Bioactive glass promoted formation of nodules in periodontal‐ligament fibroblasts <i>in vitro</i>

Kubo, Kohji; Kakimoto, Takashi; Kanda, Chieko; Tsukasa, Nobuyuki; Uehara, Mayumi; Izumi, Yuichi; Kamada, Takenobu; Kaneko, Norio; Sueda, Takeshi

doi:10.1002/jbm.820270908

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…Phase B is the interfacial region for It is well established that scaffold chemistry and morphology modulate cell differentiation and matrix elaboration. 53,[62][63][64][65][66][67][68][69][70] Therefore, the triphasic nature of the scaffold is essential for the development of multiple tissue types on a single construct. The scaffold designed for this study include regions with varying morphology as well as chemistry with the specific intention of supporting multiple cell types.…”

Section: Discussionmentioning

confidence: 99%

Development of Controlled Matrix Heterogeneity on a Triphasic Scaffold for Orthopedic Interface Tissue Engineering

et al. 2006

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Biological fixation of orthopedic soft tissue grafts to bone poses a significant clinical challenge. The clinical success of soft tissue-based grafts for anterior cruciate ligament (ACL) reconstruction is limited by the lack of functional graft integration with subchondral bone. Soft tissues such as the ACL connect to subchondral bone via a complex interface whereby three distinct tissue regions (ligament, fibrocartilage, and bone) work in concert to facilitate load transfer from soft to hard tissue while minimizing stress concentration at the interface. Although a fibrovascular tissue forms at the graft-to-bone interface following surgery, this tissue is nonphysiologic and represents a weak link between the graft and bone. We propose that the re-establishment of the native multi-tissue interface is essential for biological graft fixation. In vivo observations and our in vitro monolayer co-culture results suggest that osteoblast-fibroblast interaction is important for interface regeneration. This study focuses on the design of a triphasic scaffold system mimicking the multi-tissue organization of the native ACL-to-bone interface and the evaluation of osteoblast-fibroblast interactions during three-dimensional co-culture on the triphasic scaffold. We found that the triphasic scaffold supported cell proliferation, migration and phenotypic matrix production while maintaining distinct cellular regions and phase-specific extracellular matrix deposition over time. This triphasic scaffold is designed to guide the eventual reestablishment of an anatomically oriented and mechanically functional fibrocartilage interfacial region directly on biological and synthetic soft tissue grafts. The results of this study demonstrate the feasibility of multi-tissue regeneration on a single scaffold, and the potential of interface tissue engineering to enable the biological fixation of soft tissue grafts to bone.

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Section: Discussionmentioning

confidence: 99%

Development of Controlled Matrix Heterogeneity on a Triphasic Scaffold for Orthopedic Interface Tissue Engineering

et al. 2006

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“…This result is in agreement with other studies which have used BG in cell culture. 20,21 Morphological analysis by fluorescence microscopy showed that cells grown on control surfaces, 2D and 2D+BG presented the same pattern of spreading and immunolabeling to ALP and OPN (observed only at 14 days), which suggests that the BG particles and their ionic dissolution products have no influence in these processes. Analysis by confocal microscopy showed cells adhered to the particles surfaces with altered morphology, and also a discrete intracellular ALP and OPN immunolabeling were observed at 7 and 14 days only in cells on 3D.…”

Section: Discussionmentioning

confidence: 95%

“…25,26 Althought no significant diference has been not observed, the BG showed a positive influence in ALP activity of hPDLF cells, which is in accordance with others researches that showed that ALP activity was greater in the fibroblasts cultured with BG than in the cells grown without them. 20,21 Calcium content was also favored by the presence of BG. It was higher on 2D+BG and 3D+BG culture, however no close relationship between the mineralized nodules and the particles was observed by microscopy.…”

Section: Discussionmentioning

confidence: 96%

“…Estudos mostraram que embora a proliferação celular tenha diminuído na presença do vidro bioativo e vitamina D3, a atividade de fosfatase alcalina e formação de nódulos mineralizados em culturas de fibroblastos do ligamento periodontal aumentaram (Kubo et al, 1993). Além disso, altas concentrações de cálcio e de silício liberadas a partir do vidro bioativo foram detectados no meio de cultura (Kubo et al, 1995), o que diz respeito à formação de nódulos sobre as células do ligamento periodontal .…”

Section: Vidro Bioativounclassified

“…Osteogenic cells were isolated by sequential trypsin/collagenase digestion of calvarial bone from newborn (2-4 days) Wistar rats, as previously described (17)(18)(19)(20).…”

Section: Cell Isolation and Primary Culture Of Osteogenic Cellsmentioning

confidence: 99%

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Expressão dos fenótipos fibroblástico e osteoblástico em culturas tridimensionais na presença de partículas de vidro bioativo

Alves¹

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Characterization of nodules induced by bioactive glass on cultured periodontal‐ligament fibroblasts

Kubo¹,

Kamada²,

Matsuyama³

et al. 1995

J. Biomed. Mater. Res.

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We previously reported that materials leached from bioactive glass (BG) and vitamin D3 induced the formation of nodules on cultured periodontal-ligament fibroblasts (PLF). In this study, we have investigated the relationship between the conditions of the materials and nodule formation, analyzed morphologically, and also studied whether the production of nodules was specific to cultured PLF. PLF and skin fibroblasts were cultured in the presence or absence of BG. The amounts of calcium, phosphate, sodium and silicon in the culture medium and the number of nodules were measured at the 55th day. The nodules were observed microscopically and analyzed using an X-ray microanalyzer. In PLF, nodules were formed regardless of the presence or absence of BG; however, they were more numerous in the presence of BG. In skin fibroblasts, nodules were not observed. The amounts of calcium and silicon were higher in the presence of BG, while the amount of phosphate was lower. The nodules appeared crystalline with a spongy structure and contained calcium and phosphorus. Our results show that the nodules were associated with PLF and precipitated by the materials (higher concentrations of calcium and silicon), and they were spongy crystal composed of calcium and phosphorus.

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Bioactive glass promoted formation of nodules in periodontal‐ligament fibroblasts in vitro

Cited by 10 publications

References 14 publications

Development of Controlled Matrix Heterogeneity on a Triphasic Scaffold for Orthopedic Interface Tissue Engineering

Development of Controlled Matrix Heterogeneity on a Triphasic Scaffold for Orthopedic Interface Tissue Engineering

Expressão dos fenótipos fibroblástico e osteoblástico em culturas tridimensionais na presença de partículas de vidro bioativo

Characterization of nodules induced by bioactive glass on cultured periodontal‐ligament fibroblasts

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