Bio‐Click Chemistry: Enzymatic Functionalization of PEGylated Capsules for Targeting Applications

Leung, Melissa K. M.; Hagemeyer, Christoph E.; Johnston, Andrew D.; Gonzales, Catalina; Kamphuis, Marloes M. J.; Ardipradja, Katie; Such, Georgina K.; Peter, Karlheinz; Caruso, Frank

doi:10.1002/ange.201203612

Cited by 27 publications

(20 citation statements)

References 28 publications

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“…[20] The same approach was exploited for ligand functionalization of pegylated capsules. [21] In principle, this bimodular orthogonal bioreaction could present two important advantages when the homing ligand is a short peptide (i.e., 5-30 aa): 1) the peptide is separated from the nanoparticle surface by a protein spacer, which prevents undesired interactions and thus optimizes the ligand availability for molecular recognition; 2) the introduction of globular proteins (i.e., the reacting enzyme) enhances the solubility of the nanoconstruct.…”

mentioning

confidence: 99%

Orientation‐Controlled Conjugation of Haloalkane Dehalogenase Fused Homing Peptides to Multifunctional Nanoparticles for the Specific Recognition of Cancer Cells

Mazzucchelli¹,

Colombo²,

Verderio³

et al. 2013

Angew Chem Int Ed

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mentioning

confidence: 99%

Orientation‐Controlled Conjugation of Haloalkane Dehalogenase Fused Homing Peptides to Multifunctional Nanoparticles for the Specific Recognition of Cancer Cells

Mazzucchelli¹,

Colombo²,

Verderio³

et al. 2013

Angew Chem Int Ed

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“…Importantly, the antibody-coupling densities for sortase-mediated functionalization were almost similar to that of CuAAC reactions, but provided a means to generate bioconjugates with controlled and defined orientation of the antibody. [64] …”

Section: Enzyme-based Bioconjugationmentioning

confidence: 99%

“…Reproduced with permission. [64] www.advancedsciencenews.com www. Significant tumor reduction with tumor vasculature disruption was spotted in vivo for the targeted system.…”

Section: Synthesis Of Antibody Fragment-nanomaterials Conjugatesmentioning

confidence: 99%

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Sivaram

Wardiana

Howard

et al. 2017

Adv Healthcare Materials

101

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Targeted nanomedicines have significantly changed the way new therapeutics are designed to treat disease. Central to successful therapeutics is the ability to control the dynamics of protein–nanomaterial interactions to enhance the therapeutic effect of the nanomedicine. The aim of this review is to illustrate the diversity and versatility of the conjugation approaches involved in the synthesis of antibody–nanoparticle conjugates, and highlight significant new advances in the field of bioconjugation. Such nanomedicines have found utility as both advanced therapeutic agents, as well as more complex imaging contrast agents that can provide both anatomical and functional information of diseased tissue. While such conjugates show significant promise as next generation targeted nanomedicines, it is recognized that there are in fact no clinically approved targeted therapeutics on the market. This fact is reflected upon within this review, and attempts are made to draw some reasoning from the complexities associated with the bioconjugation chemistry approaches that are typically utilized. Present trends, as well as future directions of next generation targeted nanomedicines are also discussed.

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“…The capsules showed a high specificity towards platelets. [97] The site-specific modification of liposomes was performed by Guo et al who prepared vesicles using phospholipids that carry a diglycine motif. An eGFP containing the SrtA recognition sequence was subsequently successfully coupled to the liposomes as shown by fluorescence imaging.…”

Section: Peptide and Protein Cyclizationmentioning

confidence: 99%

Sortagging: A Robust and Efficient Chemoenzymatic Ligation Strategy

Ritzefeld

2014

Chemistry A European J

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Bioorthogonal, chemoselective ligation methods are an essential part of the tools utilized to investigate biochemical pathways. Specifically enzymatic approaches are valuable methods in this context due to the inherent specificity of the deployed enzymes and the mild conditions of the modification reactions. One of the most common strategies is based on the transpeptidation catalyzed by sortase A derived from Staphylococcus aureus. The procedure is well established and a wide variety of applications have been published to date. Here, implementations of sortase A, which range from protein labeling using fluorescence dyes and the preparation of cyclic proteins to the modification of entire cells, are summarized. Furthermore, there is a focus on the optimization approaches established to solve the drawbacks of sortase-mediated transpeptidation.

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Bio‐Click Chemistry: Enzymatic Functionalization of PEGylated Capsules for Targeting Applications

Cited by 27 publications

References 28 publications

Orientation‐Controlled Conjugation of Haloalkane Dehalogenase Fused Homing Peptides to Multifunctional Nanoparticles for the Specific Recognition of Cancer Cells

Orientation‐Controlled Conjugation of Haloalkane Dehalogenase Fused Homing Peptides to Multifunctional Nanoparticles for the Specific Recognition of Cancer Cells

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Sortagging: A Robust and Efficient Chemoenzymatic Ligation Strategy

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