle, Ralf; Steitz, Roland; Falus, Péter; Faragó, B.; Hellweg, Thomas

doi:10.1016/j.bbamem.2014.06.006

Cited by 29 publications

(27 citation statements)

References 81 publications

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“…In contrast, neutron spin echo (NSE), as the x-ray method, measures at smaller length scales than the classical methods. NSE values of K C /kT for DMPC range from 15 [24] to about 20 [25][26][27]. These agree better with the no tilt values in Fig.…”

supporting

confidence: 86%

X-ray scattering reveals molecular tilt is an order parameter for the main phase transition in a model biomembrane

Nagle

2017

Phys. Rev. E

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Synchrotron diffuse x-ray scattering data reveal a dramatic softening of the molecular tilt modulus K_{θ} of the model biomembrane composed of DMPC lipids as the temperature is lowered towards the main phase transition temperature at T_{M}=24^{∘}C. Spontaneous tilt occurs below T_{M}, suggesting that tilt is a symmetry breaking order parameter. Consistent with this hypothesis, it is also found that a different lipid POPS has no spontaneous tilt below its T_{M} at 14^{∘}C and correspondingly its tilt modulus did not soften as T_{M} was approached from above. As previously known, the bending modulus K_{C} of DMPC also softens close to T_{M}, but unlike the tilt modulus, K_{C} has a maximum 3^{∘} above T_{M}, which also marks the limit of the well-known anomalous swelling regime. Tilt adds a different perspective to our previous understanding of the main phase transition in lipid bilayers.

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supporting

confidence: 86%

X-ray scattering reveals molecular tilt is an order parameter for the main phase transition in a model biomembrane

Nagle

2017

Phys. Rev. E

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“…The three main experiments to determine bending rigidities for pure lipid bilayers are diffuse x-ray scattering of flat bilayers [43–49], force-based methods to deform a membrane such as the pipette aspiration technique and pulling membrane nanotubes [50–58,137–144], and fluctuating shape analysis of GUVs [51,59,60,145–156]. To measure the rigidity of SUVs and purified synaptic vesicles [64], neutron spin echo (NSE) [61–63], and atomic force-microscopy (AFM) experiments have been used [65–69]. There are no experimental reports on the influence of adsorbed αSyn on bending rigidity.…”

Section: Protein-induced Alterations In Membrane Materials Propertiesmentioning

confidence: 99%

“…In the case of flat membranes, we discuss the use of diffuse x-ray scattering [43–49], pipette aspiration and tether pulling [50–58], and fluctuation analysis [50,51,59,60]. In the more challenging case of small vesicles, we explore neutron spin echo (NSE) [61–63], and atomic force microscopy (AFM) experiments [64–69]. Finally, we describe the most recent efforts, including our own, to accurately capture bending rigidity from simulations of fluctuating membranes, both in flat bilayers and small vesicles [70–74].…”

Section: Introductionmentioning

confidence: 99%

Membrane remodeling and mechanics: Experiments and simulations of α-Synuclein

West

Brummel

Braun

et al. 2016

Biochimica et Biophysica Acta (BBA) - Biomembranes

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We review experimental and simulation approaches that have been used to determine curvature generation and remodeling of lipid bilayers by membrane-bending proteins. Particular emphasis is placed on the complementary approaches used to study α-Synuclein (αSyn), a major protein involved in Parkinson's disease (PD). Recent cellular and biophysical experiments have shown that the protein 1) deforms the native structure of mitochondrial and model membranes; and 2) inhibits vesicular fusion. Today's advanced experimental and computational technology has made it possible to quantify these protein-induced changes in membrane shape and material properties. Collectively, experiments, theory and multi-scale simulation techniques have established the key physical determinants of membrane remodeling and rigidity: protein binding energy, protein partition depth, protein density, and membrane tension. Despite the exciting and significant progress made in recent years in these areas, challenges remain in connecting biophysical insights to the cellular processes that lead to disease. This article is part of a Special Issue entitled: Membrane Proteins edited by J.C. Gumbart and Sergei Noskov.

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“…[1][2][3][4][5] These properties have stabilizing effects on biological membranes under extreme thermal and mechanical conditions, which are particularly important during the process of dehydration. [1][2][3][4][5] These properties have stabilizing effects on biological membranes under extreme thermal and mechanical conditions, which are particularly important during the process of dehydration.…”

Section: Introductionmentioning

confidence: 99%

Molecular dynamics simulations and NMR spectroscopy studies of trehalose–lipid bilayer systems

Kapla

Engström

Stevensson

et al. 2015

Phys. Chem. Chem. Phys.

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The disaccharide trehalose (TRH) strongly affects the physical properties of lipid bilayers. We investigate interactions between lipid membranes formed by 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and TRH using NMR spectroscopy and molecular dynamics (MD) computer simulations. We compare dipolar couplings derived from DMPC/TRH trajectories with those determined (i) experimentally in TRH using conventional high-resolution NMR in a weakly ordered solvent (bicelles), and (ii) by solid-state NMR in multilamellar vesicles (MLV) formed by DMPC. Analysis of the experimental and MD-derived couplings in DMPC indicated that the force field used in the simulations reasonably well describes the experimental results with the exception for the glycerol fragment that exhibits significant deviations. The signs of dipolar couplings, not available from the experiments on highly ordered systems, were determined from the trajectory analysis. The crucial step in the analysis of residual dipolar couplings (RDCs) in TRH determined in a bicelle-environment was access to the conformational distributions derived from the MD trajectory. Furthermore, the conformational behavior of TRH, investigated by J-couplings, in the ordered and isotropic phases is essentially identical, indicating that the general assumptions in the analyses of RDCs are well founded.

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Bilayer undulation dynamics in unilamellar phospholipid vesicles: Effect of temperature, cholesterol and trehalose

Cited by 29 publications

References 81 publications

X-ray scattering reveals molecular tilt is an order parameter for the main phase transition in a model biomembrane

X-ray scattering reveals molecular tilt is an order parameter for the main phase transition in a model biomembrane

Membrane remodeling and mechanics: Experiments and simulations of α-Synuclein

Molecular dynamics simulations and NMR spectroscopy studies of trehalose–lipid bilayer systems

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