Billings reservoir water used for human consumption presents microbiological contaminants and induces both behavior impairments and astrogliosis in zebrafish

Leme, E.; Silva, Ericka P.; Rodrigues, P.; Silva, Igor R.; Martins, M.F.; Bondan, Eduardo Fernandes; Bernardi, Maria Martha; Kirsten, Thiago Berti

doi:10.1016/j.ecoenv.2018.06.009

Cited by 12 publications

(18 citation statements)

References 42 publications

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“…Other advantages of the zebrafish model include rapid embryonic development, high fecundity, quite low housing and experimental costs [ 39 , 41 ]. Moreover, zebrafish possess a vertebrate neural structural organization and have already been established as a valuable model system to study alteration of astrocyte homeostasis in vivo [ 42 , 43 , 44 ], including astrogliosis [ 45 , 46 ]. It was found that zebrafish have two intracellular cobalamin-dependent enzymes, which share an overall amino acid identity with the corresponding human enzymes of approx.…”

Section: Discussionmentioning

confidence: 99%

Astrogliosis in an Experimental Model of Hypovitaminosis B12: A Cellular Basis of Neurological Disorders due to Cobalamin Deficiency

Rzepka

Rok

Kowalska

et al. 2020

Cells

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Cobalamin deficiency affects human physiology with sequelae ranging from mild fatigue to severe neuropsychiatric abnormalities. The cellular and molecular aspects of the nervous system disorders associated with hypovitaminosis B12 remain largely unknown. Growing evidence indicates that astrogliosis is an underlying component of a wide range of neuropathologies. Previously, we developed an in vitro model of cobalamin deficiency in normal human astrocytes (NHA) by culturing the cells with c-lactam of hydroxycobalamin (c-lactam OH-Cbl). We revealed a non-apoptotic activation of caspases (3/7, 8, 9) in cobalamin-deficient NHA, which may suggest astrogliosis. The aim of the current study was to experimentally verify this hypothesis. We indicated an increase in the cellular expression of two astrogliosis markers: glial fibrillary acidic protein and vimentin in cobalamin-deficient NHA using Western blot analysis and immunocytochemistry with confocal laser scanning microscopy. In the next step of the study, we revealed c-lactam OH-Cbl as a potential non-toxic vitamin B12 antagonist in an in vivo model using zebrafish embryos. We believe that the presented results will contribute to a better understanding of the cellular mechanism underlying neurologic pathology due to cobalamin deficiency and will serve as a foundation for further studies.

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Section: Discussionmentioning

confidence: 99%

Astrogliosis in an Experimental Model of Hypovitaminosis B12: A Cellular Basis of Neurological Disorders due to Cobalamin Deficiency

Rzepka

Rok

Kowalska

et al. 2020

Cells

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“…The water from three areas of the Billings reservoir intended for human use, as the RP, RG, and BO rivers was previously studied by Leme et al (2018). RP has been considered an area for water resource (Brasil, 2015).…”

Section: /9mentioning

confidence: 99%

Billings Reservoir (Brazil): Chemical Studies on Water and Chemical and Morphological Studies on Zebrafish Gills

et al. 2021

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Previous study of this research group revealed that water from the Billings reservoir (Brazil) intended for human use (water supply and seafood) has microbiological contamination and causes lethality and brain and behavioral impairments in zebrafish. The objective of this study was to understand what have induced these impairments in the animal model. Chemical analyses on water samples from Rio Pequeno (RP), Rio Grande (RG), and Bororé (BO) rivers, as well as chemical and morphological analyses on zebrafish gills exposed to those waters were performed. Waters samples from RP, RG, and BO presented high levels of phosphorus. BO water and fish gills exposed to this water presented high levels of nitrogen. RG water caused potassium contamination in gills. Phosphorus, nitrogen, and potassium are indicators of anthropogenic pollution. RG water and fish gills exposed to this water presented low levels of calcium, which might be an indication of chemical imbalance that could lead to health problems in aquatic animals. RG and BO waters resulted in zirconium contamination in gills. BO water induced respiratory lamellae thickening in the gills, which may be the underlying mechanism for the observed hypoxia. In conclusion, behavioral, brain, and respiratory defects observed previously were induced by chemical and morphologicalb disturbances due to anthropogenic pollution in the Billings reservoir.

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“…The reservoir has multiple-use, including hydropower generation, water supply for 4.5 million people, and industries, irrigation, shery, and ood control. The basin has a narrow central body which measures over 20 km and to which eight branches, namely Rio Grande, Rio Pequeno, Capivari, Pedra Branca, Taquacetuba, Bororé, Cocaia, and Alvarenga rivers, converge [3][4][5]. The reservoir has undergone considerable changes in its properties since the 1940s.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Bacterial Communities from the Surface and Adjacent Bottom Layers Water of Billings Reservoir

Marcondes

Nascimento

Pessôa

et al. 2021

Preprint

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Here, we describe the microbial diversity and physicochemical properties in freshwater samples from the surface and bottom layer of Billings reservoir in São Paulo state, Brazil. Twenty-two matched samples were characterized using the 16S rRNA gene Illumina MiSeq platform. Taxonomical composition revealed an abundance of Cyanobacteria phyla, followed by Proteobacteria, with 1903 and 2689 known bacterial genera in the surface and deep-water layers, respectively. Shannon diversity index ranging from 2.3 - 5.39 and 4.04 - 6.86 in the surface and bottom layer, respectively. Among the 120 pathogenic genera identified, Flavobacterium was the most predominant genus. Temperature and phosphorus concentration were the most influential factors in shaping the microbial communities of both layers. Predictive functional analysis suggests that the reservoir is enriched in motility genes involved in the flagellar assembly. The overall results present new information on the significantly altered diversity composition of the bacterial community detected in Billings freshwater reservoir.

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Billings reservoir water used for human consumption presents microbiological contaminants and induces both behavior impairments and astrogliosis in zebrafish

Cited by 12 publications

References 42 publications

Astrogliosis in an Experimental Model of Hypovitaminosis B12: A Cellular Basis of Neurological Disorders due to Cobalamin Deficiency

Astrogliosis in an Experimental Model of Hypovitaminosis B12: A Cellular Basis of Neurological Disorders due to Cobalamin Deficiency

Billings Reservoir (Brazil): Chemical Studies on Water and Chemical and Morphological Studies on Zebrafish Gills

Comparison of Bacterial Communities from the Surface and Adjacent Bottom Layers Water of Billings Reservoir

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