Binding interactions of bacterial lipopolysaccharides to polymyxin B in an amphiphilic carrier ‘sodium deoxycholate sulfate’

Madhumanchi, Sreenu; Suedee, Roongnapa; Nakpheng, Titpawan; Tinpun, Kittiya; Temboot, Pornvichai; Srichana, Teerapol

doi:10.1016/j.colsurfb.2019.110374

Cited by 21 publications

(8 citation statements)

References 35 publications

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“…Since the cationic cyclic peptide in the structure of polymyxin B can bind to the sulfate ion in the active center of endotoxin, resulting in the inactivation of endotoxin, which has the effect of antagonism against endotoxin. So polymyxin B has important value in the clinical treatment of endotoxin-induced sepsis [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy and safety of polymyxin B in carbapenem-resistant gram-negative organisms infections

Xia

Jiang

2021

BMC Infect Dis

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Objective To investigate how to use polymyxin B rationally in order to produce the best efficacy and safety in patients with carbapenem-resistant gram-negative organisms (CRO) infection. Methods The clinical characteristics and microbiological results of 181 patients caused by CRO infection treated with polymyxin B in the First Affiliated Hospital from July 2018 to May 2020 were retrospectively analyzed. The bacterial clearance rate, clinical efficacy, adverse drug reactions and 28 days mortality were evaluated. Results The overall effective rate of 181 patients was 49.72%, the total bacterial clearance rate was 42.0%, and the 28 day all-cause mortality rate was 59.1%. The effective rate and bacterial clearance rate in the group of less than 24 h from the isolation of CRO to the use of polymyxin B were significantly higher than those in the group of more than 24 h. Logistics multivariate regression analysis showed that the predictive factors for effective treatment of CRO with polymyxin B were APACHEII score, duration of polymyxin B treatment, combination of polymyxin B and other antibiotics, and bacterial clearance. 17 cases (9.36%) of acute kidney injury were considered as polymyxin B nephrotoxicity and 4 cases (23.5%) recovered after polymyxin B withdrawal. After 14 days of polymyxin B use, 3 cases of polymyxin B resistance appeared, and there were 2 cases of polymyxin B resistance in the daily dose 1.5 mg/kg/day group. Conclusion For CRO infection, the treatment of polymyxin B should be early, combined, optimal dose and duration of treatment, which can achieve better clinical efficacy and microbial reactions, and reduce the adverse reactions and drug resistance.

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Section: Discussionmentioning

confidence: 99%

Efficacy and safety of polymyxin B in carbapenem-resistant gram-negative organisms infections

Xia

Jiang

2021

BMC Infect Dis

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“…The major peaks of CTAB present in CuNCs/CuF are at 719 cm –1 (rocking mode of the methylene chain), 960 cm –1 (C–N + stretching), 3016 cm –1 (asymmetric stretching band of N + (CH 3 ) 3 groups), 1482 cm –1 (asymmetric scissoring vibrations of CH 3 –N + ), 2917, and 2850 cm –1 (CH 2 asymmetric and symmetric stretching vibrations) . The FTIR spectrum of PmB shows the vibrations corresponding to NH 2 and NH stretching (around 3250 cm –1 ) and amide stretchings at 1657 and 1544 cm –1 . On immobilization of PmB on CuNCs/CuF, the amide bands shifted to 1653 and 1534 cm –1 .…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Approach for the Synthesis of Ultrasmall Cu₁₃ Clusters and Their Application in the Detection of Endotoxin

et al. 2023

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Ultrasmall metal nanoclusters are attractive for their size-dependent optical and electrochemical properties. Here, blue-emitting copper clusters stabilized with cetyltrimethylammonium bromide (CTAB) are synthesized using an electrochemical approach. The electrospray ionization (ESI) analysis reveals that the cluster has 13 copper atoms in the core. The clusters are then used for the electrochemical detection of endotoxina bacterial toxin in Gram-negative bacteria. Differential pulse voltammetry (DPV) is used to detect endotoxins with high selectivity and sensitivity. It shows a lower detection limit of 100 ag mL–1 with a linear range of 100 ag mL–1 to 10 ng mL–1. The sensor is efficient for detecting endotoxins from human blood serum samples.

