Bio-inspired hydrogel composed of hyaluronic acid and alginate as a potential bioink for 3D bioprinting of articular cartilage engineering constructs

Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

“…Moreover, the generation of functional articular cartilage is challenging concerning the zonal structure of native tissue, including areas with different cell morphologies and arrangements, ECM arrangements, constituents, and distribution [177,178]. The introduction of 3D bioprinting in TE has attained prominent progress in simulating the anatomy of articular cartilage tissue [179], and among various dispensing techniques, EBB is the most prevalent and affordable method [180,181]. Applying this particular technique, researchers have reported the production of cartilage-like constructs through the combination of various hydrogels [46,[182][183][184][185][186][187][188]; However, the most efficient strategy has involved simultaneous deposition of thermoplastic polymers utilizing multi-dispenser systems while structural materials able of maintaining mechanical forces, and hydrogels as cell carriers [189][190][191][192][193][194].…”

Multicomponent polysaccharide alginate-based bioinks

“…Cartilage scaffolds. The applicability of HA-alginate based bioinks for the fabrication of articular cartilaginous constructs was explored by Antich et al 152 They prepared a bioink formulation containing 1.0% wt/vol HA and 2.0% wt/vol alginate, which was cross-linked with a 100 mM CaCl 2 solution. The polymer blend displayed good printability, gelling ability, optimal stiffness and degradability.…”

3D Bioprinting in Tissue Engineering for Medical Applications: The Classic and the Hybrid

“…The porous material mixture was shown to be suitable for ear tissue regeneration with the occurrence of chondrogenesis and adipogenesis [ 145 ]. A newly formulated HA and alginate-based bioink were reported by C. Antich et al for articular cartilage regeneration [ 146 ]. This bioink showed improved cell functionality (over 85% preserved cell viability after printing and increased GAG amount from around 23 μg/mL to 41.37 μg/mL after one month in culture) and promising 3D printability, mechanical properties, and biodegradability.…”