Bioactive Fraction from Datura stramonium Linn. Promotes Human immune Cells Mediated Cytotoxicity towards Lung and Breast Cancer Cells

Abstract

Aim: The aim of the present study was to evaluate immune modulatory effect of fractions of D. stramonium L. leaves on human peripheral blood mononuclear cells (PBMC) followed by assessment of cytotoxic abilities of immunomodulated PBMC toward cancer cells. Material and methods: Bioassay (PBMC proliferation) guided fractionation of methanolic leaf extract of D. stramonium was performed to get active fraction and LC-MS was performed to identify the phytocompounds present in the bioactive fraction. The immunomodulatory potential of D. stramonium active fraction was assessed by i) MTT microcytotoxicity assay using A549 (lung carcinomas) and MCF-7 (breast cancer) cell lines and ii) analyzing the production of IL-2 and IFN-γ by human PBMC in the presence of active fraction. Results: Chromatographic fractionation guided by PBMC proliferation assay of D. stramonium extract resulted in bioactive fraction (fraction-10) exhibiting significant immunostimulatory activity [EC 50 =19.1±1.5(µg/ml)] on human blood lymphocytes. Fraction-10 pretreated PBMC displayed enhanced cytotoxicity towards A549 and MCF-7 (59%±2.1% and 62%±2.3% at 1:20 effector:target ratio respectively). Moreover, fraction-10 also enhanced the secretion of IL-2 (8 fold) and IFN-γ (10 fold) by human PBMC. The preliminary phytochemical analysis of fraction-10 from D. stramonium showed the presence of terpenoids and steroids. LC-MS analysis depicted presence of four major phytoconstituents in fraction-10 as daturaolone, daturadiol, stigmasterol and sitosterol with corresponding mass spectrum (m/z) of 440, 442, 412 and 414 respectively. Conclusion: The present report concluded that active fraction-10 of D. stramonium possesses potential immunostimulators that are capable of enhancing anticancer responses of human blood lymphocytes.

Pharmacological, Biopesticide and Post-Harvest Loss Management Application of Jimsonweed (Datura Stramonium)

“…These findings were similar to those reported by Gupta et al [56], who investigated the cytotoxic effects of methanolic extracts of D. stramonium leaves on A549 and MCF7 cells, finding considerable immunostimulation [57]. The methanolic leaf extract of D. innoxia revealed a possible cytotoxic impact on MCF-7 human breast cancer cell lines, with an IC50 of 93.73 g/ml [57].…”

Secondary Metabolites and Biological Profiles of Datura Genus

“…These results were like those exposed by Gupta et al [95] where the cytotoxic effects of methanolic extracts of D. stramonium leaves against A549 and MCF7 were evaluated, presenting a significant immunostimulation [94]. D. innoxia methanolic leaf extract showed a potential cytotoxic effect on MCF-7 cell lines of human breast cancer with an IC50 of 93.73 µg/ml [94].…”

Datura stramonium essential oil composition and it’s immunostimulatory potential against colon cancer cells

“…This is the first report describing the immunoenhancing potential of the D. oil which could be due to the bioactive molecules present in the essential oil. Previously, it had been shown that D. stramonium leaves extract exhibited immunostimulatory potential on human lymphocytes (Gupta et al 2016).…”