Binding of Water‐Soluble CdSe Quantum Dots with Human Serum Albumin: Further Studies into their Effects on Dietary Polyphenol Binding and Sensing of Antibiotic Lomefloxacin

Abstract

Synthesis of surface modified quantum dots (QDs) having potential biological activities are great important in biomedicinal chemistry. Here, we have synthesized mercapto propanoic acid (MPA) and thiolactic acid (TLA) coated water soluble cadmium selenide (CdSe) QDs having biological activity. Dispersion of QDs into a biological fluid can form protein corona. Hence, the binding of CdSe QDs with the carrier protein, human serum albumin (HSA) have been executed using spectroscopic methods. The binding affinity order of CdSe QDs-HSA complexes were found in the order of 10 5 M À 1 at 298 K. CdSe QDs are also affects the binding affinities of dietary polyphenols (naringin: NG and naringenin: NRG) towards HSA. The synthesized CdSe QDs was employed to sensing of antibiotic lomefloxacin by fluorescence quenching mechanism. The detection limit was found 0.70-1.83 μg mL À 1 . The CdSe QDs also demonstrate the detection of lomefloxacin under uv light based on color change of the solutions.

Interaction properties of biosynthesized cadmium sulphide quantum dots with human serum albumin: further investigation of antibacterial activities and sensing applications

“…In this context, many articles have been reported based on protein–QD interactions using multispectroscopic approaches. [ 31–33 ] However, studies of green strategies for the synthesis of CdS QDs remain elusive.…”

Formation of ZnS quantum dots using green tea extract: applications to protein binding, bio-sensing, anti-bacterial and cell cytotoxicity studies

“…The results obtained in this case are optimistic, which was not observed in chemically synthesized cadmium-based QDs (where the binding was affected negatively). 15,29,70 3.4. Thermodynamic parameters for HSA-ligand associations…”

“…11 QDs have emerged as chemo-and biosensors due to their high selectivity and sensitivity towards inorganic ions 12 (i.e., cations and anions), biomolecules 13,14 (e.g., glycoprotein, glucose, bilirubin, etc. ), drug molecules 15 (e.g., antibiotics), and pesticides. 16 Among the different types of QD, metal chalcogenide QDs particularly zinc sulphide (ZnS) QDs have received much more attention due to their outstanding properties and versatile applications.…”

“…As model carrier proteins for different types of biophysical and physicochemical research, serum albumins have been well-studied. Several articles have been published in this context, 15,29,39 but green-tea-constituent-capped ZnS QD interactions have remained elusive. The type and degree of interactions with the proteins may be impacted by the green tea constituents adsorbed on the surface of the ZnS QDs.…”

“…, glycoprotein, glucose, bilirubin, etc. ), drug molecules 15 ( e.g. , antibiotics), and pesticides.…”