Bio-Fabrication of Human Amniotic Membrane Zinc Oxide Nanoparticles and the Wet/Dry HAM Dressing Membrane for Wound Healing

Ramasamy, Palaniappan; Krishnakumar, Ramachandran; Rekha, Ravichandran; Vaseeharan, Baskaralingam; Saraswathi, K; Raj, M. A. Lourdu Antony; Hanna, R.E.B.; Brennan, Gerard; Dayanithi, Govindan; Vijayakumar, Sekar

doi:10.3389/fbioe.2021.695710

Cited by 13 publications

(9 citation statements)

References 61 publications

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“…Twenty-one days after surgery, the development of functional epithelium on the conjunctival surface was complete, and the HAAM appeared to dissolve and disappear. One month later, the wound in the operative area was completely healed [ 16 ]. Additionally, the full-thickness auricular skin defects [ 20 ] and bone/dental regeneration [ 83 ] verified the above conclusions, meaning that HAAM is an ideal biological dressing for facial wounds.…”

Section: Clinical Applications Of Haammentioning

confidence: 99%

Human Acellular Amniotic Membrane as Skin Substitute and Biological Scaffold: A Review of Its Preparation, Preclinical Research, and Clinical Application

Li,

An,

Deng

et al. 2023

Pharmaceutics

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Human acellular amniotic membrane (HAAM) has emerged as a promising tool in the field of regenerative medicine, particularly for wound healing and tissue regeneration. HAAM provides a natural biological scaffold with low immunogenicity and good anti-infective and anti-scarring results. Despite its potential, the clinic application of HAAM faces challenges, particularly with respect to the preparation methods and its low mechanical strength. This review provides a comprehensive overview of HAAM, covering its preparation, sterilization, preclinical research, and clinical applications. This review also discusses promising decellularization and sterilization methods, such as Supercritical Carbon Dioxide (SC-CO2), and the need for further research into the regenerative mechanisms of HAAM. In addition, we discuss the potential of HAAM as a skin dressing and cell delivery system in preclinical research and clinical applications. Both the safety and effectiveness of HAAM have been validated by extensive research, which provides a robust foundation for its clinical application.

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Section: Clinical Applications Of Haammentioning

confidence: 99%

Human Acellular Amniotic Membrane as Skin Substitute and Biological Scaffold: A Review of Its Preparation, Preclinical Research, and Clinical Application

Li,

An,

Deng

et al. 2023

Pharmaceutics

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“…This produced composite had strong antibacterial properties on the formation and thickness of gram-negative (S. aureus, S. mutans, E. faecalis, and L. fusiformis) and grampositive (S. sonnei, P. aeruginosa, P. Vulgaris, and C. freundii) bacterial biofilms. 62 Other amniotic membrane-derived tissue engineered constructs Amnion, amnion homogenate, amnion powder, or lyophilized amnion has been combined with other natural or synthetic materials to reach a desirable scaffold for tissue engineering in various studies. Hybrid scaffolds are widely evaluated in tissue engineering.…”

Section: Amniotic Membrane-based Nanoparticlesmentioning

confidence: 99%

“…This produced composite had strong antibacterial properties on the formation and thickness of gram-negative ( S. aureus, S. mutans, E. faecalis, and L. fusiformis ) and gram-positive ( S. sonnei, P. aeruginosa, P. Vulgaris, and C. freundii ) bacterial biofilms. 62…”

