Bioactive steroidal alkaloids from Buxus macowanii Oliv.

Lam, Cheuk W.; Wakeman, A. Maurice; James, Abin; Ata, Athar; Gengan, Robert Moonsamy; Ross, Samir A.

doi:10.1016/j.steroids.2014.12.002

Cited by 26 publications

(8 citation statements)

References 34 publications

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“…Most plants from the Buxus genus such as Buxus rugulose, are known to contain triterpenoid alkaloids which have different biological activities, including antimicrobial activity [36]. Additionally, five new steroidal alkaloids: 31-hydroxybuxatrienone, macowanioxazine, 16a-hydroxymacowanitriene, macowanitriene and macowamine as well as five known steroidal bases Nb-demethylpapillotrienine, moenjodaramine, irehine, buxbodine B and buxmicrophylline C have recently been isolated from B. macowanii [39]. Steroidal alkaloids are also known to cause cell membrane disruption and consequently, leakage of cytoplasmic contents [40].…”

Section: Discussionmentioning

confidence: 99%

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Disruption of microbial cell morphology by Buxus macowanii

Ngobeni

Mashele

Malebo

et al. 2020

BMC Complement Med Ther

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Background: Microbial infections are one of the major causes of death globally. This is attributed to the rising costs of primary healthcare and its inaccessibility especially in developing countries. Moreover, there has been an increase in microbial strains that have reduced susceptibility to antimicrobial drugs. Research on the antimicrobial properties of medicinal plants, which could address these problems, has become more important as they present fewer side effects when compared to the antibiotics currently in use. This study evaluated the antimicrobial properties of a methanolic extract from Buxus macowanii in order to assess its potential in the development of novel antimicrobial drugs. Methods: Antimicrobial activity of the extract was evaluated using the broth microdilution method. The effects of B. macowanii on the morphology of B. cereus were observed using Scanning and Transmission electron microscopy. Chemical profiling of the plant extract was performed using the GCMS. Results: The extract showed antimicrobial activity against all the microbial species used. Microscopic examination of the cells of B. cereus cells treated with Buxus macowanii showed some changes in morphology such as damage of the cell wall, swelling of the cells and incomplete cell division that eventually resulted in cell death. Neophytadiene, an antimicrobial compound was detected in the extract using GCMS. Conclusion: The morphological disruptions of the cell wall of Bacillus cereus explain the antimicrobial properties of B. macowanii and indicate its possible application in the development of natural antimicrobial drugs.

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Section: Discussionmentioning

confidence: 99%

“…1 b and c) observed on bacterial cells treated with B. macowanii. Furthermore, the steroidal alkaloids found in the plant have anti-AChE activity or showed prevention of excessive degradation of acetylcholine which is associated with Alzheimer’s disease [ 39 ], indicating a multipurpose remedy.…”

Section: Discussionmentioning

confidence: 99%

Disruption of microbial cell morphology by Buxus macowanii

Ngobeni

Mashele

Malebo

et al. 2020

BMC Complement Med Ther

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“…Lam et al isolated bioactive steroidal alkaloids from Buxus macowanii and tested for inhibitory activities against β-secretase. 27 Among them, 31-hydroxybuxatrienone and N b -demethylpapillotrienine showed inhibitory activities against β-secretase with IC 50 values of 15.0 and 25.0 µM, respectively ( Figure 26). Nguyen et al isolated 4 novel compounds and 9 known compounds from Lycopodiella cernua and tested for anti-β-secretase activities.…”

Section: Reports On β-Secretase Inhibitors Of Natural Originmentioning

confidence: 99%

Chemical Diversity of β-Secretase Inhibitors From Natural Resources

Murata

2019

Natural Product Communications

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Reports on β-secretase inhibitors of natural origin are listed in order to reveal their chemical diversity. Various types of compounds were found to inhibit β-secretase, and natural resources included a wide spectrum of biological species. Among them, some triterpenes and moracin derivatives, which are nonpeptidic compounds, were determined to be competitive inhibitors. In addition, no peptide compounds were reported from natural resources. These points will be clarified in future studies.

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“…It would be worthwhile to investigate medicinal plants used to treat wounds in folk medicines for the identification of new lead antimicrobial compounds. In our continuing efforts to discover new bioactive natural products from ethnomedicinal plants , we have collected stems and bark of D . staudtii from Nigeria based on its reported ethnomedicinal properties.…”

Section: Introductionmentioning

confidence: 99%

“…This plant has not been previously phytochemically investigated. Our antimicrobial-directed phytochemical studies on the crude MeOH extract of D. staudtii resulted in the isolation of two new compounds, 4,5-(methylenedioxy)-o-coumaroylputrescine (1) and 4,5-(methylenedioxy)-o-coumaroyl-4 0 -N-methylputrescine (2), along with seven known natural products 4a-hydroxyeremophila-1,9diene-3,8-dione (3), drypemolundein B (4), friedelan-3b-ol (5), erythrodiol (6), ursolic acid (7), p-coumaric acid (8), and b-sitosterol (9). Structures of compounds 1 -9 were determined with the aid of NMR and mass spectroscopic studies.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Compounds from Drypetes staudtii

Grace

Khan

Friesen

et al. 2016

Chemistry & Biodiversity

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Antimicrobial-directed phytochemical investigation of the MeOH extract of Drypetes staudtii afforded two new compounds, 4,5-(methylenedioxy)-o-coumaroylputrescine (1), 4,5-(methylenedioxy)-o-coumaroyl-4'-N-methylputrescine (2), along with seven known natural products 4α-hydroxyeremophila-1,9-diene-3,8-dione (3), drypemolundein B (4), friedelan-3β-ol (5), erythrodiol (6), ursolic acid (7), p-coumaric acid (8), and β-sitosterol (9). Structures of compounds 1 - 9 were elucidated with the aid of extensive NMR and mass spectral studies. All of the isolates exhibited antibacterial activity against Gram-positive and Gram-negative bacteria with minimum inhibitory concentration (MIC) in the range of 8 - 128 μg/ml. Compounds 1 - 2 were also moderately active against Candida albicans with an MIC value of 32 μg/ml.

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Bioactive steroidal alkaloids from Buxus macowanii Oliv.

Cited by 26 publications

References 34 publications

Disruption of microbial cell morphology by Buxus macowanii

Disruption of microbial cell morphology by Buxus macowanii

Chemical Diversity of β-Secretase Inhibitors From Natural Resources

Antimicrobial Compounds from Drypetes staudtii

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