Bile salt surfactants in micellar electrokinetic capillary chromatography

Cole, Roderic O.; Sepaniak, Michael J.; Hinze, Willie L.; Gorse, J.; Oldiges, Kimberly

doi:10.1016/s0021-9673(01)87126-0

Cited by 178 publications

(44 citation statements)

References 26 publications

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“…One method available to enhance the separation of complex samples is the use of organic modifiers. The migration time, migration order, and selectivity can all be affected by acetonitrile or methanol [23][24][25]. In addition, organic modifiers may change the polarity, conductivity, and viscosity of the BGE as well as the zeta potential.…”

Section: Optimization Of the Separationmentioning

confidence: 99%

Detection of a urinary biomaker for oxidative DNA damage 8-hydroxydeoxyguanosine by capillary electrophoresis with electrochemical detection

Weiss

Lunte

2000

Electrophoresis

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Abstract8-Hydroxydeoxyguanosine (8-OHdG) is present in urine as a result of oxidative DNA damage associated with age-related diseases such as cancer. In this report a method is presented for the detection of 8-OHdG in human morning urine utilizing capillary electrophoresis with electrochemical detection (CEEC). The limit of detection for a aqueous standard of 8-OHdG is 50 nM (signal to noise ratio S/N = 3). A single solid-phase extraction (SPE) step with a C18 column is used for sample cleanup and 20-fold preconcentration of the urine before analysis by CEEC. Optimized conditions for analysis of extracted urine are E app = 0.5 V vs. Ag/AgCl with 20 mM sodium borate/20% MeOH v/v, pH 9, as the background electrolyte, and a separation voltage of 22 kV. The concentration of 8-OHdG varied from 6 to 86 nM with an average value of 42 ± 26.9 nM for four healthy female and four healthy male subjects between the ages of 23 and 43.

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Section: Optimization Of the Separationmentioning

confidence: 99%

Detection of a urinary biomaker for oxidative DNA damage 8-hydroxydeoxyguanosine by capillary electrophoresis with electrochemical detection

Weiss

Lunte

2000

Electrophoresis

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“…Warner and coworkers [10] have successfully separated 16 PAHs [9] and 12 monomethyl-benz[a]anthracene isomers by exploring a high-purity T-type polymerized surfactant with an undecenyl (C 11 ) and a sulfate group. Moreover, several other additives were reported, and they included a bile salt surfactant [11], a high-concentration ionic surfactant [12], and a neutral and anionic CD mixture [13]. Among these techniques, CD-modified MEKC (CD-MEKC) has been demonstrated to be an efficient separation method for PAHs [14].…”

Section: Introductionmentioning

confidence: 99%

Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates

Song

Peng

Lin

2006

J of Separation Science

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Original PaperComparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates Naphthalene, fluorene, pyrene, anthracene, phenanthrene, and chrysene were successfully separated by CD-modified MEKC (CD-MEKC) using 20 mM borate (pH 9.0) containing 90 mM SDS and 75 mM b-CD. Two online stacking methods, i. e., sweeping and field-enhanced sample injection (FESI), were explored to enhance the detection sensitivity. The influences of some crucial parameters in sweeping and FESI procedures were investigated. For FESI method, a plug of water and low-conductivity sample matrix was used to increase the stacking efficiency. Compared with the sweeping method, FESI can increase the sensitivity in the range of 10 -20-fold. The proposed method was used for the analysis of polycyclic aromatic hydrocarbons in airborne particulates.

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“…Nevertheless, it is generally agreed that the choice of the surfactant is the most important consideration for optimizing selectivity [29,30]. Although SDS is certainly the most popular anionic surfactant, other surfactants such as bile salts have proved to be very successful to separate nonpolar analytes that could not be resolved using SDS [31,32]. Mixing surfactants with different structural and polar properties to generate mixed micelles has the potential to provide variable micellar phases.…”

Section: Introductionmentioning

confidence: 99%

Mixed micellar electrokinetic capillary chromatography separation of depolymerized grape procyanidins

et al. 2003

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Oligomeric procyanidins are potent antioxidant polyphenols of potential interest as disease-preventing agents. Their efficiency depends on the size and composition of their oligomeric structures. The mean degree of polymerization of these compounds is usually estimated by thiolysis with thiol-alpha-toluene followed by analysis using high-performance liquid chromatography (HPLC). We show the development of a mixed micellar electrokinetic chromatography (MEKC) method for the separation of the major components obtained after thiolysis with cysteamine (catechins and their cysteamine conjugates). MEKC studies using sodium dodecyl sulfate (SDS as pseudostationary phase led to long migration times, e.g., with 100 mM SDS, at pH 7, the solutes were separated in about 40 min), while the use of sodium cholate (SC) produced an elution window relatively short. Using a mixed micellar SC-SDS system (50 mM phosphate at pH 7 containing 40 mM SC and 10 mM SDS), it is possible to separate these compounds in less than 15 min. The proposed method is useful to separate the major components of the thiolysate in effluents from food processing (e.g., skins and seeds from grape and apple) considered as potential procyanidin sources.

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Bile salt surfactants in micellar electrokinetic capillary chromatography

Cited by 178 publications

References 26 publications

Detection of a urinary biomaker for oxidative DNA damage 8-hydroxydeoxyguanosine by capillary electrophoresis with electrochemical detection

Detection of a urinary biomaker for oxidative DNA damage 8-hydroxydeoxyguanosine by capillary electrophoresis with electrochemical detection

Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates

Mixed micellar electrokinetic capillary chromatography separation of depolymerized grape procyanidins

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