Characterisation of polyphenols by HPLC-PAD-ESI/MS and antioxidant activity inEquisetum telmateia

Abstract

The antioxidant activity of an aqueous extract (infusion) and respective ethyl acetate fraction of Equisetum telmateia Ehrh. (Equisetaceae), a plant used in traditional medicine for its anti-inflammatory and diuretic properties, has been evaluated by DPPH, TEAC and TBARS assays. A high and significant antioxidant activity was detected in the ethyl acetate fraction. Analysis of the aqueous extract and the ethyl acetate fraction by HPLC-PAD-ESI/MS allowed the identification of the major phenolic compounds as flavan-3-ol, kaempferol and phenolic acid derivatives. Among the flavan-3-ols, A-type proanthocyanidins and afzelechin derivatives were detected as well as the more common B-type procyanidins, B2 and C1, whose identification was further confirmed by HPLC using detection involving chemical reaction with p-dimethylamino-cinnamaldehyde. The results suggest that the anti-inflammatory activity of E. telmateia could be due, at least in part, to the presence of compounds with antioxidant activity.

Contribution of the phenolic composition to the antioxidant, anti-inflammatory and antitumor potential of Equisetum giganteum L. and Tilia platyphyllos Scop.

“…The detection of two peaks could be explained by the existence of trans-and cis-isomers of the p-coumaric acid, as also noted by Karioti et al 21 The most abundant peak 32 Tp can be assigned as trans-tiliroside taking into account that the form trans is the usual one for p-coumaric acid. unique fragment ion at m/z 285, from the loss of an acetylhexosyl residue, was assigned as kaempferol-3-O-acetylglucoside, a compound also reported by Correia et al 34 in E. telmateia.…”

Antioxidant, Antimicrobial and Genotoxicity Screening of Hydro-alcoholic Extracts of Five Serbian Equisetum Species

“…The obtained values for the total phenol content in the dry plant material (92-349 μmol per gram of dried plant material) are considerably higher than those previously published for Equiseti herba (60 μmol of catechin equivalents per g [7]), while the total phenol content of E. telmateia (infusion) was comparable to our results (ca. 460 μmol of gallic acid equivalents per g of dried plant material [8]). Expressed as the number of μmol of chlorogenic acid per g of the dry extract, the total phenol content ranges from 1,061 μmol/g for E. palustre to 1,531 μmol/g for E. sylvaticum.…”

“…Previously, only Equiseti herba was evaluated in the micronucleus test [6]. Antioxidant activity of E. arvense and E. telmateia extracts [2,7,8] and the antimicrobial activity of the essential oil of E. arvense [9] have been reported. HPLC profiles of methanolic extracts of E. arvense, E. sylvaticum, E. fluviatile, E. palustre and E. telmateia [10,11] were previously investigated, likewise the profile of the ethyl acetate fraction of E. telmateia infusion has been published recently [8].…”

Medicinal plants used for cardiovascular diseases in Navarra and their validation from Official sources

“…In reference to ethnopharmacological studies of this plant, it is interesting to point out that the distribution of its use is the same as Potentilla reptans: Andalusia, Aragon, Basque Country, Cantabria, Castile-La Mancha, Catalonia, Navarra, and Region of Murcia in Spain, and Italian Republic and Republic of Turkey (Akerreta, 2009, and references cited therein; Akerreta et al, 2013). The number of scientific references about the composition of Equisetum telmateia are few; it contains essential oils (Fons et al, 2013), various flavonoids and their glycosides, phenolic acid derivates and flavan-3-ol compounds (Correia et al, 2005) with possible antioxidant and medicinal properties.…”