Section: Introductionmentioning

confidence: 99%

Human amniotic membrane as a multifunctional biomaterial: recent advances and applications

et al. 2022

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The developing fetus is wrapped by a human amniotic membrane or amnion. Amnion is a promising human tissue allograft in clinical application because of its chemical composition, collagen-based, and mechanical properties of the extracellular matrix. In addition, amnion contains cells and growth factors; therefore, meets the essential parameters of tissue engineering. No donor morbidity, easy processing and storage, fewer ethical issue, anti-inflammatory, antioxidant, antibacterial, and non-immunogenic properties are other advantages of amnion usage. For these reasons, amnion can resolve some bottlenecks in the regenerative medicine issues such as tissue engineering and cell therapy. Over the last decades, biomedical applications of amnion have evolved from a simple sheet for skin or cornea repair to high-technology applications such as amnion nanocomposite, powder, or hydrogel for the regeneration of cartilage, muscle, tendon, and heart. Furthermore, amnion has anticancer as well as drug/cell delivery capacity. This review highlights various ancient and new applications of amnion in research and clinical applications, from regenerative medicine to cancer therapy, focusing on articles published during the last decade that also revealed information regarding amnion-based products. Challenges and future perspectives of the amnion in regenerative medicine are also discussed.

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“…Currently, various kinds of antibacterial metal nanoparticles, like silver nanoparticles, zinc oxide nanoparticles, and titanium oxide nanoparticles, have been incorporated into membranous ECM scaffolds to produce advanced wound dressings (Table 2) [100][101][102]114,115]. Among them, silver nanoparticles have been widely used in the treatment of acute and chronic wounds.…”

Section: Loading With Antibacterial Agentsmentioning

confidence: 99%

“…Zinc oxide nanoparticles, another frequently used agent to avoid wound infection, are compatible with biological system, and their biocompatibility and antimicrobial activity are related to the particle size [116]. After loading with zinc oxide nanoparticles, it was observed that AAM wound dressings showed a dose-dependent antibacterial activity of Gram-positive (S. mutans, S. aureus, L. fusiformis, and E. faecalis) and Gram-negative bacteria (P. vulgaris, S. sonnei, C. freundii, and P. aeruginosa) [102]. In addition to drugs and nanoparticles, antiseptic peptides have been utilized to generate antibacterial biomaterials for wound healing.…”

Section: Loading With Antibacterial Agentsmentioning

confidence: 99%

Membranous Extracellular Matrix-Based Scaffolds for Skin Wound Healing

Huang

Xie

et al. 2021

Pharmaceutics

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Membranous extracellular matrix (ECM)-based scaffolds are one of the most promising biomaterials for skin wound healing, some of which, such as acellular dermal matrix, small intestinal submucosa, and amniotic membrane, have been clinically applied to treat chronic wounds with acceptable outcomes. Nevertheless, the wide clinical applications are always hindered by the poor mechanical properties, the uncontrollable degradation, and other factors after implantation. To highlight the feasible strategies to overcome the limitations, in this review, we first outline the current clinical use of traditional membranous ECM scaffolds for skin wound healing and briefly introduce the possible repair mechanisms; then, we discuss their potential limitations and further summarize recent advances in the scaffold modification and fabrication technologies that have been applied to engineer new ECM-based membranes. With the development of scaffold modification approaches, nanotechnology and material manufacturing techniques, various types of advanced ECM-based membranes have been reported in the literature. Importantly, they possess much better properties for skin wound healing, and would become promising candidates for future clinical translation.

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Bio-Fabrication of Human Amniotic Membrane Zinc Oxide Nanoparticles and the Wet/Dry HAM Dressing Membrane for Wound Healing

Abstract: Graphical AbstractFlow chart depicting the methods, preparing, and characterizing, by histological, and scanning electron microscopy, of wet (PW-HAM) and dry (PD-HAM)of wound healing dressing, and preparation of nanoparticles (HAMP ZnO NP); and application of HAM wound dressing.

Cited by 13 publications

References 61 publications

Human Acellular Amniotic Membrane as Skin Substitute and Biological Scaffold: A Review of Its Preparation, Preclinical Research, and Clinical Application

Human Acellular Amniotic Membrane as Skin Substitute and Biological Scaffold: A Review of Its Preparation, Preclinical Research, and Clinical Application

Human amniotic membrane as a multifunctional biomaterial: recent advances and applications

Membranous Extracellular Matrix-Based Scaffolds for Skin Wound Healing

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