Bioactive and Biodegradable Nanocomposites and Hybrid Biomaterials for Bone Regeneration

Allo, Bedilu; Costa, Daniel O.; Dixon, S. Jeffrey; Mequanint, Kibret; Rizkalla, Amin S.

doi:10.3390/jfb3020432

Cited by 131 publications

(77 citation statements)

References 162 publications

(240 reference statements)

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“…The rat skulls fixed in 10% formalin solution were decalcified using 5% HNO 3 . The tissues were embedded in a paraffin block and serially cut using a microtome (HM 325; Thermo Scientific, Rockford, IL).…”

Section: In Vivo Studymentioning

confidence: 99%

“…1,2 An ideal scaffold should be biocompatible and biodegradable, and function in a similar manner to the extracellular matrix (ECM). 3 Fibrous scaffolds fabricated by electrospinning have been used recently for biomedical applications because they can easily create a three-dimensional porous structure resembling the morphology of natural ECM. [4][5][6] The microstructure of electrospun scaffolds such as fiber diameter and porosity can be controlled by adjusting solution properties and operating parameters.…”

mentioning

confidence: 99%

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In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Kim

Linh

Min

et al. 2014

Tissue Engineering Part A

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To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds yielded an extracellular matrix-like microstructure that was a hybrid system composed of nano-and micro-sized fibers. BMP-2 was homogeneously loaded on the PCL-Gel-BCP scaffolds for enhanced induction of bone growth. BMP-2 was initially released at high levels, and then showed sustained release behavior for 31 days. Compared with the PCL-Gel-BCP scaffold, the BMP-2-loaded PCL-Gel-BCP scaffold showed improved cell proliferation and cell adhesion behavior. Both scaffold types were implanted in rat skull defects for 4 and 8 weeks to evaluate the biological response under physiological conditions. Remarkable bone regeneration was observed in the BMP-2/PCL-Gel-BCP group. These results suggest that BMP-2-loaded PCL-Gel-BCP scaffolds should be considered for potential bone tissue engineering applications.

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Section: In Vivo Studymentioning

confidence: 99%

mentioning

confidence: 99%

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Kim

Linh

Min

et al. 2014

Tissue Engineering Part A

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show abstract

“…Both inorganic and organic biomaterials (such as ceramics and polymers, respectively) have been widely used to prepare scaffolds with those requirements [3,[5][6][7][8][9]. Among ceramics, bioactive glasses-particularly Bioglass w 45S5-exhibit interesting properties for BTE [7,8,[10][11][12][13][14], such as the promotion of the formation of bone-like hydroxyapatite (HA) layers on the biomaterial surface [15,16], and their osteoconductive and osteoinductive characteristics [6,17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Biomaterials that resemble the calcified bone matrix are frequently used to develop scaffolds for bone tissue engineering (BTE) [1][2][3][4]. Both inorganic and organic biomaterials (such as ceramics and polymers, respectively) have been widely used to prepare scaffolds with those requirements [3,[5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

Cattalini

Hoppe

Pishbin

et al. 2015

J. R. Soc. Interface.

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This work aimed to develop novel composite biomaterials for bone tissue engineering (BTE) made of bioactive glass nanoparticles (Nbg) and alginate cross-linked with Cu 2þ or Ca 2þ (AlgNbgCu, AlgNbgCa, respectively). Twodimensional scaffolds were prepared and the nanocomposite biomaterials were characterized in terms of morphology, mechanical strength, bioactivity, biodegradability, swelling capacity, release profile of the cross-linking cations and angiogenic properties. It was found that both Cu 2þ and Ca 2þ are released in a controlled and sustained manner with no burst release observed. Finally, in vitro results indicated that the bioactive ions released from both nanocomposite biomaterials were able to stimulate the differentiation of rat bone marrow-derived mesenchymal stem cells towards the osteogenic lineage. In addition, the typical endothelial cell property of forming tubes in Matrigel was observed for human umbilical vein endothelial cells when in contact with the novel biomaterials, particularly AlgNbgCu, which indicates their angiogenic properties. Hence, novel nanocomposite biomaterials made of Nbg and alginate cross-linked with Cu 2þ or Ca 2þ were developed with potential applications for preparation of multifunctional scaffolds for BTE.

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“…[10] Artificial recombinant GFs require synthetic or animal proteins as carriers, but platelet-rich plasma (PRP) is a natural carrier itself. [11] Addition of PRP [12,13] or blood [14] enhances the osteoinductivity [12] and osteoconductivity [13] or leads to increased vascularization of implants, [14] whereas blood clots combined with macrophages have a beneficial effect on angiogenesis and organic bone matrix formation.…”

Section: Introductionmentioning

confidence: 99%

Effects of bone tissue engineering triad components on vascularization process: comparative gene expression and histological evaluation in an ectopic bone-forming model

Najdanović

Cvetković

Stojanović

et al. 2016

Biotechnology & Biotechnological Equipment

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Vascular development has a great significance in the osteogenic process and in bone tissue engineering (BTE). BTE is based on various combinations of three principal types of components: biomaterials as scaffolds, regulatory signals and cells. The aim of our study was to evaluate, at gene expression and histological level, the effect of BTE triad components on the vascularization process in an ectopic bone-forming model in mice. Bone mineral matrix (BMM) was used as a scaffold and a carrier, platelet-rich plasma (PRP) as a source of regulatory signals and adipose stem cells (ASCs) as a source of cells for endothelial differentiation, in order to show how and to what extent the biological enrichment of BMM influences the outcome of the osteogenic process and its key precondition, vascularization. Implants composed of BMM, PRP and ASCs in vitro induced into endothelial cells (EPB implants) and implants composed of BMM and PRP (PB implants) were compared with implants composed of BMM only (B implants). More pronounced endothelialrelated gene expression and stronger VCAM-1 (vascular cell adhesion molecule-1) immunoexpression were observed in EPB implants in comparison with PB and B ones at later time points of the in vivo experimental period. Osteopontin gene expression and immunoexpression of osteopontin were significantly higher in EPB compared to PB and B implants. Therefore, addition of ASCs combined with PRP to BMM improved the vascularization process in the ectopic boneforming model, which makes this BTE composition the most favourable among the examined types of implants for application in BTE.

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Bioactive and Biodegradable Nanocomposites and Hybrid Biomaterials for Bone Regeneration

Cited by 131 publications

References 162 publications

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

Effects of bone tissue engineering triad components on vascularization process: comparative gene expression and histological evaluation in an ectopic bone-forming model

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