Binding of serum osteocalcin to hydroxyapatite in Paget's disease of bone

Torres, R.; Piedra, Concepción de la; Rapado, A

doi:10.1016/0169-6009(91)90102-6

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…In that work, the authors employed for the deterrr ination of bAP, the same assay which was used in this paper (]lill's assay), but levels of BGP were determined by a policlonal antiserum. Independently of the type of assay used for the determination of BGP levels, this biochemical marker of bone turnover has not been found to be useful for the study of Paget's disease of the bone (Delmas, Demiaux, Malaya!, Chapuy and Meunier 1986; Torres, De Ia Piedra and Rapado 1991).…”

Section: Discussionmentioning

confidence: 99%

Lack of Correlation between Levels of Osteocalcin and Bone Alkaline Phosphatase in Healthy Control and Postmenopausal Osteoporotic Women

Diego

Martín²,

Piedra³

et al. 1995

Horm Metab Res

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The determination of bone alkaline phosphatase (bAR) by IRMA and the measure of intact osteocalcin (BGP) andlor different fragments by IRMA and/or RIA are new commercially available methods for the evaluation of osteoblast activity. The aim of this work was to study the possible correlation among serum bAR and levels of BGP measured by two different methods: an IRMA which only detects intact-BGP and a RIA which detects intact-BGP and carboxyterminal fragments (C-terminal BGP) in healthy postmenopausal control women and in a group of patients with postmenopausal osteoporosis. Serum samples from 42 consecutive osteoporotic postmenopausal women, aged 62 11 years, between 39-76, and 14 control women age matched, were drawn after an overnight fasting. Levels of total alkaline phosphatase (AR), bAR, intact BGP, and intact BGP and carboxy terminal fragments were measured. In both groups we found a significant linear correlation between the levels of AP and bAP (r = 0.79, p <0.001) and intact-BGP and C-terminal BGP (r = 0.97, p

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Section: Discussionmentioning

confidence: 99%

Lack of Correlation between Levels of Osteocalcin and Bone Alkaline Phosphatase in Healthy Control and Postmenopausal Osteoporotic Women

Diego

Martín²,

Piedra³

et al. 1995

Horm Metab Res

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“…Hydroxyapatite might be a more appropriate column packing for that purpose. In a previous work [27] ,we purified sera from healthy control subjects through a hydroxylapatite HPLC column and detected only a sharp peak of BGP eluting at 12 min at the beginning of the gradient (carboxylated BGP) and trace amounts of BGP (0·7% of the total BGP injected), which correspond to decarboxylated BGP, that did not bind to the column. This system did not allow us to distinguish between different forms of carboxylated BGP.…”

Section: Figurementioning

confidence: 99%

Immunoreactive osteocalcin forms in conditioned media from human osteoblast culture and in sera from healthy adult control subjects and patients with bone pathologies

Diego

Nacher

Rapado

et al. 1998

Eur J Clin Investigation

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“…Hierzu bietet die ebenfalls nicht vorhandene Konkordanz zwischen Osteocalcin und der Knochenfraktion der alkalischen Phosphatase beim Morbus Paget eine Parallele. Bei dieser Knochenerkrankung werden eine Dysregulation der Synthese und/oder abnorme Bindung des Osteocalcins an die alterierten Knochenstrukturen als Ursachen für die divergenten Veränderungen dieser beiden Parameter des Knochenstoffwechsels angesehen (16,17 …”

Section: Ergebnisse Und Diskussionunclassified

Osteocalcin und die Knochenfraktion der alkalischen Phosphatase im Serum nierentransplantierter Patienten

John¹,

Jung²,

Lippert³

1992

LaboratoriumsMedizin

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Osteocalcin and the bone-specific fraction of alkaline phosphatase were determined in sera of 50 kidney transplant recipients. Compared to the upper limit of the reference intervals (97.5 percentiles), the bone-specific alkaline phosphatase was more often increased than osteocalcin (25 vs. 5). There was only a weak correlation between the two analytes (r s = 0.306). it was concluded that osteocalcin and the bone fraction of alkaline phosphatase do not identically reflect the bone formation in patients after kidney transplantation. The determination of bone fraction of alkaline phosphatase was recommended to follow up the bone metabolism in these patients.

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Binding of serum osteocalcin to hydroxyapatite in Paget's disease of bone

Cited by 5 publications

References 15 publications

Lack of Correlation between Levels of Osteocalcin and Bone Alkaline Phosphatase in Healthy Control and Postmenopausal Osteoporotic Women

Lack of Correlation between Levels of Osteocalcin and Bone Alkaline Phosphatase in Healthy Control and Postmenopausal Osteoporotic Women

Immunoreactive osteocalcin forms in conditioned media from human osteoblast culture and in sera from healthy adult control subjects and patients with bone pathologies

Osteocalcin und die Knochenfraktion der alkalischen Phosphatase im Serum nierentransplantierter Patienten

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