Bioactive compounds from Octopus vulgaris ink extracts exerted anti-proliferative and anti-inflammatory effects in vitro

Hernández-Zazueta, Martín Samuel; Luzardo-Ocampo, Iván; García-Romo, Joel Said; Noguera-Artiaga, Luis; Taboada-Antelo, Pablo; Campos-Vega, Rocío; Rosas‐Burgos, Ema Carina; Burboa-Zazueta, María Guadalupe; Ezquerra‐Brauer, Josafat Marina; Burgos‐Hernández, Armando

doi:10.1016/j.fct.2021.112119

Cited by 15 publications

(7 citation statements)

References 64 publications

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“…In addition, cephalopod ink secondary metabolites promoting immune function in vertebrates also showed different bioactive potentials, such as antibacterial, antimutagenic, and antitumoral activity [10]. Other studies evidenced that octopus ink extracts exhibited joint immunomodulatory and antiproliferative effects due to the presence of different bioactive compounds without being cytotoxic to human cancer cell lines [12]. Limited data are available regarding the activity of the bioactive peptides found in O. vulgaris ink; thus, this study may offer a new approach to identifying potential lead bioactive peptides from O. vulgaris ink.…”

Section: Discussionmentioning

confidence: 99%

“…Some of the previous studies reported that cephalopod ink extracts possess antimicrobial properties against diverse pathogenic bacteria [1,91]. Additionally, recently, it was found that O. vulgaris ink extracts exhibit anti-inflammatory, antiproliferative, antimutagenic, antioxidant, and cytoprotective properties [11,12]. There is currently no information concerning cephalopod ink bioactive peptides, but Nadarajah et al mentioned that fractions of melanin-free ink with low molecular weights (<3 kDa) showed the highest antioxidative activities [92].…”

Section: Discussionmentioning

confidence: 99%

“…The processing of squids, cuttlefish, and octopus generates large amounts of solid and liquid wastes in the forms of skin, head, cuttlebone, pen, ink, and viscera [10]. However, octopus ink by-products have received much attention due to the discovery of several relevant bioactive compounds [1, [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Insights into Common Octopus (Octopus vulgaris) Ink Proteome and Bioactive Peptides Using Proteomic Approaches

et al. 2023

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The common octopus (Octopus vulgaris) is nowadays the most demanded cephalopod species for human consumption. This species was also postulated for aquaculture diversification to supply its increasing demand in the market worldwide, which only relies on continuously declining field captures. In addition, they serve as model species for biomedical and behavioral studies. Body parts of marine species are usually removed before reaching the final consumer as by-products in order to improve preservation, reduce shipping weight, and increase product quality. These by-products have recently attracted increasing attention due to the discovery of several relevant bioactive compounds. Particularly, the common octopus ink has been described as having antimicrobial and antioxidant properties, among others. In this study, the advanced proteomics discipline was applied to generate a common octopus reference proteome to screen potential bioactive peptides from fishing discards and by-products such as ink. A shotgun proteomics approach by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using an Orbitrap Elite instrument was used to create a reference dataset from octopus ink. A total of 1432 different peptides belonging to 361 non-redundant annotated proteins were identified. The final proteome compilation was investigated by integrated in silico studies, including gene ontology (GO) term enrichment, pathways, and network studies. Different immune functioning proteins involved in the innate immune system, such as ferritin, catalase, proteasome, Cu/Zn superoxide dismutase, calreticulin, disulfide isomerase, heat shock protein, etc., were found in ink protein networks. Additionally, the potential of bioactive peptides from octopus ink was addressed. These bioactive peptides can exert beneficial health properties such as antimicrobial, antioxidant, antihypertensive, and antitumoral properties and are therefore considered lead compounds for developing pharmacological, functional foods or nutraceuticals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Insights into Common Octopus (Octopus vulgaris) Ink Proteome and Bioactive Peptides Using Proteomic Approaches

et al. 2023

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“…The supernatant was discarded and 100 µL of Anexin-V was taken, which was prepared under the supplier’s conditions along with 100 µL of resuspended cells in DMEM medium supplemented with 1% SFB. They were incubated for 20 min at room temperature and analyzed in a Merck Muse Cell Analyzer (Merck KGaA, Darmstadt, Germany) [ 92 ].…”

Section: Methodsmentioning

confidence: 99%

Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA

et al. 2022

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The estrogenic receptor beta (ERβ) protects against carcinogenesis by stimulating apoptosis. Bisphenol A (BPA) is related to promoting cancer, and naringenin has chemoprotective activities both can bind to ERβ. Naringenin in the colon is metabolized by the microbiota. Cancer involves genetic and epigenetic mechanisms, including miRNAs. The objective of the present study was to evaluate the co-exposure effect of colonic in vitro fermented extract of naringenin (FEN) and BPA, to elucidate molecular effects in HT-29 colon cancer cell line. For this, we quantified genes related to the p53 signaling pathway as well as ERβ, miR-200c, and miR-141. As an important result, naringenin (IC50 250 µM) and FEN (IC50 37%) promoted intrinsic pathways of apoptosis through phosphatase and tensin homolog (PTEN) (+2.70, +1.72-fold, respectively) and CASP9 (+3.99, +2.03-fold, respectively) expression. BPA decreased the expression of PTEN (−3.46-fold) gene regulated by miR-200. We suggest that once co-exposed, cells undergo a greater stress forcing them to mediate other extrinsic apoptosis mechanisms associated with death domain FASL. In turn, these findings are related to the increase of ERβ (5.3-fold with naringenin and 13.67-fold with FEN) gene expression, important in the inhibition of carcinogenic development.

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“…Moreover, it also induced cell cycle arrest and even apoptosis of the cells (Raja et al, 2020). Anticancer properties were also observed from Octopus vulgaris ink extract that constrained colorectal cancer cell proliferation by inducing apoptosis (Hernández-Zazueta et al, 2021).…”

Section: Anticancer Peptidesmentioning

confidence: 95%

Bioactive peptides-derived from marine by-products: development, health benefits and potential application in biomedicine

Pratama¹,

Putra²,

Pangestuti³

et al. 2022

Fish Aquat Sci

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Increased fisheries products have raised by-products that are discarded due to low economic value. In addition, marine by-products are still rich in protein and nutritional value that have biological activities and give benefits to human health. Meanwhile, there is raised pressure for sustainability practices in marine industries to reduce waste and minimize the detrimental effect on the environment. Thus, valorization by-products through bioactive peptide mining are crucial. This review focus on various ways to obtain bioactive peptides from marine by-products through protein hydrolysis, for instance chemical hydrolysis (acid and based), biochemical hydrolysis (autolysis and enzymatic hydrolysis), microbial fermentation, and subcritical water hydrolysis. Nevertheless, these processes have benefits and drawbacks which need to be considered. This review also addresses various biological activities that are favorable in pharmaceutical industries, including antioxidant, antihypertensive, anticancer, anti-obesity, and other beneficial bioactivities. In addition, some potential marine resources of Indonesia for the marine biopeptide from their by-product or undesired marine commodities would be addressed as well.

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Bioactive compounds from Octopus vulgaris ink extracts exerted anti-proliferative and anti-inflammatory effects in vitro

Cited by 15 publications

References 64 publications

Insights into Common Octopus (Octopus vulgaris) Ink Proteome and Bioactive Peptides Using Proteomic Approaches

Insights into Common Octopus (Octopus vulgaris) Ink Proteome and Bioactive Peptides Using Proteomic Approaches

Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA

Bioactive peptides-derived from marine by-products: development, health benefits and potential application in biomedicine

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