Bio-affinity mediated immobilization of lipase onto magnetic cellulose nanospheres for high yield biodiesel in one time addition of methanol

Bandikari, Ramesh; Qian, Jiaxin; Baskaran, R.; Liu, Ziduo; Wu, Gaobing

doi:10.1016/j.biortech.2017.09.156

Cited by 37 publications

(10 citation statements)

References 44 publications

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“…The Degree of substitution (GS) obtained was 18.00 – that is, 100 anhydroglucopyranosic units; 18.00 cellulose hydroxyls were replaced by the xanthate group for every 100 anhydroglucopyranosic units, and 22 was the maximum number of groups that could be replaced 37 . Other supports derived from cellulose have already been used to immobilize lipases for the production of biodiesel, such as magnetic cellulose nanocrystals, 38 cellulosic polyurethane, 39 magnetic cellulose nanospheres, 40 and modified polyporous magnetic cellulose 41 …”

Section: Resultsmentioning

confidence: 99%

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Rial

Freitas

Cavalheiro

et al. 2021

Biofuels Bioprod Bioref

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This work investigated the best reaction conditions to obtain biodiesel from soybean oil using Candida rugosa lipase (CRL) immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel) support. The alcohols used were methanol and ethanol and the molar ratio used was 1:4 (n/n). The CRL immobilized on NBXCel support showed greater catalytic activity at pH 7.00 and at 45 °C. Thus, 23 experiments were carried out at this pH and at this temperature, varying the reaction time, type of alcohol, and amount of immobilized CRL to achieve a conversion equal to or greater than 96.5% as recommended by EN 14103. The highest conversions of methyl biodiesel were achieved using 4.31%mCRL/mNBXCel and with 3.89%mCRL/mNBXCel. Ethyl biodiesel was achieved at the highest conversion using 5.18% and 4.76%mCRL/mNBXCel. The results were explored with regression analysis to identify the best reaction conditions to obtain a good conversion, and the response surface graphs were plotted to visualize other reaction conditions. We also evaluated the number of reuse cycles of CRL immobilized on NBXCel support, to lower biodiesel production cost, and after 15 cycles of reuse the conversions obtained were above 50%, demonstrating the efficiency of CRL immobilized on the NBXCel support in catalyzing the synthesis of biodiesel. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Resultsmentioning

confidence: 99%

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Rial

Freitas

Cavalheiro

et al. 2021

Biofuels Bioprod Bioref

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“…The disadvantages of processes catalyzed by strong acids are high reaction temperatures, difficult separation of reaction media, low yields and environmental concerns (Cui et al, 2020). However, the enzymatic route has proved attractive for the production of these esters because it is a process with low energy consumption, high productivity, and good stability compared to organic solvents (Bandikari et al, 2018), in addition to having broad substrate specificity and exhibiting high enantioselectivity (Benamia et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Candida rugosa lipase immobilized on hydrophobic support Accurel MP 1000 in the synthesis of emollient esters

et al. 2021

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“…The immobilized enzyme can be quickly separated from the reaction system by an external magnetic field and can therefore be recycled. Furthermore, a 3-aminopropyltriethoxysilane (APTES) functionalized magnetic carrier is a rigid multi-functional cross-linking agent that reduces the fluidity of the lipase lip and thus produces a stable open form of immobilized lipase [28,29,30].…”

Section: Introductionmentioning

confidence: 99%

CALB Immobilized onto Magnetic Nanoparticles for Efficient Kinetic Resolution of Racemic Secondary Alcohols: Long-Term Stability and Reusability

et al. 2019

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In this study, an immobilization strategy for magnetic cross-linking enzyme aggregates of lipase B from Candida antarctica (CALB) was developed and investigated. Magnetic particles were prepared by conventional co-precipitation. The magnetic nanoparticles were modified with 3-aminopropyltriethoxysilane (APTES) to obtain surface amino-functionalized magnetic nanoparticles (APTES–Fe3O4) as immobilization materials. Glutaraldehyde was used as a crosslinker to covalently bind CALB to APTES–Fe3O4. The optimal conditions of immobilization of lipase and resolution of racemic 1-phenylethanol were investigated. Under optimal conditions, esters could be obtained with conversion of 50%, enantiomeric excess of product (eep) > 99%, enantiomeric excess of substrate (ees) > 99%, and enantiomeric ratio (E) > 1000. The magnetic CALB CLEAs were successfully used for enzymatic kinetic resolution of fifteen secondary alcohols. Compared with Novozym 435, the magnetic CALB CLEAs exhibited a better enantioselectivity for most substrates. The conversion was still greater than 49% after the magnetic CALB CLEAs had been reused 10 times in a 48 h reaction cycle; both ees and eep were close to 99%. Furthermore, there was little decrease in catalytic activity and enantioselectivity after being stored at −20 °C for 90 days.

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Bio-affinity mediated immobilization of lipase onto magnetic cellulose nanospheres for high yield biodiesel in one time addition of methanol

Cited by 37 publications

References 44 publications

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p‐nitrobenzyl cellulose xanthate (NBXCel)

Candida rugosa lipase immobilized on hydrophobic support Accurel MP 1000 in the synthesis of emollient esters

CALB Immobilized onto Magnetic Nanoparticles for Efficient Kinetic Resolution of Racemic Secondary Alcohols: Long-Term Stability and Reusability

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