Bio-guided fractionation of methanol extract of Ziziphus mauritiana Lam. (bark) and effect of the most active fraction on cancer cell lines

Tagne, Richard Simo; Telefo, Bruno Phelix; Talla, Emmanuel; Nyemb, Jean Noël; Njina, Sylvain Nguedia; Asrar, Mudassir; Mukhtar, Farah; Kamdje, Armel Hervé Nwabo; Moundipa, Paul Fewou; Farooq, Ahsana Dar; Choudhary, M. Iqbal

doi:10.1016/s2222-1808(14)60788-x

Cited by 8 publications

(5 citation statements)

References 19 publications

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“…Adebayo et al [37] found that the hexane fraction of the crude 80% ethanolic extract of Moringa oleifera had stronger antiproliferation activity against cancer cells compared to the crude extract. Similarly, hexane, dichloromethane, ethyl acetate, and butanol fractions of the crude methanolic extract of Ziziphus mauritiana bark had high inhibitory effects on MCF7, HeLa, prostate cancer (PC3), and lung cancer (NCI-H460) cells [38].…”

Section: Antiproliferative Assaymentioning

confidence: 99%

Antioxidant Effects, Antiproliferative Effects, and Molecular Docking of Clinacanthus nutans Leaf Extracts

et al. 2020

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Clinacanthus nutans is a well-known herb that has been used as an alternative and therapeutic medicine, however more selective C. nutans extracts are needed. In this study, leaves were extracted with 80% methanol and further fractionated with n-hexane, dichloromethane, chloroform, n-butanol, and aqueous residue. Subsequently, the total phenolic content (TPC), total flavonoid content (TFC), antioxidant scavenging activity, and antiproliferative effects on breast cancer (Michigan Cancer Foundation-7 [MCF7]) and normal breast (Michigan Cancer Foundation-10A [MCF 10A]) cells of the extracts were measured. Additionally, molecular docking simulation of the major compounds from C. nutans extracts was conducted. The aqueous residue had the highest TPC and TFC, whereas the crude extract had the highest scavenging activity. Among the extracts, dichloromethane extract (CN-Dcm) was selected as it had the highest selectivity index (SI) (1.48). Then, the chosen extract (CN-Dcm) was proceed for further analysis. The compounds from CN-Dcm were identified using gas chromatography–mass spectrometry (GC-MS). The major compounds from CN-Dcm were further investigated through molecular docking studies. Palmitic acid and linolenyl alcohol were the compounds found in the CN-Dcm extract that exhibited the highest binding affinities with p53-binding protein Mdm-2. These results highlight the potential of C. nutans as a source of anticancer activities.

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Section: Antiproliferative Assaymentioning

confidence: 99%

Antioxidant Effects, Antiproliferative Effects, and Molecular Docking of Clinacanthus nutans Leaf Extracts

et al. 2020

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“… Akanda and Hasan, 2021 Bark Methanol extract Antiproliferative activity Thehighest antiproliferative activity of methanol bark extract was observed against lung cancer (GI 50 = 28 ± 0.90 μg/mL) and breast cancer (GI 50 = 29.00 ± 0.90 μg/mL) cells, followed by prostate cancer (GI 50 = 39.87 ± 0.19 μg/mL) and uterus cancer (GI 50 = 37.10 ± 0.47 μg/mL)cells by inhibiting cell growth. Tagne et al, 2015 Leaf and bark Ethanol extract Antimicrobial activity Significantantimicrobial activity was observed for leaf and bark ethanolic extract against S. aureus, Pseudomonas aeruginosa, & Candida albicans. Mohamadou et al, 2021 Leaf Aqueous extract Antioxidant activity Pre-treatment of rats with 200, 400, or 100 mg/kg body weight of aqueous leaf extract of Ziziphus mauritiana decreased ALT, AST, ALP, and TB and increased catalase, glutathione peroxidase, reductase, and superoxide dismutase activity compared to alcohol administered group.…”

