Bioactive phenolic derivatives from Acaena splendens methanol extract

Abstract

Acaena splendens H. et A. has been used in Chilean folk medicine for the treatment of fever and inflammation. A description of the in vivo reduction of bacterial pyrogen-induced fever in rabbits and carrageenan-induced paw oedema in guinea pigs is presented. The methanol extract named ME-1, obtained after succesive extractions with petroleum ether and dichloromethane, showed a strong antipyretic action (45.7% of effect), though the antiinflammatory activity was only observed after submitting this extract to column fractionation, giving a crude mixture of flavonoids named C4 with both activities (55.7% and 98.9% of antiinflammatory and antipyretic effect respectively at a dose of 600 mg/kg). The bioassay-guided fractionation by column chromatography afforded the active fraction, which contained (-,-)-epicatechin, tiliroside, 7-O-acetyl-3-O-beta-D-glucosyl-kaempferol and 7-beta-D-glucosyloxy-5-hydroxy-chromone.

Inhibitory Effects of the Flavonoids Isolated from Waltheria indica on the Production of NO, TNF-.ALPHA. and IL-12 in Activated Macrophages

“…The structures of (Ϫ)-epicatechin, quercetin, and tiliroside ( Fig. 1), were elucidated by detailed 1 H-and 13 C-NMR spectroscopy and by comparison of the spectral data with those published values, [8][9][10] and their molecular masses were 290, 302, and 594 g/mol, respectively.…”

Characterisation of polyphenols by HPLC-PAD-ESI/MS and antioxidant activity inEquisetum telmateia

“…Additionally, the well-documented antioxidant activity of this flavonol could also contribute to its antiatherogenic activity. Recently, Backhouse et al (2002) ascribed significant anti-pyretic and anti-inflammatory activities to an extract containing an acetylglucosyl derivative of kaempferol and (-)-epicatechin. Therefore, it is possible that the traditional anti-inflammatory uses of E. telmateia are due, at least in part, to the high content of such kaempferol derivatives.…”

Effect of Neuroprotective Flavonoids ofAgrimonia eupatoriaon Glutamate-Induced Oxidative Injury to HT22 Hippocampal Cells

“…Among these fractions, the EtOAc and n-BuOH fractions which showed the significant neuroprotective activity were used for isolating the active compounds. The EtOAc (22 g) fraction was subjected to CC over silica gel (300 g, 6 Â 60 cm), eluting with n-hexane- 15) allowed us to propose its structure as an acetyl derivative of the latter. The location of the acetate group at C-2 00 of the glucose was deduced from the HMBC spectrum.…”

“…The nine known compounds were identified as kaempferol 3-O--D-(2 00 -O-acetyl)glucopyranoside (2), tiliroside (3), astragalin (4), apigenin 7-O--D-glucuronide (5), rutin (6), isoquercitrin (7), quercitrin (8), luteolin 7-O--D-glucuronide (9), and luteolin 7-O--D-glucopyranoside (10) by comparing the physicochemical and spectroscopic data with previously reported data. [15][16][17][18][19] Among the ten isolated flavonoids, compound 1 was newly characterized as an acetyl derivative of tiliroside.…”