Binary (Split) Light‐up Aptameric Sensors

Kolpashchikov, Dmitry M.; Spelkov, Alexander A.

doi:10.1002/anie.201914919

Cited by 49 publications

(21 citation statements)

References 103 publications

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“…Compared to the known LODs for RNA-targeted biosensors, the sensitivity of SDFS was at least not lower to Spinach aptamer (LOD is 1.9-5.3 nM in similar conditions) and a little bit less than Dapoxyl aptamer (LOD is 0.44 nM). At the same time, none of the two aptamers mentioned above was tested on full-length mRNA [26].…”

Section: Resultsmentioning

confidence: 99%

A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA

Bryushkova

Gandalipov

Nuzhina

2020

Beilstein J. Org. Chem.

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Nowadays a variety of biosensors are widely used in different fields, including biomedical diagnostics and self-testing. Nucleic acid-based biosensors are typically applied to detect another nucleic acid, proteins, ions, and several other types of compounds. It is most promising to develop simple and effective biosensors for the use in situations where traditional methods are not available due to their complexity and laboriousness. In this project, a novel smart deoxyribozyme-based fluorescent sensor for the detection of androgen receptor mRNA was developed. It consists of several functional modules including two deoxyribozymes 10–23, an RNA-dependent split malachite green aptamer, and an oligonucleotide platform. Deoxyribozymes specifically release a 27-nucleotide RNA fragment that is readily available for the interaction with the aptamer module. This solves a problem of secondary structure in hybridization with the target sequence of full-length mRNA. It was shown that within 24 hours the proposed sensor specifically recognized both a synthetic 60-nucleotide RNA fragment (LOD is 1.4 nM of RNA fragment at 37 °C) and a full-sized mRNA molecule of the androgen receptor. The constructed sensor is easy to use, has high efficiency and selectivity for the RNA target, and can be reconstructed for the detection of various nucleic acid sequences due to its modular structure. Thus, similar biosensors may be useful for the differential diagnosis.

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Section: Resultsmentioning

confidence: 99%

A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA

Bryushkova

Gandalipov

Nuzhina

2020

Beilstein J. Org. Chem.

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“…Upon binding to the targets, two DNA or RNA light-up oligonucleotides can be fused to form fluorogenic dye-binding sites which are stabilized by fluorophore binding, resulting in a fluorescent signal. This molecular sensing method based on light-up aptamers is reversible and allows for signal reduction when the concentration of the analyte is reduced . So far, a number of RNA aptamers for fluorophores including spinach, broccoli, mango, and corn have been isolated and investigated .…”

Section: Antibodies Vs Aptamersmentioning

confidence: 99%

Recent Developments in Aptasensors for Diagnostic Applications

Liu

Wang

et al. 2020

ACS Appl. Mater. Interfaces

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Aptamers are exciting smart molecular probes for specific recognition of disease biomarkers. A number of strategies have been developed to convert target−aptamer binding into physically detectable signals. Since the aptamer sequence was first discovered, a large variety of aptamer-based biosensors have been developed, with considerable attention paid to their potential applications in clinical diagnostics. So far, a variety of techniques in combination with a wide range of functional nanomaterials have been used for the design of aptasensors to further improve the sensitivity and detection limit of target determination. In this paper, the advantages of aptamers over traditional antibodies as the molecular recognition components in biosensors for high-throughput screening target molecules are highlighted. Aptamer−target pairing configurations are predominantly single-or dual-site binding; the design of recognition modes of each aptamer−target pairing configuration is described. Furthermore, signal transduction strategies including optical, electrical, mechanical, and masssensitive modes are clearly explained together with examples. Finally, we summarize the recent progress in the development of aptamer-based biosensors for clinical diagnosis, including detection of cancer and disease biomarkers and in vivo molecular imaging. We then conclude with a discussion on the advanced development and challenges of aptasensors.

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“…The inverse relationship between affinity and selectivity central to hybridization-based technologies is circumvented by the splitting paradigm, as the affinity is imparted by cooperation between shorter fragments that retain selectivity as each individual piece is sensitive to mismatches. Split aptamer sensors have been produced for a variety of targets with a recent review highlighting some of the detection schemes and designs [ 23 , 24 ]. While many single target interrogating aptamer sensors have been developed, the literature is much less developed for multiplex aptamer-based assays.…”

Section: Introductionmentioning

confidence: 99%

Binary (Split) Light‐up Aptameric Sensors

Cited by 49 publications

References 103 publications

A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA

A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA

Recent Developments in Aptasensors for Diagnostic Applications

A self-assembling split aptamer multiplex assay for SARS-COVID19 and miniaturization of a malachite green DNA-based aptamer

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