Bioactive nanoparticles stimulate bone tissue formation in bioprinted three‐dimensional scaffold and human mesenchymal stem cells

Gao, Guojun; Schilling, Arndt F.; Yonezawa, Tomo; Wang, Jiang; Dai, Guohao; Cui, Xiaofeng

doi:10.1002/biot.201400305

Cited by 302 publications

(195 citation statements)

References 41 publications

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“…Cells were expanded as previously reported [12,15]. Fifth passage (P5) hMSCs were used in this study.…”

Section: Human Mesenchymal Stem Cell Monolayer Expansionmentioning

confidence: 99%

“…RNA was isolated and purified Accepted Article Biotechnology Journal using RNeasy Plus Mini Kit (QIAGEN, Valencia, CA) following the manufacture protocol. As reported previously, quantitative gene expression was performed using the TaqMan RNA-to-C T 1-Step Kit [15]. For osteogenic differentiation, the gene expression of key osteogenic transcriptional factor RUNX2 (Hs00231692_m1), Osterix (SP7; Hs01866874_s1), Dlx5 …”

Section: Rna Isolation and Gene Expressionmentioning

confidence: 99%

“…For osteogenic differentiation, the minced samples were digested in pepsin and the total collagen and alkaline phosphatase activity were measured as previously reported [15]. For chondrogenic samples, total glycosaminoglycan (GAG) content was extracted using papain and measured as reported previously [9][10][11]40].…”

Section: Biochemical Assaymentioning

confidence: 99%

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Inkjet‐bioprinted acrylated peptides and PEG hydrogel with human mesenchymal stem cells promote robust bone and cartilage formation with minimal printhead clogging

Gao

Yonezawa

Hubbell³

et al. 2015

Biotechnology Journal

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297

184

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Inkjet bioprinting is one of the most promising additive manufacturing approaches for tissue fabrication with the advantages of high speed, high resolution, and low cost. The limitation of this technology is the potential damage to the printed cells and frequent clogging of the printhead. Here we developed acrylated peptides and co-printed with acrylated poly(ethylene glycol) (PEG) hydrogel with simultaneous photopolymerization. At the same time, the bone marrow-derived human mesenchymal stem cells (hMSCs) were precisely printed during the scaffold fabrication process so the cells were delivered simultaneously with minimal UV exposure. The multiple steps of scaffold synthesis and cell encapsulation were successfully combined into one single step using bioprinting. The resulted peptide-conjugated PEG scaffold demonstrated excellent biocompatibility, with a cell viability of 87.9 ± 5.3%. Nozzle clogging was minimized due to the low viscosity of the PEG polymer. With osteogenic and chondrogenic differentiation, the bioprinted bone and cartilage tissue demonstrated excellent mineral and cartilage matrix deposition, as well as significantly increased mechanical properties. Strikingly, the bioprinted PEG-peptide scaffold dramatically inhibited hMSC hypertrophy during chondrogenic differentiation. Collectively, bioprinted PEG-peptide scaffold and hMSCs significantly enhanced osteogenic and chondrogenic differentiation for robust bone and cartilage formation with minimal printhead clogging.

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“…Cells were expanded as previously reported [12,15]. Fifth passage (P5) hMSCs were used in this study.…”

Section: Human Mesenchymal Stem Cell Monolayer Expansionmentioning

confidence: 99%

Section: Rna Isolation and Gene Expressionmentioning

confidence: 99%

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Inkjet‐bioprinted acrylated peptides and PEG hydrogel with human mesenchymal stem cells promote robust bone and cartilage formation with minimal printhead clogging

Gao

Yonezawa

Hubbell³

et al. 2015

Biotechnology Journal

Self Cite

297

184

View full text Add to dashboard Cite

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“…Utilizing the specificity of BMP-2 micropatterns allowed the control of the differentiation of MDSCs. Similarly, the Cui and co-workers [110] engineered bone-like tissue with increased compressive modulus using a jet-based bioprinter. HMSCs, photopolymerizable PEGDMA, and HA nanoparticles were bioprinted in the same bioink at predefined 3D locations.…”

Section: Bioprinted Bonementioning

confidence: 99%

3D Miniaturization of Human Organs for Drug Discovery

Park

Wetzel

Dréau

et al. 2017

Adv Healthcare Materials

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“Engineered human organs” hold promises for predicting the effectiveness and accuracy of drug responses while reducing cost, time, and failure rates in clinical trials. Multiorgan human models utilize many aspects of currently available technologies including self‐organized spherical 3D human organoids, microfabricated 3D human organ chips, and 3D bioprinted human organ constructs to mimic key structural and functional properties of human organs. They enable precise control of multicellular activities, extracellular matrix (ECM) compositions, spatial distributions of cells, architectural organizations of ECM, and environmental cues. Thus, engineered human organs can provide the microstructures and biological functions of target organs and advantageously substitute multiscaled drug‐testing platforms including the current in vitro molecular assays, cell platforms, and in vivo models. This review provides an overview of advanced innovative designs based on the three main technologies used for organ construction leading to single and multiorgan systems useable for drug development. Current technological challenges and future perspectives are also discussed.

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“…Considering the advantages of polymer composite hydrogels, such as interpenetrating polymer networks (IPNs) or hybrid hydrogels [45], the incorporation of synthetic fillers to printable materials recently discussed in researches [33]. The addition of silicate fillers [38] and a range of nanoparticles have been used to synthesize different types of composite scaffolds [46] to promote greater control over viscosity and stiffness of polymer hydrogels.…”

Section: Composite Materialsmentioning

confidence: 99%

Application of 3-D Printing for Tissue Regeneration in Oral and Maxillofacial Surgery: What is Upcoming?

Keyhan¹,

Fallahi²,

Jahangirnia³

et al. 2018

Biomaterials in Regenerative Medicine

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The ultimate goal of any surgical procedure is to improve perioperative form and function and to minimize operative and postoperative morbidity. In recent years, many exciting and novel technological advances have been introduced in the field of oral and maxillofacial surgery. One example of such technology that is continuing to increase in prevalence is the use of 3-dimensional (3-D) printing techniques with special properties, which seems hopeful for practitioners in the field of regenerative medicine. Tissue engineering is a critical and important area in biomedical engineering for creating biological alternatives for grafts, implants, and prostheses. One of the main triad bases for tissue engineering is scaffolds, which play a great role for determining growth directions of stem cells in a 3-dimensional aspect. Mechanical strength of these scaffolds is critical as well as interconnected channels and controlled porosity or pores distribution. However, existing 3-D scaffolds proved less than ideal for actual clinical applications. In this chapter, we review the application and advancement of rapid prototyping (RP) techniques in the design and creation of synthetic scaffolds for use in tissue engineering. Also, we survey through new and novel merging era of "bioprinting."

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Bioactive nanoparticles stimulate bone tissue formation in bioprinted three‐dimensional scaffold and human mesenchymal stem cells

Cited by 302 publications

References 41 publications

Inkjet‐bioprinted acrylated peptides and PEG hydrogel with human mesenchymal stem cells promote robust bone and cartilage formation with minimal printhead clogging

Inkjet‐bioprinted acrylated peptides and PEG hydrogel with human mesenchymal stem cells promote robust bone and cartilage formation with minimal printhead clogging

3D Miniaturization of Human Organs for Drug Discovery

Application of 3-D Printing for Tissue Regeneration in Oral and Maxillofacial Surgery: What is Upcoming?

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