Bioaccessibility and antioxidant activity of β-carotene loaded nanostructured lipid carrier (NLC) from binary mixtures of palm stearin and palm olein

Rohmah, Miftakhur; Rahmadi, Anton; Raharjo, Sri

doi:10.1016/j.heliyon.2022.e08913

Cited by 31 publications

(16 citation statements)

References 40 publications

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“…Among these, it is important to remember that: the DPPH-antioxidant reaction is reversible to some extent; therefore, the measured antioxidant capacity may be lower than it should be; DPPH reagent shows low accessibility due to high steric hindrance; therefore, large molecules may not be able to access the DPPH radical site [ 24 ]. Some authors reported that the ABTS assay is more responsive than DPPH for the evaluation of the antioxidant activity of carotenoid extracts because ABTS correlates positively with their free radical scavenging activity compared to the DPPH assay [ 25 ]. All these considerations additionally support our DPPH results.…”

Section: Resultsmentioning

confidence: 99%

“…SLN meet this need because they are composed of lipids, which are essential nutrients of the human diet and show good tolerability [ 38 ]. Lipid-based carriers are able to increase the bioaccessibility and the bioavailability of carotenoids [ 25 ]. In fact, nanoparticles can enhance carotenoid absorption by improving their solubility compared to the raw crystalline compound thanks to the greater surface-to-volume ratio, by prolonging the retention time, or through the direct absorption of the delivery systems.…”

Section: Resultsmentioning

confidence: 99%

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Unconventional Extraction of Total Non-Polar Carotenoids from Pumpkin Pulp and Their Nanoencapsulation

et al. 2022

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Pumpkin is considered a functional food with beneficial effects on human health due to the presence of interesting bioactives. In this research, the impact of unconventional ultrasound-assisted extraction (UAE) and microwave-assisted extraction techniques on the recovery of total non-polar carotenoids from Cucurbita moschata pulp was investigated. A binary (hexane:isopropanol, 60:40 v/v) and a ternary (hexane:acetone:ethanol, 50:25:25 v/v/v) mixture were tested. The extracts were characterized for their antioxidant properties by in vitro assays, while the carotenoid profiling was determined by high-performance liquid chromatography coupled with a diode array detector. UAE with the binary mixture (30 min, 45 °C) was the most successful extracting technique, taking into consideration all analytical data and their correlations. In parallel, solid lipid nanoparticles (SLN) were optimized for the encapsulation of the extract, using β-carotene as a reference compound. SLN, loaded with up to 1% β-carotene, had dimensions (~350 nm) compatible with increased intestinal absorption. Additionally, the ABTS ((2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay showed that the technological process did not change the antioxidant capacity of β-carotene. These SLN will be used to load an even higher percentage of the extract without affecting their dimensions due to its liquid nature and higher miscibility with the lipid with respect to the solid β-carotene.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Unconventional Extraction of Total Non-Polar Carotenoids from Pumpkin Pulp and Their Nanoencapsulation

et al. 2022

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“…In particular, 1 mg/ml of NE_NLCs correspond to 0.208 ± 0.003 ng/ml of Trolox equivalent, while 1 mg/ml of NLCs to 0.054 ± 0.002 ng/ml of Trolox equivalent antioxidant capacity. The mild antioxidant activity of the bare NLCs is most probably due to the presence of oleic acid and glyceryl dibehenate, as already highlighted in the literature (Rohmah et al, 2022).…”

Section: Preparation and Characterization Of Nlcs And Ne_nlcsmentioning

confidence: 51%

Hazelnut extract-loaded nanostructured lipid carriers and evaluation of their antioxidant properties

Emanet

Şen

Pignatelli

et al. 2022

Front. Bioeng. Biotechnol.