Section: Nutraceutical Propertiesmentioning

confidence: 99%

Indian jujube a potential fruit tree to improve the livelihood

Sishu

Das

Selvaraj

2023

Saudi Journal of Biological Sciences

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“…Considering the BA concentration in root bark extract (491.9 mg g −1 extract), the determined IC 50 value would be 2.1 µg of BA mL −1 , which is clearly lower than the toxicity described in the literature for BA (IC 50 within the 10-31 µg mL −1 concentration range) [39,41], suggesting the presence of a synergistic interaction between BA and other components of the extract, namely, oleanolic acid. In fact, BA, together with lupeol, was previously suggested to be responsible for the significant antiproliferative activity of a DCM bark extract from Ziziphus mauritiana against MCF-7 cells (IC 50 = 5 µg mL −1 ) [42]. A synthetic mixture of BA plus OA in the same proportion as that in the root bark extract (97.1%BA:2.9%OA) presented a lower antiproliferative effect (IC 50 of 15.27 µg mL −1 ) in the studied MDA-MB-231 cell line, underlining the synergetic contributions of other presented compounds in addition to OA.…”

Section: Biological Activities Of Lipophilic Z Lotus Extracts 231 Ant...mentioning

confidence: 99%

Chemical Profile of Lipophilic Fractions of Different Parts of Zizyphus lotus L. by GC-MS and Evaluation of Their Antiproliferative and Antibacterial Activities

et al. 2022

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Zizyphus lotus L. is a perennial shrub particularly used in Algerian folk medicine, but little is known concerning the lipophilic compounds in the most frequently used parts, namely, root bark, pulp, leaves and seeds, which are associated with health benefits. In this vein, the lipophilic fractions of these morphological parts of Z. lotus from Morocco were studied by gas chromatography–mass spectrometry (GC–MS), and their antiproliferative and antimicrobial activities were evaluated. GC–MS analysis allowed the identification and quantification of 99 lipophilic compounds, including fatty acids, long-chain aliphatic alcohols, pentacyclic triterpenic compounds, sterols, monoglycerides, aromatic compounds and other minor components. Lipophilic extracts of pulp, leaves and seeds were revealed to be mainly composed of fatty acids, representing 54.3–88.6% of the total compounds detected. The leaves and seeds were particularly rich in unsaturated fatty acids, namely, (9Z,12Z)-octadeca-9,12-dienoic acid (2431 mg kg−1 of dry weight) and (9Z)-octadec-9-enoic acid (6255 mg kg−1 of dry weight). In contrast, root bark contained a high content of pentacyclic triterpenic compounds, particularly betulinic acid, accounting for 9838 mg kg−1 of dry weight. Root bark extract showed promising antiproliferative activity against a triple-negative breast cancer cell line, MDA-MB-231, with a half-maximal inhibitory concentration (IC50) = 4.23 ± 0.18 µg mL−1 of extract. Leaf extract displayed interesting antimicrobial activity against Escherichia coli, methicillin-sensitive Staphylococcus aureus and Staphylococcus epidermis, presenting minimum inhibitory concentration (MIC) values from 1024 to 2048 µg mL−1 of extract. Our results demonstrate that Zizyphus lotus L. is a source of promising bioactive components, which can be exploited as natural ingredients in pharmaceutical formulations.

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Bio-guided fractionation of methanol extract of Ziziphus mauritiana Lam. (bark) and effect of the most active fraction on cancer cell lines

Cited by 8 publications

References 19 publications

Antioxidant Effects, Antiproliferative Effects, and Molecular Docking of Clinacanthus nutans Leaf Extracts

Antioxidant Effects, Antiproliferative Effects, and Molecular Docking of Clinacanthus nutans Leaf Extracts

Indian jujube a potential fruit tree to improve the livelihood

Chemical Profile of Lipophilic Fractions of Different Parts of Zizyphus lotus L. by GC-MS and Evaluation of Their Antiproliferative and Antibacterial Activities

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