Binding of adenine nucleotides and pyrophosphate by the purified coupling factor of photophosphorylation

Tiefert, Marjorie A.; Roy, Harry; Moudrianakis, Evangelos N.

doi:10.1021/bi00630a014

Cited by 23 publications

(12 citation statements)

References 22 publications

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“…It is possible that the elution-centrifugation technique that was used to separate the bound and free [32P] P, is inappropriate, especially if [32P]P, dissociates very rapidly from its binding site. This also holds for the experiments reported by Tiefert et al (1977) in which Pj binding was analyzed by exclusion chromatography.…”

Section: Resultssupporting

confidence: 74%

“…After 25 min in the dark followed by 30 min of photoirradiation, the ATPase activity, the bound radioactivity, and the protein content were determined as described under Methods. (•) 50 mM Tris-50 mM Mes-1 mM MgS04, pH 7.5; (O) 50 mM Tris-50 mM Mes-1 mM EDTA-1 mM Na2S04, pH 7.5. binds P, poorly (Tiefert et al, 1977; this paper) in contrast to mitochondrial F, (Penefsky, 1977) or bacterial F, (Pougeois et al, 1983). It is possible that the elution-centrifugation technique that was used to separate the bound and free [32P] P, is inappropriate, especially if [32P]P, dissociates very rapidly from its binding site.…”

Section: Resultsmentioning

confidence: 95%

“…Although the interaction of nucleotides with CF, has been studied in many laboratories [for a review, cf. Shavit (1980)], the binding of P, has not been investigated; to our knowledge, only mention was made that CF, does not bind P, (Tiefert et al, 1977). In fact, as shown here, CF, binds P, poorly.…”

mentioning

confidence: 74%

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Interaction of 4-azido-2-nitrophenyl phosphate, an inorganic phosphate photoreactive analog, with chloroplast coupling factor 1

Pougeois¹,

Lauquin²,

Vignais³

1983

Biochemistry

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4-Azido-2-nitrophenyl phosphate (ANPP), a photoreactive derivative of inorganic phosphate (Pj) [Lauquin, G., Pougeois, R., & Vignais, P. V. (1980) Biochemistry 19, 4620-4626], was used to study the P, binding site in purified spinach chloroplast coupling factor 1 (CFj). Upon photoirradiation with visible light, [32P]ANPP bound covalently to CF, and inactivated the enzyme. The labeling stoichiometry was measured in the presence of either EDTA, Mg2+, or Ca2+.

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Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 95%

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Interaction of 4-azido-2-nitrophenyl phosphate, an inorganic phosphate photoreactive analog, with chloroplast coupling factor 1

Pougeois¹,

Lauquin²,

Vignais³

1983

Biochemistry

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“…Our observation, that the Mg-ADP-fi complex is unstable, dissociating very rapidly upon removal of MgClt after the binding step, might explain earlier reported data on the poor binding of nucleotides to the isolated E. co/i &subunit [5,9] and of Pi to CFi [19,20], if also in these cases an unstable Mg-ligand-protein complex is formed. indeed, in all these reports MgClz, even when present during the incubation of the protein with the Iigand, was removed when separating the bound and free ligands.…”

supporting

confidence: 75%

Demonstration of two binding sites for ADP on the isolated β‐subunit of the Rhodospirillum rubrum R₁F₀F₁‐ATP synthase

Khananshvili

Gromet-Elhanan

1984

FEBS Letters

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Two ADP binding sites have been demonstrated on the reconstitutively active β‐subunit, that was removed from the Rhodospirillum rubrum membrane‐bound ATP synthase. One is a high affinity site (K d = 0.7 μM) that does not require MgCl2 and is unaffected by it. The second is a low affinity binding site (K d = 80 μM) that is absolutely dependent on MgCl2. For stable binding of ADP to this site, MgCl2 must be present not only during the binding step but also during the elution‐centrifugation step used to separate the β‐subunit bound [3H]ADP from the free ligand. When MgCl2 is removed together with the free ligand [3H]ADP dissociates very rapidly from this second binding site.

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“…This agent (193) is available in many countries outside of the United States at doses of 1.25-2.5 mg/d.…”

Section: Evidencementioning

confidence: 99%

Treatment of Symptoms of the Menopause: An Endocrine Society Clinical Practice Guideline

Stuenkel

Davis

Gompel

et al. 2015

The Journal of Clinical Endocrinology & Metabolism

680

419

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Menopausal hormone therapy (MHT) is the most effective treatment for vasomotor symptoms and other symptoms of the climacteric. Benefits may exceed risks for the majority of symptomatic postmenopausal women who are under age 60 or under 10 years since the onset of menopause. Health care professionals should individualize therapy based on clinical factors and patient preference. They should screen women before initiating MHT for cardiovascular and breast cancer risk and recommend the most appropriate therapy depending on risk/benefit considerations. Current evidence does not justify the use of MHT to prevent coronary heart disease, breast cancer, or dementia. Other options are available for those with vasomotor symptoms who prefer not to use MHT or who have contraindications because these patients should not use MHT. Low-dose vaginal estrogen and ospemifene provide effective therapy for the genitourinary syndrome of menopause, and vaginal moisturizers and lubricants are available for those not choosing hormonal therapy. All postmenopausal women should embrace appropriate lifestyle measures.

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Binding of adenine nucleotides and pyrophosphate by the purified coupling factor of photophosphorylation

Cited by 23 publications

References 22 publications

Interaction of 4-azido-2-nitrophenyl phosphate, an inorganic phosphate photoreactive analog, with chloroplast coupling factor 1

Interaction of 4-azido-2-nitrophenyl phosphate, an inorganic phosphate photoreactive analog, with chloroplast coupling factor 1

Demonstration of two binding sites for ADP on the isolated β‐subunit of the Rhodospirillum rubrum R₁F₀F₁‐ATP synthase

Treatment of Symptoms of the Menopause: An Endocrine Society Clinical Practice Guideline

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Binding of adenine nucleotides and pyrophosphate by the purified coupling factor of photophosphorylation

Cited by 23 publications

References 22 publications

Interaction of 4-azido-2-nitrophenyl phosphate, an inorganic phosphate photoreactive analog, with chloroplast coupling factor 1

Interaction of 4-azido-2-nitrophenyl phosphate, an inorganic phosphate photoreactive analog, with chloroplast coupling factor 1

Demonstration of two binding sites for ADP on the isolated β‐subunit of the Rhodospirillum rubrum R1F0F1‐ATP synthase

Treatment of Symptoms of the Menopause: An Endocrine Society Clinical Practice Guideline

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Demonstration of two binding sites for ADP on the isolated β‐subunit of the Rhodospirillum rubrum R₁F₀F₁‐ATP synthase