Binding of mucin to water-soluble and surface-grafted boronate-containing polymers

Ivanov, Alexander E.; Solodukhina, N. М.; Nilsson, Lars; Nikitin, Maxim P.; Никитин, П. И.; Zubov, V.P.; Vikhrov, A. A.

doi:10.1134/s0965545x12010026

Cited by 16 publications

(12 citation statements)

References 41 publications

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“…Ivanov et al. reported a glass surface modified with a poly( N , N ‐dimethylacrylamide) brush having PBA pendant groups . The polymer brush underwent reversible conformational changes in response to ionic strength and pH, and its reversible binding property toward mucin as a glycoprotein.…”

Section: Nanoscale Hybrid and Composite Materialsmentioning

confidence: 99%

“…Seidler et al reported pH-responsive core-shell protein hybrids formed by proteins modified with BA moieties and poly (ethylene glycol) with a terminal salicyl-hydroxamate group. [24] Conjugation of a BA-modified protein and poly(ethylene glycol) led to a pH-responsive reversibly PEGylated protein that can be used for macromolecular therapeutics. Seidler et al also developed native protein hydrogels consisting of proteins modified with BAs and a linear or 4-armed poly(ethylene glycol) modified with salicyl-hydroxamate at termini.…”

Section: Biohybrid Materialsmentioning

confidence: 99%

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Cross‐Linking Building Blocks Using a “Boronate Bridge” to Build Functional Hybrid Materials

Nakahata

Sakai

2018

ChemNanoMat

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Boronic acids are not only a substrate for metal‐catalyzed cross‐coupling but also a molecular recognition tool. Especially, boronic acid‐diol or boronate‐diol interactions have been used to construct molecular‐scale self‐assemblies. This focus review summarizes recent researches using boronic acids as a bridge between similar or dissimilar (macro)molecules to build functional hybrid materials. Functional properties of boronic acid moieties such as molecular selectivity and stimuli‐responsiveness impart the materials with characteristic functions suitable for applications such as release systems, dynamic materials, and bioactive surfaces and scaffolds.

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Section: Nanoscale Hybrid and Composite Materialsmentioning

confidence: 99%

Section: Biohybrid Materialsmentioning

confidence: 99%

Cross‐Linking Building Blocks Using a “Boronate Bridge” to Build Functional Hybrid Materials

Nakahata

Sakai

2018

ChemNanoMat

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“…The procedures for monitoring of surface modifications by SCI, as well as the optimized immobilization protocols developed in this work, may also be useful for monitoring production of the glass biochips for the fluorescent assays. Moreover, the SCI biosensors are also compatible with a wide variety of partially transparent interface layers (polymer [37,38], inorganic materials, etc.) on the glass slips.…”

Section: B-chipsmentioning

confidence: 99%

Direct immunosensing by spectral correlation interferometry: assay characteristics versus antibody immobilization chemistry

et al. 2015

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A 3-channel biosensor based on spectral correlation interferometry (SCI) has been adapted for direct optical detection of antigens by measuring changes in thickness of a biolayer on functionalized glass slips employed as affordable single-use sensor chips. The instrument is insensitive to the bulk refractive index of a solution under test and provides signals in metrological units (pm or nm). Using real-time monitoring with the SCI, protocols for fabrication of sensor chips with different functional (epoxylated, carboxylated, and biotinylated) surfaces for antibody immobilization have been developed and optimized to minimize chip-to-chip variations and achieve better limit of detection (LOD), shorter assay time, and longer shelf life. The optimized coupling surfaces have been compared for detection of human serum albumin (HSA) used as a model agent of medical significance. The dynamic ranges for measuring the HSA concentration were 0.07-20, 0.12-30, and 0.25-10 μg/ml, and the assay durations were less than 20, 15, and 30 min for the epoxylated, carboxylated, and biotinylated chips, respectively. The advantages of each type of sensor chip have been shown, namely, the carboxylated chips feature the shortest assay time, the epoxylated ones demonstrate the best LOD, and the biotinylated chips exhibit the longest shelf life in an unprotected environment. The developed protocols of antibody immobilization can be used in different biosensors and assay techniques including those based on fluorescent, magnetic or plasmonic labels, etc. The SCI is well compatible with various partially transparent layers used in biosensing and with microarrays for multi-analyte detection.

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“…The progress of biochemical reactions was monitored in real time using an optical label free biosensor Picoscope® [16,17] based on the spectral correlation interferometry. The method allows employment of standard 100 μm thick microscopy cover slips as sensor chips.…”

Section: Reagentsmentioning

confidence: 99%

“…The approach provides a signal independent of the solution refractive index. The method of spectral correlation interferometry was successfully applied in various immunochemical studies [14], for elucidation of the mechanisms of drug action [15], in studies of protein interaction with novel polymers [16], and for the detection of a number of autoantibodies in human blood serum [17].…”

Section: Introductionmentioning

confidence: 99%

Detection of pyrethroids by spectral correlation interferometry

Burenin

Nikitin

Орлов

et al. 2013

Appl Biochem Microbiol

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A label free method based on spectral correlation interferometry has been developed for highly sensitive detection of pyrethroids by competitive immunoassay on the surface of sensor chips made of widely available microscopy glass cover slips. It is shown that the method allows independent optimization of each step of the sensor surface modification. This fact may be used to increase the efficiency of development of protocols for a wide spectrum of immunoassays that employ glass surface as a solid phase. Detection of 3 phenoxybenzoic acid, which is one of the most stable metabolites of a large number of pyrethroids, on the surface of the optimized sensor chips has been demonstrated on the level of 15 pg/ml. That is 50 times better than the sensitivity of the enzyme linked immunosorbent assay (ELISA).

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Binding of mucin to water-soluble and surface-grafted boronate-containing polymers

Cited by 16 publications

References 41 publications

Cross‐Linking Building Blocks Using a “Boronate Bridge” to Build Functional Hybrid Materials

Cross‐Linking Building Blocks Using a “Boronate Bridge” to Build Functional Hybrid Materials

Direct immunosensing by spectral correlation interferometry: assay characteristics versus antibody immobilization chemistry

Detection of pyrethroids by spectral correlation interferometry

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