Bio-degradation of Bisphenol A by Pseudomonas aeruginosa PAb1 isolated from effluent of thermal paper industry: Kinetic modeling and process optimization

Abstract

A bacterium isolated from effluent of thermal paper industry and identified as Pseudomonas aeruginosa (PAb1) based on 16SrRNA gene sequence analysis which could grow on basal mineral salt medium upon bisphenol A, which functions as an exclusive carbon source. Physicochemical variables of thermal paper industry effluent noted were significantly greater than the typical limit due to pollution of the acquiring water systems. The mathematic kinetic models like Monod, Moser and Tesier models were applied for batch fermentation of bisphenol A degradation in basal salt medium and the half saturation coefficient (K S) and the regression coefficient R 2 using Monad, Moser and Tesier kinetic models registered as 9.947 g/L, 12.46 g/L and 14.14 g/L and 0.91, 0.94 and 0.84 respectively. Besides, the utmost specific growth rate mmax was witnessed as 0.841 h À1 for the P. aeruginosa (PAb1) regarding BPA degradation. Metabolic intermediates like phenol, acetophenone, and hydroquinone and p-hydroxybenzoic acid were also determined through the degradation process by GC-MS. The metabolic pathway of BPA degradation by the bacterial isolates was also designed in today's analysis. A probabilistic statistical model originated using Box-Behnken response surface methodology and process variables were optimized by nonlinear optimization.

Biodegradation and detoxification of bisphenol A by bacteria isolated from desert soils

“…KU3 showed, respectively, 78 ± 4%, 81 ± 3% and 74 ± 2% efficiency in degrading 1000 ppm BPA within 12 days. Vijayalakshmi et al (2018) reported that Pseudomonas aeruginosa (PAb1) is able to degrade 16.30 mM BPA at optimal conditions (pH: 6.94, temperature: 32.61). For P. putida specie, strain G320 seems to be more performant in BPA biodegradation compared to KU5 strain described by Kang and Kondo (2002) which degraded about 1 mg L −1 BPA within 10 days.…”

“…Thus, BPA removal from the natural environment is an increasing worldwide concern. Several BPA transforming or/and degrading organisms such as bacteria, fungi, algae and plants were effectively used to remove BPA from the environment (Michalowicz 2014;Zhang et al 2013;Kamaraj et al 2014;Mita et al 2015;Vijayalakshmi et al 2018). More and more BPA-degrading bacteria have been isolated from water, sediment, soil and wastewater treatment plants.…”

“…They included both Gram-negative and Gram-positive strains (Das et al 2018;Ebenezer and Ki 2016;Hou et al 2014;Fouda 2015;Zhang et al 2013). Different BPA degradation pathways have been proposed in the literature according to the intermediates detected during the degradation process for specific bacterial strains (Li et al 2012;Zhang et al 2013;Fischer et al 2010;Vijayalakshmi et al 2018). Until now, most BPA biodegradation studies were conducted by single-strain or bacterial consortia isolated from sediments or aquatic environments (Peng et al 2015;Kamaraj et al 2014;Eio et al 2014;Yang et al 2014;Li et al 2012;Kang and Kondo 2002;Moussavi and Haddad 2019) but no studies were made for deserts and arid area's bacteria.…”

Optimization and extraction of pharmaceutical micro-pollutant - norfloxacin using emulsion liquid membranes

“…Three factors namely, NaOH concentration (0.25-0.75 M, X 1 ), initial norfloxacin concentration (25-75 mg/L, X 2 ) and saponin concentration (0.01-0.03 g/100 mL, X 3 ) were considered to analyse the extraction efficiency of norfloxacin from aqueous solution. In order to investigate the combined effects of the selected variable on percentage norfloxacin removal the experiments were carried out according to the BBD [26][27][28][29]. The results for BBD are shown in Table 1.…”

“…The predicted R 2 (0.9214) and adjusted R 2 (0.9323) were in practical tuning with each other. Greater than 4 of adequate precision value specified an adequate signal [28]. Hence, the predicted quadratic models could navigate the design space to understand the factors interaction.…”