Bioactivities of iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifolium

Fadel, Sezan R.; Bendıf, Hamdi; Guedes, Laura; André, Rebeca; Pacheco, Rita; Guedes, Rita; Merabti, Karim Elhadj; Mıara, Mohamed Djamel; Serralheiro, Maria Luísa

doi:10.1016/j.eujim.2020.101171

Cited by 7 publications

(7 citation statements)

References 43 publications

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“…Table 1 also has a tentative drawing of the structures to give an insight into its molecular design. These structures will help to explain any enzyme inhibitory activity and the antioxidant activity that may be detected with this extract when the enzyme inhibitory activity whose active site structure is known [1] and will help to propose through docking studies which will be the most appropriate inhibitor for the enzyme under evaluation.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The plant was collected in Algeria and prepared according to the process described in [1] . After filtration of the decoction, the aqueous extract was lyophilized, and a powder was obtained.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…A chromatographic column Intensity Solo 2 RP-18, 100 × 2.1 mm, 2.0 µm column (Bruker, Bremen, Germany) was used. The elution conditions were described in [1] , briefly: 5 µL were injected (auto injector) and a flow rate of 0.250 mL/min for the elution was used. The column was kept at 35°C and the samples at 10°C.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Data on identification of primary and secondary metabolites in aqueous extract of Verbascum betonicifolium

et al. 2020

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Tentative identification of primary and secondary metabolites in aqueous extracts from aerial parts of Verbascum betonicifolium Kuntze was done. This plant belongs to the Scrophulariaceae family and is used for several treatments in folk medicine. One of the processes commonly used to prepare this plant for consumption is boiling with water during approximately 20 minutes, that is, a decoction process. After filtration, this decoction was analysed in search for bioactive metabolites. The analysis was carried out by Electro-Spray Ionization (ESI) and High-Resolution Mass Spectrometry (HRMS) was done using a Quadropole Time-of-Flight (QToF, Impact II, Bruker), coupled to an Ultra High-Performance Liquid Chromatography (UHPLC, ELUTE autosampler, Bruker). The analysis was done in the negative mode (ESI-) and the identification was accomplished using the molecular formula suggestions from the Data Analysis 4.4™ software from Bruker and some databases, like Metlin and PubChem, always confirming with MS/MS results. These data can be used for finding biomarkers between Verbascum sps or to complementary medicine practitioners to get a scientific based knowledge of their results. These data are the unpublished supplementary materials related to “Bioactivities of Iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifolium ” (Fadel et al., 2020, submitted).

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Data on identification of primary and secondary metabolites in aqueous extract of Verbascum betonicifolium

et al. 2020

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“…The plants used for the development of new active ingredients must be the subject of rigorous scientific investigative work which requires multidisciplinary teamwork (chemists, biologists, pharmacists, veterinarian, etc.) (Fadel et al, 2020). Indeed, next to the classic primary metabolites (carbohydrates, proteins, lipids, and nucleic acids), they also synthesize a large number of other metabolites indirectly essential for plant life, called "secondary metabolites".…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening and Evaluation of the Antibacterial Activity of Galactites Tomentosa Extract

Boudjema¹,

Azeddine²,

Ikram³

et al. 2022

PLANT ARCHIVES

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The aim of current study was to investigate a wild plant, known by purple milk thistle (Galactites tomentosa), very abundant in El-Kala National Park and the Mediterranean basin. This study was designed to evaluate the antibacterial activity effects of the Galactites tomentosa. The extract was further analyzed by spectrophotometry, to measure the sugars (5,51 ± 0,04 mg/g DM), the proteins (14,86 ± 0,30 g/100g DM), polyphenols (12,24 ± 0,10 GAE mg/g DM), and chlorophyll pigments (1,13 ± 0,20 mg/g FM). This work is considered as an introduction to the valuation of El-Kala National Park (EKNP) plants, for the development of the food and pharmaceutical industry.

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“…Hypercholesterolemia, characterized by the presence of high levels of cholesterol in the blood caused by high intake of cholesterol, saturated fats, and excessive calories or by genetic factors [22], can lead to other diseases such as obesity, diabetes and cancer [23; 24].Cholesterol turn-over and homeostasis in the body involve many pathways including the inhibition of the rate-limiting enzyme 3hydroxy-3 La -methylglutaryl-coenzyme A (HMG-CoA) reductase [25] Currently, HMGR inhibitors are commonly used as e cient cholesterol-lowering drugs. In addition to statins, several phenolic compounds could t within the active site of the enzyme indicating that this may be one of the procedures by which cholesterol biosynthesis is inhibited [26; 27,28,29,30].…”

Section: Introductionmentioning

confidence: 99%

Diospyros Kaki Leaves Decoction Phytochemical Characterization and Bioactivities Evaluation: LC-MS-Qtof Identification, Antioxidant Activity, Enzyme Inhibition and Cytotoxicity Toward HepG2 and Mcf-7 Cell Lines

Ressaissi

Mannai

Ben‐Attia³

et al. 2021

Preprint

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Diospyros kaki known as Persimmon has played an important role in Chinese medicine, and its leaves are used in pharmaceuticals, cosmetics, and beverages due to their biological properties. The aim of this study was to evaluate chemical composition, antioxidant activity, enzyme inhibition. The main compounds of Diospyros kaki leaves decoctions were identified using high-resolution liquid chromatography – high-resolution tandem mass spectrometry (LC-HRMS-MS) interfaced with a Quadrupole Time-of-Flight (Q-tof). The obtained results showed that the aqueous extract had a high content of total phenolsand flavonoids and showed strong antioxidant activity (EC50 between 3.79±0.19 and 12.05±0.11µg/mL). In addition, these extracts were able to inhibit3-hydroxy-3-methylglutaril-CoA (HMG-CoA) reductase and acetylcholinesterase (IC50 ranging from 58.35±0.69 to 210.21±28.9 µg/mL). The cytotoxicity against human liver cells HepG2 and breast MCF7 cell lines was also evaluated. No cytotoxicity to HepG2 and MCF7 cells was detected. These results indicate that Diospyros kaki leaves could be considered as attractive source of health-promoting ingredients and a promising plant for the development of new functional foods and pharmaceutical products.

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Bioactivities of iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifolium

Cited by 7 publications

References 43 publications

Data on identification of primary and secondary metabolites in aqueous extract of Verbascum betonicifolium

Data on identification of primary and secondary metabolites in aqueous extract of Verbascum betonicifolium

Phytochemical Screening and Evaluation of the Antibacterial Activity of Galactites Tomentosa Extract

Diospyros Kaki Leaves Decoction Phytochemical Characterization and Bioactivities Evaluation: LC-MS-Qtof Identification, Antioxidant Activity, Enzyme Inhibition and Cytotoxicity Toward HepG2 and Mcf-7 Cell Lines

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