Bioaccumulation of the Selected Metal Ions in Saccharomyces cerevisiae Cells Under Treatment of the Culture with Pulsed Electric Field (PEF)

Pankiewicz, Urszula; Sujka, Monika; Jamroz, Jerzy

doi:10.1007/s00232-015-9844-3

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…To prevent these adverse changes, the microencapsulation process is used [10]. One of the encapsulation methods is the accumulation of minerals in the yeast cells of S. cerevisiae [11][12][13][14]. In the study of Kyyaly et al [15] it was demonstrated that feeding anemic rats with iron-enriched yeast is more efficient than inorganic treatment in recovery from iron deficiency.…”

Section: Of 19mentioning

confidence: 99%

Pulsed Electric Field (PEF) Enhances Iron Uptake by the Yeast Saccharomyces cerevisiae

et al. 2021

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The aim of the study was to investigate the influence of a pulsed electric field (PEF) on the level of iron ion accumulation in Saccharomyces cerevisiae cells and to select PEF conditions optimal for the highest uptake of this element. Iron ions were accumulated most efficiently when their source was iron (III) nitrate. When the following conditions of PEF treatment were used: voltage 1500 V, pulse width 10 μs, treatment time 20 min, and a number of pulses 1200, accumulation of iron ions in the cells from a 20 h-culture reached a maximum value of 48.01 mg/g dry mass. Application of the optimal PEF conditions thus increased iron accumulation in cells by 157% as compared to the sample enriched with iron without PEF. The second derivative of the FTIR spectra of iron-loaded and -unloaded yeast cells allowed us to determine the functional groups which may be involved in metal ion binding. The exposure of cells to PEF treatment only slightly influenced the biomass and cell viability. However, iron-enriched yeast (both with or without PEF) showed lower fermentative activity than a control sample. Thus obtained yeast biomass containing a high amount of incorporated iron may serve as an alternative to pharmacological supplementation in the state of iron deficiency.

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Section: Of 19mentioning

confidence: 99%

Pulsed Electric Field (PEF) Enhances Iron Uptake by the Yeast Saccharomyces cerevisiae

et al. 2021

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“…Prevention or treatment of iron deficiency can be achieved by enriching microorganisms (for example, yeast) used in the food industry with iron ions through the application of pulsed electric field (PEF) [ 8 ]. Previous studies have shown that using this technique increases the efficiency of ion accumulation by yeast from the medium [ 9 , 10 , 11 , 12 , 13 , 14 ]. Higher ion accumulation in cells results from the increased permeability of the cell membrane due to the phenomenon of electroporation.…”

Section: Introductionmentioning

confidence: 99%

The Use of Iron-Enriched Yeast for the Production of Flatbread

Nowosad

Sujka

2021

Molecules

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The most common cause of iron deficiency is an improperly balanced diet, in which the body’s need for iron cannot be met by absorption of this element from food. Targeted iron supplementation and food fortification may be the main treatments for iron deficiency in the population. However, many iron-rich supplements and foods have low bioavailability of this element. In our study, we used yeast enriched with iron ions to produce flatbread. The yeast cells accumulated iron ions from the medium supplemented with Fe(NO3)3·9H2O, additionally one of the cultures was treated with pulsed electric field in order to increase the accumulation. The potential bioavailability of iron from flatbread containing 385.8 ± 4.12 mg of iron in 100 g dry mass was 10.83 ± 0.94%. All the flatbreads had a moderate glycemic index. There were no significant differences in antioxidant activity against DPPH• between flatbread with iron-enriched and non-iron-enriched yeast. Sensory evaluation showed that this product is acceptable to consumers since no metallic aftertaste was detected. Iron enriched flatbread can potentially be an alternative to dietary supplements in iron deficiency states.

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“…Employing living microorganisms for metal biosorption has an advantage of simultaneously exploiting their inherent ability of absorbing and accumulating heavy metals intracellularly, a process known as bioaccumulation (Pankiewicz et al, 2015). Recently, a system of continuous growth of S. cerevisiae was demonstrated to be an efficient method of removing copper and lead ions from water (Amirnia et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Bioremediation potential of Cd by transgenic yeast expressing a metallothionein gene from Populus trichocarpa

Oliveira

Ullah

Dunwell

et al. 2020

Ecotoxicology and Environmental Safety

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Cadmium (Cd) is an extremely toxic environmental pollutant with high mobility in soils, which can contaminate groundwater, increasing its risk of entering the food chain. Yeast biosorption can be a low-cost and effective method for removing Cd from contaminated aqueous solutions.We transformed wild-type Saccharomyces cerevisiae (WT) with two versions of a Populus trichocarpa gene (PtMT2b) coding for a metallothionein: one with the original sequence (PtMT2b 'C') and the other with a mutated sequence, with an amino acid substitution (C3Y, named here: PtMT2b 'Y'). WT and both transformed yeasts were grown under Cd stress, in agar (0; 10; 20; 50 µM Cd) and liquid medium (0; 10; 20 µM Cd). Yeast growth was assessed visually and by spectrometry OD600. Cd removal from contaminated media and intracellular accumulation were also quantified. PtMT2b 'Y' was also inserted into mutant strains: fet3fet4, zrt1zrt2 and smf1, and grown under Fe-, Zn-and Mn-deficient media, respectively. Yeast strains had similar growth under 0 µM, but differed under 20 µM Cd, the order of tolerance was: WT < PtMT2b 'C' < PtMT2b 'Y', the latter presenting 37% higher growth than the strain with PtMT2b 'C'. It also extracted ~80% of the Cd in solution, and had higher intracellular Cd than WT. Mutant yeasts carrying PtMT2b 'Y' had slightly higher growth in Mn-and Fedeficient media than their non-transgenic counterparts, suggesting the transgenic protein may chelate these metals. S. cerevisiae carrying the altered poplar gene offers potential for bioremediation of Cd from wastewaters or other contaminated liquids.

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Bioaccumulation of the Selected Metal Ions in Saccharomyces cerevisiae Cells Under Treatment of the Culture with Pulsed Electric Field (PEF)

Cited by 7 publications

References 20 publications

Pulsed Electric Field (PEF) Enhances Iron Uptake by the Yeast Saccharomyces cerevisiae

Pulsed Electric Field (PEF) Enhances Iron Uptake by the Yeast Saccharomyces cerevisiae

The Use of Iron-Enriched Yeast for the Production of Flatbread

Bioremediation potential of Cd by transgenic yeast expressing a metallothionein gene from Populus trichocarpa

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