Bioactive and Chemical Properties by Silkworm (Bombyx mori L.) Powder Degradation with Kiwifruit, Papaya, Pineapple and Pear Juice

Abstract

Bioactive and chemical properties of silkworm powder (SP) degradation by fruit extract containing the proteolytic enzymes of kiwifruit, papaya, pineapple and pear were investigated. Silkworm powder was incubated with extracts from each fruit at 60℃ for 24 hr. Protein content was slightly higher in the SP treated with fruit extract than that in the control SP. Major minerals were K, Ca, Mg, and Zn. Major fatty acids were linolenic acid, oleic acid, and palmitic acid. When total protein patterns were analyzed by SDS-polyacrylamide gel electrophoresis (PAGE), silkworm protein was strongly degraded by the treatment of fruit extract from pineapple, papaya, and pear, but little silkworm degradation was observed in kiwifruit extract treatment. Fibriolytic activity was only detected in the SP by the fruit extract treatments from papaya and pear. DPPH radical scavenging activity was slightly stronger in the SP treated with fruit extract than that in silkworm powder. However, all these samples exhibited a relatively low activity compared with the butylated hydroxytoluene (BHT). These results may provide the basic data for understanding the biological activities and chemical characteristics of SP treated with fruit extract for development of functional foods

Optimization of fermentation conditions Protaetia brevitarsis seulensis larvae using Bacillus subtilis

“…또한 고분자형태의 단백질을 저분자 단백질로 분해하여 다양한 생리활성 물질이 생성되 어 기존 소재에 비해 기능성이 향상된 식품재료로 인식 되고 있다 (9). 곤충 분말을 가지고 생리활성 작용이 우수한 B. subtilis로 발효 시킨 누에 분말 연구에서도 단백질 농도 가 증가하였으며, 항산화 작용뿐만 아니라 생리활성작용이 기존의 소재보다 증가한다는 결과를 밝힌 바 있고(9) 단백 질 분해력이 높은 균주를 이용하여 발효시킨 곤충 발효물에 서 항산화작용, 항혈전작용이 있는 것으로 보고됨에 따라 향후 건강식품 소재로 사용될 가치가 기대된다 (10 SN, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 0 day; SF1, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 1 day; SF2, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 2 days; SF3, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 3 days; SF4, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 4 days; SF5, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 5 days; SF6, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 6 days; SF7, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 7 days; SF8, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 8 days; SF9, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 9 days; SF10, P. brevitarsis seulensis larvae fermented using Bacillus subtilis for 10 days.…”