Binding the Tobacco Mosaic Virus to Inorganic Surfaces

Knez, Mato; Sumser, Martin; Bittner, Alexander M.; Wege, Christina; Jeske, Holger; Hoffmann, D. M. P.; Kuhnke, Klaus; Kern, Klaus

doi:10.1021/la035425o

Cited by 105 publications

(116 citation statements)

References 43 publications

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“…The measured height of Pt-TMV2cys from the section analysis is approximately 12 nm, which is similar to the apparent height of wt TMV on a gold[111] substrate. 36 …”

Section: Resultsmentioning

confidence: 99%

Deposition of Platinum Clusters on Surface-Modified Tobacco Mosaic Virus

Lee¹,

Choi²,

Royston³

et al. 2006

J. Nanosci. Nanotech.

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Nanoscaled Pt conductors were prepared from genetically engineered Tobacco mosaic virus (TMV) templates through Pt cluster deposition on the outer surface of the TMV. Pt clusters were synthesized and deposited on the engineered TMV with surface-exposed cysteine via the in situ mineralization of hexachloroplatinate anions. This deposition was driven by the specific binding between thiols and the solid metal clusters. In addition, Pt-thiolate adducts are suggested to form on the engineered TMV in aqueous solutions that work as nucleation sites for the formation of the Pt clusters. The specific binding between Pt clusters and the engineered TMV template was investigated using UV/vis spectrophotometry and quartz crystal microbalance (QCM) analysis. The electric conductance of Pt-deposited TMV was greater than that of the uncoated TMV virion particles. This result suggests the application of metal cluster-deposited engineered TMV in future electrical devices such as rapid response sensors.

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“…The measured height of Pt-TMV2cys from the section analysis is approximately 12 nm, which is similar to the apparent height of wt TMV on a gold[111] substrate. 36 …”

Section: Resultsmentioning

confidence: 99%

Deposition of Platinum Clusters on Surface-Modified Tobacco Mosaic Virus

Lee¹,

Choi²,

Royston³

et al. 2006

J. Nanosci. Nanotech.

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“…AFM experiments showed that it is possible to image TMV at solid-air and solid-liquid interfaces on welldefined substrates such as mica and Au (111). 333 M13 Bacteriophage. Another example of the use of viruses for the assembly of inorganic materials has been given by Belcher and co-workers.…”

Section: Rod-shaped Virusesmentioning

confidence: 99%

Self-Assembled Nanoreactors

et al. 2005

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“…Second, the TMV is an extensively studied virus by different techniques, and hence its structure, mass and mechanical properties are well-established. TMV is rod-shaped and is formed from approximately 2130 identical protein subunits wound in a 300-nm long helix with a diameter of 18 nm [16][17] . A central hollow cylindrical core holds the viral genome-a 6395-nucleotide strand of RNA.…”

mentioning

confidence: 99%

Physics of Nanomechanical Spectrometry of Viruses

Ruz¹,

Tamayo²,

Pini³

et al. 2014

Sci Rep

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There is an emerging need of nanotools able to quantify the mechanical properties of single biological entities. A promising approach is the measurement of the shifts of the resonant frequencies of ultrathin cantilevers induced by the adsorption of the studied biological systems. Here, we present a detailed theoretical analysis to calculate the resonance frequency shift induced by the mechanical stiffness of viral nanotubes. The model accounts for the high surface-to-volume ratio featured by single biological entities, the shape anisotropy and the interfacial adhesion. The model is applied to the case in which tobacco mosaic virus is randomly delivered to a silicon nitride cantilever. The theoretical framework opens the door to a novel paradigm for biological spectrometry as well as for measuring the Young's modulus of biological systems with minimal strains.I t is increasingly evident the intimate link between the mechanical properties of biological systems and its role in fundamental biological processes and disease 1 . This link spans from the molecular scale to the tissue scale. For example, the elasticity of cells has become a reliable indicator of cell transformation into cancerous or metastatic cells [2][3] . Similarly, recent reports have demonstrated the biological relevance of the mechanical properties of viruses. Viruses are able to dynamically modulate their mechanical properties in response to external forces, so as to withstand those forces or to ease cell infection 4 . For instance, in the human immunodeficiency and murine leukemia viruses, the stiffness largely decreases during the maturation process, acting as a mechanical switch for the infection process 5 . Strikingly, a single point mutation in the capsid protein of some viruses can significantly change their elasticity 6 . It is therefore fundamental the development of nanotools that enable the accurate quantification of the nanomechanical properties of single biological entities with high throughput. These tools can provide new insights on how the structural conformation, biological function, and mechanical properties of biomolecules and their hierarchical assemblies are related each other. The most prominent method to measure the mechanical properties of biological entities has been so far nanoindentation with the cantilever/tip assembly of an atomic force microscope (AFM) 7 . However, a number of challenges exist with the AFM for the quantification of the mechanical properties. Mainly, the nanoindentation curves strongly depend on the nanometer-scale geometry of the tip/sample contact, which in most of the cases cannot be controlled. Other difficulties include the contribution of the underlying substrate, the effect of adhesion, non-linear loading and the lack of accurate theoretical models.We envisage a novel biological spectrometry technique based on the measurement of several vibration modes of ultrathin micro-and nanocantilevers for the identification of adsorbed biomolecules and biological systems by two coordinates: the mass 8-11 an...

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Binding the Tobacco Mosaic Virus to Inorganic Surfaces

Cited by 105 publications

References 43 publications

Deposition of Platinum Clusters on Surface-Modified Tobacco Mosaic Virus

Deposition of Platinum Clusters on Surface-Modified Tobacco Mosaic Virus

Self-Assembled Nanoreactors

Physics of Nanomechanical Spectrometry of Viruses

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