Binding saturation of n-alkyl trimethylammonium bromide on bovine serum albumin

Determination of the Binding Isotherm and Size of the Bovine Serum Albumin-Sodium Dodecyl Sulfate Complex by Diffusion-Ordered 2D NMR

“…Therefore, Dmic can be determined from Dm0n and the measured average value only if the fraction of monomer (Pmon) is known. This fraction was computed as a function of Q, the total concentration of SDS, by means of the mass-action model20 Assuming monodispersity of the micelles, the micellization constant Ka is given by Ka ¿ log[^-J (5) with the constants log Ka = 230, a = 64, and b = 46.7 reported by Moroi.20 4. The value of Dm¡c obtained in part 3 does not accurately represent the diffusion coefficient of micelles in the presence of the protein because the solution environment is altered.…”

Interactions of Globular Proteins with Surfactants Studied with Fluorescence Probe Methods

“…Turro et al used pyrene excimer formation to probe the aggregates; we used pyrene with different quenchers and also tested the simpler static method. The data regarding the interaction of cationic and nonionic surfactants with BSA are scarce. ,− We also tried the application of the method to investigate BSA−CTAB (a cationic surfactant), BSA−C 12 E 8 (a nonionic surfactant), and Lys−SDS systems. Some of the solutions were examined with the cryo-TEM method.…”

“…The data regarding the interaction of cationic and nonionic surfactants with BSA are scarce. 36,[39][40][41][42][43][44] We also tried the application of the method to investigate BSA-CTAB (a cationic surfactant), BSA-C 12 E 8 (a nonionic surfactant), and Lys-SDS systems. Some of the solutions were examined with the cryo-TEM method.…”

2 Neutron reflection studies of interactions between surfactants and proteins at interfaces

“…The lack of progress in understanding interfacial interactions is in contrast to the advances made in the characterisation of protein/surfactant systems in bulk solution. Techniques such as dialysis, ion selective electrodes, NMR, circular dichroism (CD), infrared and small angle neutron scattering (SANS) [12][13][14][15][16][17][18][19][20][21][22] have provided valuable structural and compositional information for a variety of bulk systems. None of these techniques, however, is either sensitive to interfacial structure or able to distinguish protein from surfactant within the interfacial region.…”