Binding structure of the leucine aminopeptidase inhibitor microginin FR1

Kraft, Manuel; Schleberger, Christian; Weckesser, Jürgen; Schulz, Georg E.

doi:10.1016/j.febslet.2006.11.060

Cited by 24 publications

(27 citation statements)

References 35 publications

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“…The ppLAP-bestatin crystal structure showed excellent density for the bestatin inhibitor in the active site with bestatin, 11,18 and the bestatin derivative microginin FR1. 19 A schematic overview of the polar interactions of bestatin with ppLAP is given in Fig. 4.…”

Section: Structure Of Bestatin-bound Pplapmentioning

confidence: 99%

Crystal Structure of the Leucine Aminopeptidase from Pseudomonas putida Reveals the Molecular Basis for its Enantioselectivity and Broad Substrate Specificity

Kale

Pijning

Sonke

et al. 2010

Journal of Molecular Biology

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Section: Structure Of Bestatin-bound Pplapmentioning

confidence: 99%

Crystal Structure of the Leucine Aminopeptidase from Pseudomonas putida Reveals the Molecular Basis for its Enantioselectivity and Broad Substrate Specificity

Kale

Pijning

Sonke

et al. 2010

Journal of Molecular Biology

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“…This is common for all family 5 APNs. A role similar to that suggested for inactive proteases seems unlikely because aminopeptidase inhibitors have not been reported from plants, although aminopeptidase inhibitory peptides have been isolated from bacteria (Kraft et al ., 2006).…”

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confidence: 99%

Expressed sequence tags from the midgut of Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae)

Simpson

Newcomb

Gatehouse

et al. 2007

Insect Molecular Biology

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The midgut is a key tissue in insect science. Physiological roles include digestion and peritrophic membrane function, as well as being an important target for insecticides. We used an expressed sequence tag (EST) approach to identify candidate genes and gene families involved in these processes in the light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae). Two cDNA libraries were constructed from dissected midgut of third to fifth instar larvae. Clustering analysis of 6416 expressed sequence tags produced 1178 tentative unique genes comprising 725 tentative contigs and 453 singletons. The sequences show similar codon usage to sequences from other lepidopterans, a Kozak consensus sequence similar to Drosophila and single nucleotide polymorphisms (SNPs) were detected at a frequency of 1.35/kb. The identity of the most common Interpro families correlates well with major known functions of the midgut. Phylogenetic analysis was conducted on representative sequences from selected multigene families. Gene families include a broad range of digestive proteases, lipases and carbohydrases that appear to have degradative capacity against the major food components found in leaves, the diet of these larvae; and carboxylesterases, glutathione-S-transferases and cytochrome P450 monooxygenases, potentially involved in xenobiotic degradation. Two of the larger multigene families, serine proteases and lipases, expressed a high proportion of genes that are likely to be catalytically inactive.

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“…The structure N-Me-Tyr-Tyr at the C-terminus seemed to be crucial for the inhibition of angiotensin-converting enzyme (ACE) (Ishida et al 2000). The inhibitory activity of some microginins for ACE was considered for the production of blood pressure-decreasing drugs (Kraft et al 2006). However, some microginins did not show any inhibition activity against trypsin, chymotrypsin, papain, elastase or protein phosphatase 1A (Ishida et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Determination of the toxicity of the freshwater cyanobacterium Woronichinia naegeliana (Unger) Elenkin

Bober

Białçzyk

2017

J Appl Phycol

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Cyanobacterial blooms are undesirable for ecological and health reasons. While Woronichinia naegeliana is a cyanobacterial species that appears frequently in freshwater, information about it is limited. An evaluation of its toxicity was conducted via tests based on the crustaceans Thamnocephalus platyurus and Daphnia pulex. The greatest effect of the aqueous extract obtained from W. naegeliana cells was observed for T. platyurus. The denoted semi-lethal concentration (LC50) after 24 h of exposure was 0.99 mg of dry weight (d.w.) mL−1. A lower toxicity was displayed for D. pulex, although it grew with time. Among the 18 fractions separated from cyanobacterial extract, only one containing the microginin FR3 (MG-FR3) displayed biological activeness against T. platyurus. The remaining products synthesized by W. naegeliana displayed an absence or a low level of toxicity making it impossible to determine the LC50 value. Detailed research revealed that MG-FR3 did not affect the activity of enzymes such as trypsin, chymotrypsin, elastase and thrombin, which indicates another mode of action. The results demonstrated that blooms of W. naegeliana showed toxic activity towards invertebrate zooplankton.

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Binding structure of the leucine aminopeptidase inhibitor microginin FR1

Cited by 24 publications

References 35 publications

Crystal Structure of the Leucine Aminopeptidase from Pseudomonas putida Reveals the Molecular Basis for its Enantioselectivity and Broad Substrate Specificity

Crystal Structure of the Leucine Aminopeptidase from Pseudomonas putida Reveals the Molecular Basis for its Enantioselectivity and Broad Substrate Specificity

Expressed sequence tags from the midgut of Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae)

Determination of the toxicity of the freshwater cyanobacterium Woronichinia naegeliana (Unger) Elenkin

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