BIOACTIVE COMPOUNDS IN BENGKOANG (Pachyrhizus erosus) AS ANTIOXIDANT AND TYROSINASE INHIBITING AGENTS

Abstract

In Indonesia, the roots of bengkoang (Phacyrhizus erosus) have been used as the excipient for sun screening and skin whitening paste. Since the active compounds exhibiting skin whitening or sun screening effect have not previously been studied, the aim of this study was to identify compounds with antioxidant and tyrosinase inhibitor activities. Soxhlet extraction was used as the method of isolation with petroleum ether as the solvent and it was followed by fractionation using ethyl acetate to obtain three isoflavonoids (i.e. daidzein (2); daidzein-7-O-ß-glucopyranose (3); 5-hydroxy-daidzein-7-O-ß-glucopyranose (4)), and a new pterocarpan (i. e. 8,9-furanyl-pterocarpan-3-ol (1)) which antioxidant activities (SC 50% values) of 2.11; 11.86; 0.69 and 7.86 respectively. All compounds showed tyrosinase inhibiting activities with IC 50 values of 4.38; 5.35; 7.49 and 22.20 mM, respectively for compound 4, 2, 1 and 3. These compounds can be used as antioxidant and skin whitening materials.

SCREENING OF ANTIOXIDANTS, ANTI-AGING AND TYROSINASE INHIBITORY ACTIVITIES OF ETHANOLIC AND ETHYL ACETATE EXTRACTS OF FRUIT FLESH AND FRUIT PEEL LANGSAT (Lansium domesticum Corr) IN VITRO

“…Penentuan penghambatan tyrosinase dilakukan dengan menggunakan mushroom tirosinase sebagai enzim dan L-dopa sebagai substrat serta asam kojat sebagai kontrol positif, seperti yang pernah dilakukan oleh Lukitaningsih (2014). Tabel 2 menunjukkan inhibitor konsentrasi pada 50% (IC50) aktivitas enzim tirosinase oleh sampel.…”

Antioxidant and Antityrosinase Activities of Ethanolic Pachyrhizuserosus Peel and Tuber Extract

“…Bioactive compounds of P. erosus can be used to decrease melanin production and can supress tyrosinase activity. 20 P. erosus had active compounds as antioxidant and skin whitening activities such as daidzein, daidzin, genistin, (8,9)-furanyl-pterocarpan-3-ol, 4-(2-(furane-2-yl)ethyl)-2-methyl-2,5dihydro-furane-3-carbaldehyde and 2-butoxy-2,5-bis(hydroxymethyl)-tetrahydrofurane-3,4diol. 20 P. erosus compounds may have phenol or diphenol group which can form a chelate complex with copper (Cu 2+ ions).…”

“…20 P. erosus compounds may have phenol or diphenol group which can form a chelate complex with copper (Cu 2+ ions). 8 PTE may have a high amount of daidzein-7-O-ß-glucopyranose compared to PPE causing a lower inhibitory activity. 8 Daidzein-7-O-ß-glucopyranose had only one phenol group because the other phenol groups bond to a glucose molecule which formed a weak complex resulting in lower inhibitory activity.…”

“…8 PTE may have a high amount of daidzein-7-O-ß-glucopyranose compared to PPE causing a lower inhibitory activity. 8 Daidzein-7-O-ß-glucopyranose had only one phenol group because the other phenol groups bond to a glucose molecule which formed a weak complex resulting in lower inhibitory activity. 8 Besides, DPPH scavenging activity and tyrosinase inhibition of PPE are higher than PTE that presumably due to the combination of activities between compounds and secondary metabolites contained therein.…”

STUDY OF BENGKUANG MASK FORMULATION (Pachyrhizus erosus L) WITH ETHANOL EXTRACT OF SWEET POTATO LEAVES (Ipomoea batatas L) AS ANTIOXIDANT

“…3 Bengkoang has a high moisture content and anti-inflammatory properties which functions to cool the skin from the feeling of dryness that attacks the skin due to very hot sun, by using bengkoang for facial masks so that facial skin is protected from feeling dry, brighter and appear fresh. 4 Bengkoang fruit masks can show a change that is to reduce wrinkles on facial skin by 100%. 5 Yam tubers contain whitening agents (whitening agents) that can whiten and eliminate black marks and pigmentation on the skin.…”