1998
DOI: 10.1074/jbc.273.48.31795
|View full text |Cite
|
Sign up to set email alerts
|

Binding of Anti-CD23 Monoclonal Antibody to the Leucine Zipper Motif of FcεRII/CD23 on B Cell Membrane Promotes Its Proteolytic Cleavage

Abstract: In the present study we have compared the binding of two monoclonal antibodies to CD23, EBVCS1 and mAb25, which recognize the stalk and the lectin domain, respectively, on the CD23 molecule. At 4°C, EBVCS1 binds to about 10% of the receptors recognized by mAb25 on the B cell surface. At 37°C, whereas mAb25 reaches its maximal binding within a few seconds, EB-VCS1 requires 60 min to bind to the same extent. Stabilization of the oligomeric structure of CD23 with IgE strongly affects in a dose-dependent fashion t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

4
21
0

Year Published

2001
2001
2016
2016

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 20 publications
(25 citation statements)
references
References 51 publications
4
21
0
Order By: Relevance
“…These data support the work presented by Cousin et al (41) who proposed that the mAb EBVCS1 enhanced cleavage of CD23 by interfering in oligomerization. This manuscript also supports the hypothesis of Cousin et al that any molecule that could destabilize CD23 trimers would cause an increase in IgE production.…”
Section: Figuresupporting
confidence: 82%
See 1 more Smart Citation
“…These data support the work presented by Cousin et al (41) who proposed that the mAb EBVCS1 enhanced cleavage of CD23 by interfering in oligomerization. This manuscript also supports the hypothesis of Cousin et al that any molecule that could destabilize CD23 trimers would cause an increase in IgE production.…”
Section: Figuresupporting
confidence: 82%
“…This same Ab was later shown to enhance cleavage of CD23. The enhancement of proteolysis by EBVCS-1 could be decreased by the addition of IgE (41).…”
Section: Figurementioning
confidence: 95%
“…The PCR products were sequenced to verify CD23b isoform-specific transcripts. To verify CD23 expression at the protein level, we blotted the cell lysates from Detroit 562, Calu-3, and A549 cells with EBVCS1 mAb, a specific IgG1 Ab for human CD23 (38,39). These airway epithelial cells expressed protein bands identical to 293T and intestinal T84 cells transfected with vectors expressing human CD23b cDNA in a Western blot (Fig.…”
Section: Human Respiratory Epithelial Cells Express Cd23mentioning
confidence: 99%
“…Therefore, it is important to better define the identities of the CD8 ϩ CD60 ϩ T cell subset (CD23 ϩ/Ϫ ) that help and/or suppress memory IgE responses in order that cells/ cytokine pathways might be accurately determined. Because CD23 is shed from cell surfaces (11)(12)(13) ϩ T cells; other cytokines were not studied). Interestingly, in the present studies, IL-13 was not required for induction of memory IgE responses.…”
Section: Discussionmentioning
confidence: 99%