To establish a long-term sustainability of biodiesel to fulfill the quest for renewable fuel resources for the countries has low or no fossil fuels reserves. The energy resource meets to its limited capacity, alternative new energy resources must be explored such as the introduction of biodiesel as an alternative energy resources of petroleum products. The present research work is a 90-hour long-term endurance test performed with B30 sample (biodiesel 30% + 70% diesel) fueled with modified investigation performance, sound pressure level and engine durability. Special attention has been given to certain phenomena affecting the life of the engine, the formation of deposits and its performance. Experimental results of this research work demonstrated that the modified diesel engine could be efficiently performed with the B30 fuel sample.