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Reactive oxygen species (ROS) are a common hallmark of many degenerative diseases, developing in all those cases where a failure of physiological antioxidant mechanisms occurs (in particular, antioxidant enzymes and the glutathione system), or in case of exposure to an extremely high level of oxidants. In this regard, antioxidant natural extracts are promising compounds as preventive or therapeutic agents against ROS-dependent degenerations. In this study, a deep investigation of hazelnut (Corylus avellana) extract has been performed in terms of mass spectroscopy, evaluation of phenolic content, and antioxidant capacity. Then, nanostructured lipid carriers (NLCs) have been exploited for encapsulation of the hazelnut extracts in order to achieve prolonged bioactivity, increased stability, and targeting through a sustainable delivery approach. The hazelnut extract-loaded NLCs (NE_NLCs) have been deeply characterized for their stability, production yield, and encapsulation efficiency. Moreover, NE_NLCs showed optimal cytocompatibility on human dermal fibroblast (HDF) cells, as well as excellent antioxidant activity, upon pro-oxidant stimulus on HDF cells.

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“…In a previous report on co‐encapsulated liposomes of vitamin C and βC, the free radical scavenging rates of L‐VC‐βC, including DPPH, ABTS and OH radicals, were significantly higher than that of L‐βC, but had no significant difference from that of L‐VC 9 . In some other reports of βC loaded nanocarriers, for example, βC loaded nanostructured lipid carrier (NLC) from palm stearin and palm olein, fibre‐encapsulated carotene nanocapsules, only slight increases of radical scavenging rates were reported compared with unloaded βC 48,49 . Taken together, co‐encapsulation of VE and βC could improve the radical scavenging activities significantly, especially the combination at 5:3 with the highest antioxidant activities.…”

Section: Resultsmentioning

confidence: 88%

“…9 In some other reports of ⊎C loaded nanocarriers, for example, ⊎C loaded nanostructured lipid carrier (NLC) from palm stearin and palm olein, fibre-encapsulated carotene nanocapsules, only slight increases of radical scavenging rates were reported compared with unloaded ⊎C. 48,49 Taken together, co-encapsulation of VE and ⊎C could improve the radical scavenging activities significantly, especially the combination at 5:3 with the highest antioxidant activities.…”

Section: Differential Scanning Calorimetrymentioning

confidence: 95%

Physicochemical properties, antioxidant activities and in vitro sustained release behaviour of co‐encapsulated liposomes as vehicle for vitamin E and β‐carotene

Zhang

Cheng

et al. 2022

J Sci Food Agric

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BACKGROUND In this study the potential of liposomes as a vitamin E (VE) and β‐carotene (βC) delivery system was examined. The co‐encapsulated liposomes of βC and VE (L‐VE‐βC) were prepared and characterized. Their antioxidant properties were evaluated by free radical scavenging activities of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS), hydroxyl radical and lipid peroxidation assay. The in vitro sustained release behaviour was then investigated and discussed. RESULTS VE and βC were co‐encapsulated in liposomes with high encapsulation efficiency, up to 92.49% and 86.16% for βC and VE, respectively. The antioxidant activities of L‐VE‐βC samples were significantly higher than that of single loaded liposome. Among different ratios of VE/βC, L‐VE‐βC at 5:3 exhibited the highest radical scavenging rates, with 66.80%, 56.58% and 34.39% for DPPH, ABTS and OH radical, respectively. L‐VE‐βC samples also had a good ability to inhibit lipid peroxidation, especially the sample with ratios of VE/βC at 5:3 and 3:1. In simulated gastrointestinal release, L‐VE‐βC exhibited an excellent sustained release behaviour in SGF with the accumulated rate at about 20%, while the release rate in SIF increased to over 80%, where they should be absorbed. The release kinetics analysis indicated that βC was released in the Higuchi model in stomach, and the Korsmeyr–Peppas model in intestine. CONCLUSION Compared to single loaded liposomes, the combined‐loaded liposomes exhibited higher antioxidant activity and bioavailability, suggesting the potential applications in functional foods. © 2022 Society of Chemical Industry.

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Bioaccessibility and antioxidant activity of β-carotene loaded nanostructured lipid carrier (NLC) from binary mixtures of palm stearin and palm olein

Cited by 31 publications

References 40 publications

Unconventional Extraction of Total Non-Polar Carotenoids from Pumpkin Pulp and Their Nanoencapsulation

Unconventional Extraction of Total Non-Polar Carotenoids from Pumpkin Pulp and Their Nanoencapsulation

Hazelnut extract-loaded nanostructured lipid carriers and evaluation of their antioxidant properties

Physicochemical properties, antioxidant activities and in vitro sustained release behaviour of co‐encapsulated liposomes as vehicle for vitamin E and β‐carotene

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