Bio-functionalization of metal–organic frameworks by covalent protein conjugation

Abstract

Bioconjugation of functional proteins onto metal-organic frameworks (MOFs) has been achieved using activation of pendent linking groups of the organic linkers on the surface of MOFs. Fluorescent microscopy revealed successful conjugation of an enhanced fluorescent protein onto MOFs. In addition, Candida-antarctica-lipase-B-conjugated MOFs showed no loss of enantioselectivity and activity in transesterification of (±)-1-phenylethanol.

Hierarchical Micro‐ and Mesoporous Zn‐Based Metal–Organic Frameworks Templated by Hydrogels: Their Use for Enzyme Immobilization and Catalysis of Knoevenagel Reaction

“…Immobilization of enzymes in MOFs can be achieved via surface attachment, covalent linkage, coprecipitation, and pore entrapment . The former two options in which enzymes were immobilized on the surface of MOFs via the formation of noncovalent interactions (van der Waals interaction, π–π stacking, or hydrogen bonds) or covalent bonds are the most straightforward . However, these methods do not benefit from the porous properties of MOFs since the pores are not utilized.…”

Application of Metal–Organic Frameworks in Fine Chemical Synthesis

“…More recently, Park and coworkers [132] reported the bioconjugation of functional proteins on the external surface of metal-organic frameworks through postsynthetic modification and studied the behavior of three MOFs having different dimensionalities ( Figure 10.23). The 1D MOF was prepared by reaction between In(NO 3 ) 3 and 1,4-phenylenediacetic acid, while the 2D MOF, [Zn(bpydc)(H 2 O)·(H 2 O)] n , is composed of zinc ions and 2,2 -bipyridine 5,5 -dicarboxylate (bpydc), and IRMOF-3 was used as the 3D MOF.…”

“…Canivet et al [149] successfully applied the solid-phase peptide synthesis to the postfunctionalization of the (In) MIL-68-NH 2 in order to anchor d-alanine and l-proline inside the MOF cavities (Figure 10.27). Following another strategy, Park [132] reported the grafting of functional proteins on the pendant carboxylic groups at the crystallite surface of different MOFs, while keeping the properties of the proteins intact. …”

A Microfluidic DNA Sensor Based on Three-Dimensional (3D) Hierarchical MoS2/Carbon Nanotube Nanocomposites

“…Over the last two decades, various nanomaterials including graphene oxide [1,2,3,4], Au nanoparticles [5,6,7], metal-organic framework (MOF) [8,9,10], carbon nanotubes [11,12], and MoS 2 nanosheets [13,14,15,16,17,18,19] have been synthesized to develop nanomaterial-based biosensors for a variety of applications. In particular, there has been increasing interest in developing rapid, cost-effective, and sensitive methods for DNA detection because of its vital importance in molecular diagnostics, environmental monitoring, and anti-bioterrorism [3].…”