Bioactive glass and hybrid scaffolds prepared by sol–gel method for bone tissue engineering

Pereira, Marivalda M.; Jones, Julian R.; Hench, Larry L.

doi:10.1179/174367605225011034

Cited by 124 publications

(87 citation statements)

References 52 publications

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“…They can be generally divided in two main groups: solid-state reactions and wet processes. The solid-state reactions include precipitation, hydrothermal technique and hydrolysis of other calcium phosphates (Mobasherpour et al 2007), while the wet route concerns mainly the sol-gel process (Pereira et al 2005;Xu et al 2006). Depending upon the route and technique used, HA bioceramics with various morphology, Ca/P ratios, microstructure and level of crystallinity can be obtained.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

et al. 2008

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The number of biomaterials used in biomedical applications has rapidly increased in the past two decades. Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetically prepared composite that in its structure contains the same molecular concentration of OH − groups and F − ions. The aim of this experimental investigation was to use the embryonal mouse fibroblast cell line NIH-3T3 for comparative study of basal cytotoxicity of fluoridated biomaterials FHA and FA discs. Hydroxyapatite (HA) disc, high-density polyethylene as negative control and polyvinyl chloride (PVC) containing organotin stabilizer as positive control were used as standard biomaterials. The appropriateness of the use of NIH-3T3 cells and their sensitivity for tested biomaterials were evaluated on the basis of five cytotoxic end points: cell proliferation, cell morphology, lactate dehydrogenase (LDH) released, protein and DNA cell content. The basal cytotoxicity of FHA, FA and HA discs was measured by direct contact method. FHA composite, FA and HA demonstrated in cell line NIH-3T3 nearly similar basal cytotoxicity increasing with the time of treatment. After 72 h of biomaterials treatment, about 25% inhibition of cell number, unchanged morphology of dividing cells, 6.31-0.16% increase of released LDH, about 10% inhibition of cell protein content and about 20% inhibition of DNA content was found. On the other hand, from the growth rates it resulted that NIH-3T3 cells, affected by tested biomaterials, divided about 20% slowlier than the control (untreated cells). Using the linear regression analysis we found out that deviations in measurements of cytotoxicity by four methods were as follows: less than 10% for cell number, protein and DNA content methods and 12.4% for released LDH method. Based on a good correlation of the cytotoxicity of biomaterials obtained from all end points we could conclude that fibroblast NIH-3T3 cell line was appropriate for measuring the basal cytoxicity of tested biomaterials.

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Section: Introductionmentioning

confidence: 99%

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

et al. 2008

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“…The successful design of ceramic bone biomaterials is challenged by two competing requirements (Pereira et al 2005;Cancedda et al 2007): on the one hand, such materials need to be stiff and strong, which would suggest a low porosity (of pore *Author for correspondence (christian.hellmich@tuwien.ac.at).…”

Section: Introductionmentioning

confidence: 99%

The role of disc-type crystal shape for micromechanical predictions of elasticity and strength of hydroxyapatite biomaterials

Fritsch

Hellmich

Dormieux

2010

Phil. Trans. R. Soc. A.

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The successful design of ceramic bone biomaterials is challenged by two competing requirements: on the one hand, such materials need to be stiff and strong, which would suggest a low porosity (of pore sizes in the 10-100 mm range) to be targeted; on the other hand, bone biomaterials need to be bioactive (in particular vascularized), which suggests a high porosity of such materials. Conclusively, reliable information on how porosity drives the stiffness and strength properties of ceramic bone biomaterials (tissue engineering scaffolds) is of great interest. In this context, mathematical models are increasingly being introduced into the field. Recently, self-consistent continuum micromechanics formulations have turned out as expressedly efficient and reliable tools to predict hydroxyapatite biomaterials' stiffness and strength, as a function of the biomaterialspecific porosity, and of the 'universal' properties of the individual hydroxyapatite crystals: their stiffness, strength and shape. However, the precise crystal shape can be suitably approximated by specific ellipsoidal shapes: while it was shown earlier that spherical shapes do not lead to satisfactory results, and that acicular shapes are an appropriate choice, we here concentrate on disc-type crystal shape as, besides needles, plates are often reported in micrographs of hydroxyapatite biomaterials. Discbased model predictions of a substantial set of experimental data on stiffness and strength of hydroxyapatite biomaterials almost attain the quality of the very satisfactory needle-based models. This suggests that, as long as the crystal shape is clearly nonspherical, its precise shape is of secondary importance if stiffness and strength of hydroxyapatite biomaterials are predicted on the basis of continuum micromechanics, from their micromorphology and porosity.

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“…They can be generally divided in two main groups: solid-state reactions and wet processes. The solid-state reactions include precipitation, hydrothermal technique and hydrolysis of other calcium phosphates [14], while the wet route mainly involves the sol-gel process [15,16]. Depending upon the route and technique used, HA bioceramics with various morphology, Ca/P ratios, microstructure and level of crystallinity can be obtained.…”

Section: Introductionmentioning

confidence: 99%

“…Synthetic HA-bioceramics are obtained by different chemical and technical processes [13][14][15][16]. They can be generally divided in two main groups: solid-state reactions and wet processes.…”

Section: Introductionmentioning

confidence: 99%

Potential applications of fluorhydroxyapatite as biomaterials in medicine

et al. 2009

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Bioactive glass and hybrid scaffolds prepared by sol–gel method for bone tissue engineering

Cited by 124 publications

References 52 publications

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

The role of disc-type crystal shape for micromechanical predictions of elasticity and strength of hydroxyapatite biomaterials

Potential applications of fluorhydroxyapatite as biomaterials in medicine

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