Bioaccumulation test with Tubificid Sludgeworms in artificial media development of a standardisable method

Egeler, Philipp; Römbke, Jörg; Meller, Michael; Knacker, Th.; Nagel, Roland

doi:10.1007/978-94-011-4207-6_27

Cited by 8 publications

(9 citation statements)

References 22 publications

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“…They were washed with distilled water prior to testing. For testing, the worms were kept in plastic cups half-filled with sediment and standard water (15 animals/50 ml, supplemented with 222 mgL −1 CaCl 2 , 123 mgL −1 MgSO 4 , 64 mgL −1 NaHCO 3 , 5.75 mgL −1 KCl) (Egeler et al 1999) for 14 days. Dichlorophenol (20 mgL −1 ) was used as a positive control for mortality.…”

Section: Bioassay With T Tubifexmentioning

confidence: 99%

Biochemical parameters in Tubifex tubifex as an integral part of complex sediment toxicity assessment

et al. 2008

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Background, aim, and scope Restoration of lakes and reservoirs with extensive cyanobacterial water bloom often requires evaluation of the sediment quality. Next to the chemical analysis of known pollutants, sediment bioassays should be employed to assess toxicity of the present contaminants and to make predictions of associated risk. Brno reservoir in the Czech Republic is a typical example of water bodies with long-term problems concerning cyanobacterial water blooms. Comprehensive assessment of reservoir sediment quality was conducted since successful reservoir restoration might require sediment removal. An important part of this survey focused on an examination of the utility of Tubifex tubifex and its sublethal biochemical markers for the assessment of direct sediment toxicity. Materials and methods This complex study included chemical analysis of contaminants (heavy metals, organic pollutants), ecotoxicity testing of sediment elutriates (tests with Daphnia magna, Pseudomonas putida, Sinapis alba, Scenedesmus subspicatus), and other parameters. We have tested in more detail the applicability of T. tubifex as a test organism for direct evaluation of contact sediment toxicity. Survival tests after 14 days of exposure were complemented by an assessment of parameters serving as biomarkers for sublethal effects [such as total glutathione content (GSH), activities of the enzymes glutathione transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR)]. The data matrix was subjected to multivariate analysis to interpret relationships between different parameters and possible differences among locations. Results The multivariate statistical techniques helped to clearly identify the more contaminated upstream sites and separate them from the less contaminated and reference samples. The data document closer relationships of the detected sediment contamination with results of direct sediment exposure in the T. tubifex test regarding mortality but namely regarding the sublethal endpoints rather than the results obtained with other test organisms exposed to sediment elutriates. Aside from the reduction in T. tubifex survival, the sediments with organic pollution caused an increase in glutathione content and increased activities of glutathione S-transferase and glutathione peroxidase in the exposed T. tubifex worms. Discussion Results of our study confirm the suitability of T. tubifex for toxicity testing of raw waters and sediments. This longer-lasting direct contact test has proven more sensitive and appropriate to reflect a lower level of pollution than do the elutriate tests. Sensitive biochemical changes in T. tubifex, including an elevation in GSH levels and GST activities, reflect a general stimulation of detoxification metabolisms in the presence of xenobiotics. The results also suggest an important role of glutathione and related enzymes in detoxification processes and possible involvement of oxidative stress in toxicity mechanisms in benthic sedimentdwelling worms such as T. tubifex. Conclusion...

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Section: Bioassay With T Tubifexmentioning

confidence: 99%

Biochemical parameters in Tubifex tubifex as an integral part of complex sediment toxicity assessment

et al. 2008

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“…This species can be identified easily by its posterior gills, and it is quite common and abundant in the field [30]. Branchiura sowerbyi can be cultured [31] and tested in the laboratory [32].…”

Section: Resultsmentioning

confidence: 99%

Using aquatic macroinvertebrate species traits to build test batteries for sediment toxicity assessment: Accounting for the diversity of potential biological responses to toxicants

Ducrot¹,

Usseglio‐Polatera²,

Péry³

et al. 2005

Enviro Toxic and Chemistry

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An original species-selection method for the building of test batteries is presented. This method is based on the statistical analysis of the biological and ecological trait patterns of species. It has been applied to build a macroinvertebrate test battery for the assessment of sediment toxicity, which efficiently describes the diversity of benthic macroinvertebrate biological responses to toxicants in a large European lowland river. First, 109 potential representatives of benthic communities of European lowland rivers were selected from a list of 479 taxa, considering 11 biological traits accounting for the main routes of exposure to a sediment-bound toxicant and eight ecological traits providing an adequate description of habitat characteristics used by the taxa. Second, their biological and ecological trait patterns were compared using coinertia analysis. This comparison allowed the clustering of taxa into groups of organisms that exhibited similar life-history characteristics, physiological and behavioral features, and similar habitat use. Groups exhibited various sizes (7-35 taxa), taxonomic compositions, and biological and ecological features. Main differences among group characteristics concerned morphology, substrate preferendum and habitat utilization, nutritional features, maximal size, and life-history strategy. Third, the best representatives of the mean biological and ecological characteristics of each group were included in the test battery. The final selection was composed of Chironomus riparius (Insecta: Diptera), Branchiura sowerbyi (Oligochaeta: Tubificidae), Lumbriculus variegatus (Oligochaeta: Lumbriculidae), Valvata piscinalis (Gastropoda: Valvatidae), and Sericostoma personatum (Trichoptera: Sericostomatidae). This approach permitted the biological and ecological variety of the battery to be maximized. Because biological and ecological traits of taxa determine species sensitivity, such maximization should permit the battery to better account for the sensitivity range within a community.

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“…He was the coordinator of two large EU projects, Terrestrial Model Ecosystems and ERAPharm (2004)(2005)(2006)(2007). Although Thomas was formally responsible for aquatic ecotoxicology at ECT GmbH, his research interests were much broader [4][5][6][7]. His influence was probably most visible in the environmental risk assessment of chemicals [8][9][10], such as their regulation in the marine environment (OSPAR process), the effects of endocrine disruptors [11,12] or their testing in higher-tier studies, especially Terrestrial Model Ecosystems (TMEs) [13][14][15].…”

Section: Dr Jörg Römbke Dr Anja Coorsmentioning

confidence: 99%

A farewell to Dr Thomas Knacker (* 29.04.1951 – † 30.10.2011): scientific contributions and personal memories

Römbke

Coors

Fenner³

et al. 2012

Environ Sci Eur

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Bioaccumulation test with Tubificid Sludgeworms in artificial media development of a standardisable method

Cited by 8 publications

References 22 publications

Biochemical parameters in Tubifex tubifex as an integral part of complex sediment toxicity assessment

Biochemical parameters in Tubifex tubifex as an integral part of complex sediment toxicity assessment

Using aquatic macroinvertebrate species traits to build test batteries for sediment toxicity assessment: Accounting for the diversity of potential biological responses to toxicants

A farewell to Dr Thomas Knacker (* 29.04.1951 – † 30.10.2011): scientific contributions and personal memories

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