Bioactive bredigite coating with improved bonding strength, rapid apatite mineralization and excellent cytocompatibility

Abstract

Previous studies have shown that bredigite (Ca7MgSi4O16) bioceramics possessed excellent biocompatibility, apatite-mineralization ability and mechanical properties. In this paper, the bredigite coating on Ti-6Al-4 V substrate was prepared by plasma spraying technique. The main compositions of the coating were bredigite crystal phase with small parts of amorphous phases. The bonding strength of the coating to Ti-6Al-4 V substrate reached 49.8 MPa, which was significantly higher than that of hydroxyapatite coating and other silicate-based bioceramic coatings prepared by same method. After immersed in simulated body fluid for 2 days, a distinct apatite layer was deposited on the surface of bredigite coating, indicating that the prepared bredigite coating has excellent apatite-mineralization ability. The prepared bredigite coating supported the attachment and proliferation of rabbit bone marrow stem cells. The proliferation level of bone marrow stem cells was significantly higher than that on the hydroxyapatite coating. Our further study showed that the released SiO4 (4-) and Mg(2+) ions from bredigite coating as well as the formed nano-apatite layer on the coating surface might mainly contribute to the improvement of cell proliferation. The results indicated that the bredigite coating may be applied on orthopedic implants due to its excellent bonding strength, apatite mineralization and cytocompatibility.

In vitro degradability, bioactivity and primary cell responses to bone cements containing mesoporous magnesium–calcium silicate and calcium sulfate for bone regeneration

“…It was found that the apatite peaks (at approx. 2u ¼ 25.58, 32.18, 42.48 and 49.48) appeared on all the samples [5], and other peaks appeared at approximately 2u ¼ 20.88, 23.58, 29.28 and 47.98, which belong to the cements. Clearly, the intensity of the peaks for 40 m-CSBC (figure 6a) and 20 m-CSBC (figure 6b) were significantly higher than CSBC (figure 6c), which was in accord with the SEM images.…”

“…These characteristics made them one of the most intriguing materials in the biomedical field. The bredigite (Ca 7 MgSi 4 O 16 ) with apatite layer induced in simulated body fluid (SBF) could support cell attachment and stimulate cell proliferation and differentiation [5]. It was reported that some ions (such as calcium, magnesium and silicon) released from these bioceramics could change the biological environment in vivo and promote bone reconstruction [6].…”

The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cells

“…Bredigite, a silicate‐based bioceramic, has received significant attention because it can release bioactive ions and trigger specific cellular responses during tissue engineering . Bredigite possesses an advantage due to its relatively stable chemical and physical properties and controllable ion release compared with growth factors.…”

“…34 Bredigite, a silicate-based bioceramic, has received significant attention because it can release bioactive ions and trigger specific cellular responses during tissue engineering. 35 Bredigite possesses an advantage due to its relatively stable chemical and physical properties and controllable ion release 13 compared with growth factors. The release of bredigite ions after exposure to a physiological environment is well-defined and easy to control at the molecular level, 36 contributing to the validity of our results.…”

Degradability, cytocompatibility, and osteogenesis of porous scaffolds of nanobredigite and PCL–PEG–PCL composite

“…6,7 In addition, bredigite and its coating could promote the formation of bone-like apatite on material surface after soaking into stimulated body fluid. 8 Moreover, the osteoblasts could adhere and spread well on the surfaces of bredigite bioceramics, and the ionic product dissolution from bredigite obviously stimulated cell proliferation. 9,10 Therefore, bredigite-based biomaterials might open new possibilities for bone repair.…”