Bio-/Nanoimmobilization Platform Based on Bioinspired Fibrin-Bone@Polydopamine-Shell Adhesive Composites for Biosensing

Abstract

Inspired by blood coagulation and mussel adhesion, we report novel adhesive fibrin-bone@polydopamine (PDA)-shell composite matrix as highly efficient immobilization platform for biomacromolecules and nanomaterials. Fibrin, as a bioglue, and PDA, as a chemical adhesive, are integrated in a one-pot simultaneous polymerization consisting of biopolymerization of fibrinogen and chemical polymerization of dopamine. Fibrin fibers act as adhesive bones to construct scaffold, while PDA coat on the scaffold to form adhesive shell, generating 3D porous composite matrix with unique bone@shell structure. Two types of enzymes (glucose oxidase and acetylcholinesterase) and Au nanoparticles were adopted as respective model biomolecules and nanomaterials to investigate the immobilization capability of the matrix. The bionanocomposites showed high efficiency in capturing nanoparticles and enzymes, as well as significant mass-transfer and biocatalysis efficiencies. Therefore, the bionanocomposites exhibited significant potential in biosensing of glucose and paraoxon with limits of detection down to 5.2 μM and 4 ppt, respectively. The biological−chemical-combined polymerization strategy and composite platform with high immobilization capacity and mass-transfer efficiency open up a novel way for the preparation of high-performance bionanocomposites for various applications, in particular, biosensing.

A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

“…From the comparison between the developed methodology and previous biosensors for paraoxon detection (Table 1), the LOD value obtained using our EST2 enzyme is comparable to, or lower than, other LOD values measured using other esterase-based biosensors. Specifically, AChE-based methodologies presented a very low LOD due to the high specificity of AChE enzyme to paraoxon [41][42][43][44][45][46][47][48][49][50]; however, the use of EST2 allowed to achieve a low detection limit approaching the performance of AChE, and better sensitivity if compared to other enzymes, as is the case of the organophosphate hydrolase (OPH) used in two biosensing assays performed by Jain et al [51]. Moreover, from the comparison with the reviewed literature, it emerges that our biosensor presents the lowest incubation time (1 min) compared to the other methodologies, and lower production cost being easily over-expressed and purified by a single thermo-precipitation step, increasing the performance efficiency in terms of time saving and costs.…”

Nanobiohybrid Material‐Based Bioelectronic Devices

“…According to the point of view discussed earlier, bioinspired nanobiohybrid materials composed of fibrin, and polydopamine (PDA), enzymes, and GNPs were developed for highly efficient immobilizing and sensing platform for biomacromolecules. [ 57 ] In this research, to generate the 3D porous matrix with the unique bone@shell structure by using the proposed nanobiohybrid materials, the fibrin and PDA of nanobiohybrid materials acted as the adhesive bones to construct the scaffold, and the coating material for forming adhesive shell on the scaffold, respectively. Moreover, enzymes and GNPs were utilized to verify the immobilization capacity of the proposed matrix and used as the biosensing probe for the detection of target molecules.…”

Microenvironment responsive nanocomposite hydrogel with NIR photothermal therapy, vascularization and anti-inflammation for diabetic infected wound healing

“…Many inorganic materials (such as gold nanoparticle, graphene, carbon nanotube and transition metal dichalcogenide) and organic materials (small molecular agents such as Indocyanine Green (ICG), IR-825, and polymers such as polypyrrole, polyaniline, dopamine (PDA)) have been reported as PTT biomaterials [ [10] , [11] , [12] ]. As a melanin analogue naturally existing in the body [ 13 , 14 ], PDA has the most extensive application for PTT due to the intriguing properties such as simple synthetic method [ 15 , 16 ], good anti-oxidation property [ 17 ], adhesive performance [ 18 ] and biocompatibility. More importantly, due to the chelation interaction with metal ions, PDA is also a good carrier [ 19 ], which can slowly release metal ions into the wound and avoid the biotoxicity due to their excessive usage [ 20 , 21 ].…”