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“…The few AMP-loaded micelles described in the literature are summarized in Table 1. Temboot et al and Madhumanchi et al prepared polymyxin B-loaded micelles composed of lipid sodium deoxycholate sulfate (SDCS) and showed they maintained antimicrobial activity against planktonic Acinetobacter baumannii and P. aeruginosa and biofilms of the latter, while reducing cytotoxicity to two types of epithelial cells and reducing the hemolytic activity of polymyxin B [82,83]. Moreover, Kumar et al encapsulated the aurein-derived AMP peptide 73c in PEGylated phospholipid micelles and showed that peptide-73c-loaded micelles lost in vitro antimicrobial activity against S. aureus biofilm, while cytotoxicity was reduced against red blood cells and human peripheral blood mononuclear cells compared to a peptide 73c solution.…”

Section: Micellesmentioning

confidence: 99%

Current Advances in Lipid and Polymeric Antimicrobial Peptide Delivery Systems and Coatings for the Prevention and Treatment of Bacterial Infections

et al. 2021

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Bacterial infections constitute a threat to public health as antibiotics are becoming less effective due to the emergence of antimicrobial resistant strains and biofilm and persister formation. Antimicrobial peptides (AMPs) are considered excellent alternatives to antibiotics; however, they suffer from limitations related to their peptidic nature and possible toxicity. The present review critically evaluates the chemical characteristics and antibacterial effects of lipid and polymeric AMP delivery systems and coatings that offer the promise of enhancing the efficacy of AMPs, reducing their limitations and prolonging their half-life. Unfortunately, the antibacterial activities of these systems and coatings have mainly been evaluated in vitro against planktonic bacteria in less biologically relevant conditions, with only some studies focusing on the antibiofilm activities of the formulated AMPs and on the antibacterial effects in animal models. Further improvements of lipid and polymeric AMP delivery systems and coatings may involve the functionalization of these systems to better target the infections and an analysis of the antibacterial activities in biologically relevant environments. Based on the available data we proposed which polymeric AMP delivery system or coatings could be profitable for the treatment of the different hard-to-treat infections, such as bloodstream infections and catheter- or implant-related infections.

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Binding interactions of bacterial lipopolysaccharides to polymyxin B in an amphiphilic carrier ‘sodium deoxycholate sulfate’

Cited by 21 publications

References 35 publications

Efficacy and safety of polymyxin B in carbapenem-resistant gram-negative organisms infections

Efficacy and safety of polymyxin B in carbapenem-resistant gram-negative organisms infections

Electrochemical Approach for the Synthesis of Ultrasmall Cu₁₃ Clusters and Their Application in the Detection of Endotoxin

Current Advances in Lipid and Polymeric Antimicrobial Peptide Delivery Systems and Coatings for the Prevention and Treatment of Bacterial Infections

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Binding interactions of bacterial lipopolysaccharides to polymyxin B in an amphiphilic carrier ‘sodium deoxycholate sulfate’

Cited by 21 publications

References 35 publications

Efficacy and safety of polymyxin B in carbapenem-resistant gram-negative organisms infections

Efficacy and safety of polymyxin B in carbapenem-resistant gram-negative organisms infections

Electrochemical Approach for the Synthesis of Ultrasmall Cu13 Clusters and Their Application in the Detection of Endotoxin

Current Advances in Lipid and Polymeric Antimicrobial Peptide Delivery Systems and Coatings for the Prevention and Treatment of Bacterial Infections

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Electrochemical Approach for the Synthesis of Ultrasmall Cu₁₃ Clusters and Their Application in the Detection of Endotoxin